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pcDNA3.1 FLAG-hRFWD3 C315A

Expression vector of human ring finger and WD repeat domain 3 (RFWD3) mutant, C315A, RING finger domain mutation.

Catalog number RDB17491
Resource name pcDNA3.1 FLAG-hRFWD3 C315A
Clone info. Expression vector of human ring finger and WD repeat domain 3 (RFWD3) mutant, C315A, RING finger domain mutation, tagged with FLAG at N-terminus.
Comment Expression was confirmed by the depositor with immunoblotting. Known differences (at least): G to A substitution at 1269nt of NM_018124.4 (R392K; AGG to AAG).
Vector backbone pcDNA3.1/Zeo (plasmid)
Selectable markers E. coli, Ampicillin; mammalian cells, Zeocin.
Gene/insert name Human RFWD3 cDNA
Depositor|Developer Takata, Minoru |
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External Database
Human RFWD3

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Inano, S et al. Mol. Cell, 66(5): 622-634, 2017)
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          Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Inano, S et al. Mol. Cell, 66(5): 622-634, 2017)。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
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          RDB17491 pcDNA3.1 FLAG-hRFWD3 C315A DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Inano, S., RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol. Cell 66 (5): 622-634.e8 (2017). PMID 28575658.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB17491_A9Iop1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro, FLAG, attB1, insert 5'
          Sequence file: RDB17491_A9Ioa.seq check
          >D06551A2_A9Io_2_CMV_Forward_D01_10_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNTGGGCGG TAGGCGTNNN NANNNNNNGA
             61 GGGTCTATAT AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG
            121 AAATTAATAC GACTCACTAT AGGGAGACCC AAGCTGGCTA GCACCATGGA TTATAAAGAT
            181 GATGACGATA AGCATGGTGG TTCTGGTCCA AGCTATCAAA CAAGTTTGTA CAAAAAAGCA
            241 GGCTCCGCGG CCGCCCCCTT CACCATGGCT CATGAAGCAA TGGAATATGA TGTTCAGGTG
            301 CAGTTAAATC ATGCCGAACA ACAGCCAGCT CCTGCTGGCA TGGCCAGCAG CCAAGGGGGA
            361 CCAGCCCTCC TCCAGCCTGT TCCTGCTGAT GTGGTCAGCA GCCAGGGGGT ACCATCCATC
            421 CTCCAGCCAG CTCCTGCTGA GGTGATCAGC AGCCAAGCGA CACCACCCCT GCTCCAGCCT
            481 GCTCCGCAAC TGTCTGTTGA CCTGACAGAA GTGGAGGTCT TGGGAGAAGA CACTGTGGAG
            541 AACATCAATC CAAGAACTTC AGAACAACAT AGGCAGGGAT CTGATGGTAA TCACACCATC
            601 CCAGCATCTT CGTTGCATTC AATGACCAAC TTCATCAGCG GACTGCAGAG ACTTCATGGC
            661 ATGCTGGAAT TCCTGAGACC TTCATCTTCA AACCACAGTG TAGGGCCAAT GAGAACAAGA
            721 AGGAGGGTAT NNNNNNCACG GAGGGCAAGA GCCGGANGGT CTCAGAGGAC AGACAGTGCC
            781 AGGTTGANAG CACCATTGGA TGCTTACTTT CANGTGAGCA GGANCCAGCC TGANTTGCCA
            841 GCTACCACTT ATGATTCAGA NNNTAGGAAT CCTGTATCTG AAGANTTGCA GGTGTCTANT
            901 AGTTCTGATT CTGACAGNGA CAGCTCTGCN NANNATGGAN GGGTTGTTGN NCAGNCANAN
            961 GNNNNTGGAN CTGTCNTTTT AGNANNNNNN NNCTAGCNNG TGTNNNAGCN NNNCNANNAN
          1021 NNTNACNTGT ATNGATGGAN NNNNNNNNCC TNNNNNNANC NNNNNNNNNN
          //
          Primer: BGH_rev2
          Region: bGHp(A), attB2, insert 3'
          Sequence file: RDB17491_A9Iob.seq check
          >D06551A2_A9Io_2_BGH_rev2_E01_13_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGNN NNGGNNAACN ACNGATGGCT GGNNNNNAGA
             61 AGGCACAGTC GAGGCTGATC AGCGGGTTTA TCGAACCACT TTGTACAAGA AAGCTGGGTC
            121 GGCGCGCCCA CCCTTTCACT CCCACTTATA GATGTGGACC ATCTTCTCTG TTAAGGTAGC
            181 CAAGTAGCTG TTACGGTTCA CCTCAAATGG GCAGATGTCC AACACAGGCT GATCGGTCTG
            241 TAGGTCCTGG AGCAACGAGC CACTGGCAGC ATCCCACAGC AGGGCAGAAT TTGCTGCTTC
            301 ATCCCCAGTA CACACCAGGA TGTTGCCATC ATTCTCTGGG CTTTGGAAAA TGGCATTTTT
            361 GGTCAATAGT TTGCAAGTAG GTCCTCCAAA AAATGTATGT NNNNNNNGGC AGGAGCAGAT
            421 TGGATTTCCA GTGTCATCCA GTCGGTAGGA CATTTCCATC AGCACACTTC GTATGGTGGT
            481 GTGATTTTTA TCAGGCCTGT AGGTCACAAG ACAGTGCCGG GAGCTGTTCT CTGTCTGAAA
            541 GTCTATGCAG CCCCCTGGCT CCAAGGGCAG CACATGAGGC CAATGAGAAA AGTCCATTTT
            601 CTGTTCCCAG AATGAAGCAT CCTCCAAGGT TCCAGCCAGC ACCCCACCAT ATGGAAATGC
            661 AGCTGAGGCA GCTCTGGGCA TGTATNANNN GGAGACCAGT GGGCATCTGG CTTTCTGAGC
            721 TACTAACTCC TGCACATGAC TGCTCGTGTT TCGCACGTCA TATACCAGAA TTGAACCATT
            781 GGCCAGTCCA GCATAGATGT AGTTAGCCTC ATCAAGACAC CAGCAACAGC TCCNGANAGG
            841 ACGTCCAGCA TTATNAGTCT GGANNNCNGT ATTTGTCTNN NGGCNGGNCA GTTNAATAGT
            901 GTTGTCTAGG GAANCANANA GTANCAAGCN TCTNANGNAA CTGCTAAACG CCNNNNCNCN
            961 GNTCTGTTTG NNNTGCATCN GNAANGTACN GACTGNTNTN NNNGTTGGCN NTNNNCNNCA
          1021 TNNNANNNCN NANNNCNNNN NNNNAAAAGA GGNCNNGNNN NANNANNNNN NNNNN
          //
          Primer: hRFWD3-N_F
          Region: insert mid (C315A)
          Sequence file: RDB17491_A9Ioc.seq check
          >D06551A2_A9Io_2_hRFWD3-N_F_A01_01_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNTCTGAANA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA
             61 CAGTGACAGC TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT
            121 CATTTTAGAA GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG
            181 AGGCAAGACC CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC
            241 TTCTATGGAT GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC
            301 TGGGGACCAC CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGGCCATTTC
            361 CACGTGGCTT AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG
            421 TGACATTGTC GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG
            481 CATGAAAAGT TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC
            541 ACAGTGCCGA CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAAGCTTC AAAGGCGTGT
            601 TCAGGACTTG CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC
            661 CCAAGCATGG GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA
            721 CTTCCAAAAG ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA
            781 TGCTCTGAGC TGCCTGGTGA TATCACAGCC TTCTCCTCAN GCCTCTTTTC TTCCAGGCTT
            841 TNGTGTTAAG ATGTTGAGTA CTGCCAACAT GAANAGCANT CAGTACATTC CGATGCATGN
            901 CAAACAGATN CGNNNANNNN NGTTTANCAG TTANCTCANA NGCTTGCTNC TCTCTGCTTN
          961 CCNANACNAC ACTATTAAAC TGNNNNGCNN NNNNNNNN
          //
          Primer: SV40pro_ori_F
          Region: SV40pro_ori, bleoR, SV40 p(A)
          Sequence file: RDB17491_A9Iod.seq check
          >D06551A2_A9Io_2_SV40pro_ori_F_F01_16_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNCNN NNNNNNNNCT CTGCCTCTGN NCTATTCNNN ANNNNNGTGA
             61 GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT
            121 TTCGGATCTG ATCAGCACGT GTTGACAATT AATCATCGGC ATAGTATATC GGCATAGTAT
            181 AATACGACAA GGTGAGGAAC TAAACCATGG CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA
            241 CCGCGCGCGA CGTCGCCGGA GCGGTCGAGT TCTGGACCGA CCGGCTCGGG TTCTCCCGGG
            301 ACTTCGTGGA GGACGACTTC GCCGGTGTGG TCCGGGACGA CGTGACCCTG TTCATCAGCG
            361 CGGTCCAGGA CCAGGTGGTG CCGGACAACA CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG
            421 ACGAGCTGTA CGCCGAGTGG TCGGAGGTCG TGTCCACGAA CTTCCGGGAC GCCTCCGGGC
            481 CGGCCATGAC CGAGATCGGC GAGCAGCCGT GGGGGCGGGA GTTCGCCCTG CGCGACCCGG
            541 CCGGCAACTG CGTGCACTTC GTGGCCGAGG AGCAGGACTG ACACGTGCTA CGAGATTTCG
            601 ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT
            661 GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA
            721 TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT
            781 TTTTTTCACT GCATTCNAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT
            841 GTATACCGTC GANCTCTAGC TNGAGCTTNN CGTAATCATG GTCATAGCTG TTNNCTGTGT
            901 GAAATTGNTA TNCNGCTCAC AATTCNNCAC AANATACGAN NCNGNAANNC NTAAAGNGTA
            961 ANNCCTGGGG TNNCNAATGA GTGANCTAAC TCACNTTNAN TTGNNGTTGC GNNCNCNGNC
          1021 CNNCTTTNNN NNNNNGGNNA NNNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Inano, S., RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol. Cell 66 (5): 622-634.e8 (2017). PMID 28575658.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl