Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCAG_mRsph6a-FLAG

Expression vector of mouse Rsph6a.

Sequence (full)	RDB17453zzk02.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Abbasi, F et al. J. Cell Sci. 131(19) jcs221648, 2018)
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Abbasi, F et al. J. Cell Sci. 131(19) jcs221648, 2018)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

References and tips

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17453_A9G9p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 (Pr0164) Region: insert 5' Sequence file: RDB17453_A9G9a.seq
>D06337A5_A9Gg_3_pAxCA_F1_A03_03_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNCC TTCTTCTTTT TCNNNCNGCT CCTGGGCAAC GTGCTGGTTA    61 TTGTGCTGTC TCNTCATTTT GGCAAATCTA GAGCCGCCAT GGGGGAACCA CCGCCCAATC   121 CTGACCCCTC TCAAACCCGG AGGGCTTCCC AAGGCTCAGA AAGGGCACGG AGTCAAGAGT   181 ACTCTCAGCC TCTGTTAACC ATCCCAGAGG ATGGGTTGAA CCGACCACCT CCGCAGCGGG   241 GCAGTCGGAG CAGTCAGGGC AGTCAGGACC TGCAGGGAAC TGGTCTACCC CACTGGCCAC   301 AGAGGTCCAG CCTGGTCCCA GATGTTCAAG GTGATGAAGG CACCGAGTAC CACCAGTCCA   361 TGCCCTTAGG CTACACGCCA GGCTTTCCCA TGGAGTTCTC GCAACAGGGT TACCTGGATG   421 ATAGCAGGAT GATGGAGCAG TTCCCACAGG GCCAGGACCT CTTAGAACAA CTGGAGTCTA   481 CCTACCAAGG CTCGGCTTCT GGAATCCTGG GCCAGCTCAA CTTGTATCCA AGAGAGGATG   541 AGATATTCAG CCAGGACACA CAGCACGGAC CCTACCTGAG AGACGACCCC TCCCTCCACC   601 TGAGGCCCTC TGACCTGGGC TTCATGCCCA TTGTTGGAGA GGTACCTGAC CCCGAGCCTC   661 GTGAGCTGGC AATACAGAAC GCCAAGGCCT ACCTGCTGCG GANCAGCATG AGCTGCAACC   721 TCAGCCTGTA TGAGCACCTG GTGAACCTGC TGANCAAGAT CCTGAACCAG CGCCCTGANG   781 ANCCCTTGTC CATCCTGGAG TCTCTGAACC GCACCATGCA GTGGGAATGG TTTCACNNCA   841 AGCTGGACAC GCTGCGGGNN GANCNNNAGA TGCAGCCNAC NTACNANATG GNCGAAAAGC   901 AAAANNNTCT GTTTATCANG GGTGGNNGAN AAGGNGAANN GGNAATGGNA GANGANGNGN 961 NNGNNNNNNN NGNNNCNNNN NTNNNNGNNN NNNNNNN //
Primer: pCA-bgter (Pr0066) Region: FLAG, insert 3' Sequence file: RDB17453_A9G9b.seq
>D06337A5_A9Gg_3_pCA-bgter_B03_06_ABI24.ab1     1 NNNNNNNNNN NNNNNNTTTT GNNNGANNNN NNNGNNCTCN GTGGTNTTTG TGAGCCAGGG    61 CATTGGCCAC ACCAGCCACC ACCTTCTGAT AGGCAGCCTG CACCTGAGGA GTCTCGAGCT   121 ACTTGTCATC GTCATCCTTG TAGTCGATAT CGAATTCGTC CTCCAGGTCT TCATCCTCCT   181 CCTCTTCCTC ATCTTCCTCC TCTTCCTCAG CGGCTGCCAG GGCCTGCTCC TGAGCAGCCT   241 TCAGGGCCTG CTCCTCCTCC ACTGTGGGGT CACTCATCTC CGTGATTTCG GGGCCGCTGG   301 GGTACTCTTG CTGAATCAGA GCTGGCAGCA TTGGGTTGAA GTTCTCGGGG CTATACTTGT   361 GACCCCAGCC AATGTATATG TTCTCAAACT TCTTGCCAGT GGCGTAGGCA TAGGCCCCTG   421 GCCAGAGGTT GGAGCGCACG ATGGCCACCG AGTACTGAGG GCTGAGGCTG CAGGAGAGGC   481 GGGTGGTCCA GGGCGACAGG TGCATGATTT CTGCATCTTC TGAGAGCGGA GTCAGAAGCG   541 GAGGACCGAC CTCCTGCTCC ACCTCCTCCA TTGCCTCGTC TGCCTTCTCT TCCTCCTCCC   601 CTAGCTCTTC TTCCTCCTCT GTTTTCTGCA GTGGGTTCAC CCATGTACAG CGCCCCTGAG   661 GCAGGATGTG CTGAGTGTGA TGTACCCAGT TGGCCATGGA GTCCACGAGC TCNAACACGG   721 GGATGCCCTC AAAGTCGGGG TTCTCCTCAA AGGAATCGCG TCCCGCGCCG CCCTCCTCCT   781 CCTCATCGCC CTCCTCCTCG CCGAANTGGT ANAAGCCCAG CGGGCTGATG TGTGTGGCGG   841 CGGANANGCG CGCAATCTGC GCGCGGANAN AGTTGGNCTC GTNGCCCGGG AANGGCGGAT   901 ANCTGATGAN NGGCGNNNNC AGGANNNNAG NGAANAACTT TTTNGANNTT GCNCGCGCNC 961 NCNNTCTGCG CGGNNNNNNN NNN //
Primer: pUC-CAPbs_up_F (Pr0536) Region: lac pro, lacO, T3 pro, CMV en, CAG pro Sequence file: RDB17453_A9G9c.seq
>D06337A5_A9Gg_3_pUC-CAPbs_up_F_C03_09_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NCNNACGCAA TTAATGTGAG TTAGCTCNCT CATTAGGCAC    61 CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC   121 AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA GCGCGCAATT AACCCTCACT   181 AAAGGGAACA AAAGCTGGAG CTCATCGATA CTCGACATTG ATTATTGACT AGTTATTAAT   241 AGTAATCAAT TACGGGGTCA TTAGTTCATA GCCCATATAT GGAGTTCCGC GTTACATAAC   301 TTACGGTAAA TGGCCCGCCT GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAA   361 TGACGTATGT TCCCATAGTA ACGCCAATAG GGACTTTCCA TTGACGTCAA TGGGTGGAGT   421 ATTTACGGTA AACTGCCCAC TTGGCAGTAC ATCAAGTGTA TCATATGCCA AGTACGCCCC   481 CTATTGACGT CAATGACGGT AAATGGCCCG CCTGGCATTA TGCCCAGTAC ATGACCTTAT   541 GGGACTTTCC TACTTGGCAG TACATCTACG TATTAGTCAT CGCTATTACC ATGGTCGAGG   601 TGAGCCCCAC GTTCTGCTTC ACTCTCCCCA TCTCCCCCCC CTCCCCACCC CCAATTTTGT   661 ATTTATTTAT TTTTTAATTA TTTTGTGCAG CGATGGGGGC NGGGGGGGGG GGGGGNNNNN 721 NNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl