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pcDNA3.1 FLAG-hRFWD3 S46/63A

Expression vector of human ring finger and WD repeat domain 3 (RFWD3) mutant, S46/63A, non-phosphorylatable mutation.

Catalog number RDB17493
Resource name pcDNA3.1 FLAG-hRFWD3 S46/63A
Clone info. Expression vector of human ring finger and WD repeat domain 3 (RFWD3) mutant, S46/63A, non-phosphorylatable mutation, tagged with FLAG at N-terminus.
Comment Expression was confirmed by the depositor with immunoblotting. Known differences (at least): G to A substitution at 1269nt of NM_018124.4 (R392K; AGG to AAG).
Vector backbone pcDNA3.1/Zeo (plasmid)
Selectable markers E. coli, Ampicillin; mammalian cells, Zeocin.
Gene/insert name Human RFWD3 cDNA
Depositor|Developer Takata, Minoru |
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External Database
Human RFWD3

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Inano, S et al. Mol. Cell, 66(5): 622-634, 2017)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Inano, S et al. Mol. Cell, 66(5): 622-634, 2017)。
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          RDB17493 pcDNA3.1 FLAG-hRFWD3 S46/63A DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Inano, S., RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol. Cell 66 (5): 622-634.e8 (2017). PMID 28575658.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB17493_A9Iop1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro, attB1, FLAG, insert 5' (S46A, S63A)
          Sequence file: RDB17493_A9Ioa.seq check
          >D06551A4_A9Io_1_CMV_Forward_B02_05_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNTG NNGCNNATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
             61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA
            121 CGACTCACTA TAGGGAGACC CAAGCTGGCT AGCACCATGG ATTATAAAGA TGATGACGAT
            181 AAGCATGGTG GTTCTGGTCC AAGCTATCAA ACAAGTTTGT ACAAAAAAGC AGGCTCCGCG
            241 GCCGCCCCCT TCACCATGGC TCATGAAGCA ATGGAATATG ATGTTCAGGT GCAGTTAAAT
            301 CATGCCGAAC AACAGCCAGC TCCTGCTGGC ATGGCCAGCA GCCAAGGGGG ACCAGCCCTC
            361 CTCCAGCCTG TTCCTGCTGA TGTGGTCAGC GCCCAGGGGG TACCATCCAT CCTCCAGCCA
            421 GCTCCTGCTG AGGTGATCAG CGCCCAAGCG ACACCACCCC TGCTCCAGCC TGCTCCGCAA
            481 CTGTCTGTTG ACCTGACAGA AGTGGAGGTC TTGGGAGAAG ACACTGTGGA GAACATCAAT
            541 CCAAGAACTT CAGAACAACA TAGGCAGGGA TCTGATGGTA ATCACACCAT CCCAGCATCT
            601 TCGTTGCATT CAATGACCAA CTTCATCAGC GGACTGCAGA GACTTCATGG CATGCTGGAA
            661 TTCCTGAGAC CTTCATCTTC AAACCACAGT GTAGGGCCAA TGAGAACAAG AAGGAGGGTA
            721 TCTGCTTCAC GGAGGGCAAG AGCCGGANGG TCTCAGAGGA NAGACAGTGC CAGGTTGAGA
            781 GCACCATTGG ATGCTTACTT TCAGGTGAGC AGGANCCAGC CTGACTTGCC NGCTACCACT
            841 TATGATTCAN AGACTANGAA TCCTGTATCT GAAGANTTGC ANGTGTCTAG TAGTTCTGAT
            901 TCTGANANTG NNAGCTCTGC ANANTATGGA NGGGTTGNTG NNCNNGCANA NGNNNCTGGA
          961 NCTGNCATTT TANANNANCN NNNANCNNNN GNNNNANCAN ANCNNNNNNN NNN
          //
          Primer: BGH_rev2
          Region: bGH p(A), attB2, insert 3'
          Sequence file: RDB17493_A9Iob.seq check
          >D06551A4_A9Io_1_BGH_rev2_C02_08_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNG GNGGNNGGGC AAACNACAGA TGGCTGGCAA CTAGAAGGCA
             61 CAGTCGAGGC TGATCAGCGG GTTTATCGAA CCACTTTGTA CAAGAAAGCT GGGTCGGCGC
            121 GCCCACCCTT TCACTCCCAC TTATAGATGT GGACCATCTT CTCTGTTAAG GTAGCCAAGT
            181 AGCTGTTACG GTTCACCTCA AATGGGCAGA TGTCCAACAC AGGCTGATCG GTCTGTAGGT
            241 CCTGGAGCAA CGAGCCACTG GCAGCATCCC ACAGCAGGGC AGAATTTGCT GCTTCATCCC
            301 CAGTACACAC CAGGATGTTG CCATCATTCT CTGGGCTTTG GAAAATGGCA TTTTTGGTCA
            361 ATAGTTTGCA AGTAGGTCCT CCAAAAAATG TATGTACAGG CTGGCAGGAG CAGATTGGAT
            421 TTCCAGTGTC ATCCAGTCGG TAGGACATTT CCATCAGCAC ACTTCGTATG GTGGTGTGAT
            481 TTTTATCAGG CCTGTAGGTC ACAAGACAGT GCCGGGAGCT GTTCTCTGTC TGAAAGTCTA
            541 TGCAGCCCCC TGGCTCCAAG GGCAGCACAT GAGGCCAATG AGAAAAGTCC ATTTTCTGTT
            601 CCCAGAATGA AGCATCCTCC AAGGTTCCAG CCAGCACCCC ACCATATGGA AATGCAGCTG
            661 AGGCAGCTCT GGGCATGTAT GACAGGGAGA NCAGTGGGCA TCTGGCTTTC TGAGCTACTA
            721 ACTCCTGCAC ATGACTGCTC GTGTTTCGCA CGTCATATAC CAGAATTGAA CCATTGGCCA
            781 GTCCAGCATA GATGTAGTTA GCCTCATCAA GACACCAGCA ACAGCTCCAG ACAGGANGTC
            841 CAGCATTATA AGTCTGGANC ACGGNATTTG TCTNCNGGCT GGTCAGTTTA ATAGTGNTGN
            901 CTANGGAAGC AGANAGTANC AAGCCTCTGA NNTNNCTGNT AAACGCCAGN NNNCGGATCN
          961 GNTNGNNNTG CATCNGANNG TACTGACNGN TCNTCNTGNN NNNNNNNN
          //
          Primer: hRFWD3-N_F
          Region: insert mid
          Sequence file: RDB17493_A9Ioc.seq check
          >D06551A4_A9Io_1_hRFWD3-N_F_D04_10_ABI24.ab1
              1 NNNNNNNNNN NNNNNCNNNN ATCTGNNNNN TGCAGGTGTC TAGTAGTTCT GATTCTGACA
             61 GTGACAGCTC TGCAGAGTAT GGAGGGGTTG TTGACCAGGC AGAGGAATCT GGAGCTGTCA
            121 TTTTAGAAGA GCAACTAGCA GGTGTCTCAG CAGAGCAAGA AGTTACATGT ATCGATGGAG
            181 GCAAGACCCT CCCCAAACAG CCATCTCCCC AGAAGTCTGA GCCTCTGCTA CCTTCTGCTT
            241 CTATGGATGA GGAAGAAGGG GACACTTGTA CAATATGTCT GGAACAGTGG ACCAATGCTG
            301 GGGACCACCG GCTCTCAGCA TTACGCTGTG GGCATCTCTT TGGGTATAGG TGCATTTCCA
            361 CGTGGCTTAA AGGACAAGTA CGAAAATGTC CCCAGTGCAA CAAGAAAGCC AGGCACAGTG
            421 ACATTGTCGT CCTTTATGCC CGAACCCTGA GAGCTTTGGA CACTAGTGAA CAGGAGCGCA
            481 TGAAAAGTTC CCTACTGAAG GAACAGATGC TAAGGAAACA GGCCGAGTTA GAATCAGCAC
            541 AGTGCCGACT CCAACTGCAG GTCCTCACTG ATAAGTGCAC TAAGCTTCAA AGGCGTGTTC
            601 AGGACTTGCA AAAACTTACG TCACATCAAA GTCAGAATTT ACAGCAACCC AGGGGCTCCC
            661 AAGCATGGGT CCTGAGCTGC TCACCCTCCA GCCAGGGCCA GCACAAGCAC AAGTACCACT
            721 TCCAAAAGAC CTTCACAGTA TCTCAGGCAG GAAACTGCCG GATCATGGCA TACTGTGATG
            781 CTCTGAGCTG CCTGGTGATA TCACAGCCTT CTCCTCANGC CTCTTTNCTT CCAGGCTTTG
            841 GTGTTAAGAT GTTGAGTANT GCCAACATGA AGAGCAGTCN GTACATTCCG ATGCATGGCA
            901 AACAGATCCG NGNANTGNCG TTNANCAGTT ACCTCANANG CTTGCTACTC TNNGCTNNCN
            961 NANANNNCAC NATTAANNTG ANNAGCCNNN NNNNNAATAC CGNNGNNNNN ANNTATNNNG
          1021 NNNNNNNNNN
          //
          Primer: SV40pro_ori_F
          Region: SV40 pro_ori, bleoR
          Sequence file: RDB17493_A9Iod.seq check
          >D06551A4_A9Io_1_SV40pro_ori_F_D02_11_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN TGCNTCTGAG CTNTTCNNGA AGTAGTGAGG
             61 AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT
            121 CGGATCTGAT CAGCACGTGT TGACAATTAA TCATCGGCAT AGTATATCGG CATAGTATAA
            181 TACGACAAGG TGAGGAACTA AACCATGGCC AAGTTGACCA GTGCCGTTCC GGTGCTCACC
            241 GCGCGCGACG TCGCCGGAGC GGTCGAGTTC TGGACCGACC GGCTCGGGTT CTCCCGGGAC
            301 TTCGTGGAGG ACGACTTCGC CGGTGTGGTC CGGGACGACG TGACCCTGTT CATCAGCGCG
            361 GTCCAGGACC AGGTGGTGCC GGACAACACC CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC
            421 GAGCTGTACG CCGAGTGGTC GGAGGTCGTG TCCACGAACT TCCGGGACGC CTCCGGGCCG
            481 GCCATGACCG AGATCGGCGA GCAGCCGTGG GGGCGGGAGT TCGCCCTGCG CGACCCGGCC
            541 GGCAACTGCG TGCACTTCGT GGCCGAGGAG CANGACTGAC ACGTGCTACG AGATTTCGAT
            601 TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG
            661 ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTTGTTTATT
            721 GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT
            781 TTTTCACTGC ATTCTAGTTG TGGTTTGTCC NAACTCATCN ATGTATCTTA TCATGTCTGT
            841 ATACCGTCGA CCTCTANCTA GAGCTTGGCG TANNCATGGT CATAGCTGTT TCCTGTGTGA
            901 AATTGTTANC CGCTCACANT TNCNCACAAC NTACGANCCN GNANNCNNNA NGTGNNNAAG
            961 CCNGGGGGTG NCNNNNNGAG TGAGCNNACT CACATTNANT TGCNNTTGCN CTNNNNNNCC
          1021 NNNTTTNNNG NNNNGGGAAA NNNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Inano, S., RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol. Cell 66 (5): 622-634.e8 (2017). PMID 28575658.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl