Please review the QC test results indicated by icon below as well as clone information before placing your order.

CSII FLAG-hRFWD3 wt

Lentivirus expression vector of human ring finger and WD repeat domain 3 (RFWD3) wild type.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Inano, S et al. Mol. Cell, 66(5): 622-634, 2017)
Additional terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Ordering	Please visit Ordering instruction.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Remarks	Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Inano, S et al. Mol. Cell, 66(5): 622-634, 2017)。
付加的提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。 提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
備考	Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17496_A9Iop1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: hRFWD3-N_R Region: insert 5', attB1, FLAG, CMV pro 2, CMV en 2 Sequence file: RDB17496_A9Ioa.seq
>D06551A7_A9Io_2_hRFWD3-N_R_G02_20_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN TNNGCAGGNN CTGGCTGGAN GNNGGATGGT    61 ACCCCCTGGC TGCTGACCAC ATCAGCAGGA ACAGGCTGGA GGAGGGCTGG TCCCCCTTGG   121 CTGCTGGCCA TGCCAGCAGG AGCTGGCTGT TGTTCGGCAT GATTTAACTG CACCTGAACA   181 TCATATTCCA TTGCTTCATG AGCCATGGTG AAGGGGGCGG CCGCGGAGCC TGCTTTTTTG   241 TACAAACTTG TTTGATAGCT TGGACCAGAA CCACCATGCT TATCGTCATC ATCTTTATAA   301 TCCATGGTGC TAGCCAGCTT GGGTCTCCCT AGCGGATCTG ACGGTTCACT AAACCAGCTC   361 TGCTTATATA GACCTCCCAC CGTACACGCC TACCGCCCAT TTGCGTCAAT GGGGCGGAGT   421 TGTTACGACA TTTTGGAAAG TCCCGTTGAT TTTGGTGCCA AAACAAACTC CCATTGACGT   481 CAATGGGGTG GAGACTTGGA AATCCCCGTG AGTCAAACCG CTATCCACGC CCATTGATGT   541 ACTGCCAAAA CCGCATCACC ATGGTAATAG CGATGACTAA TACGTAGATG TACTGCCAAG   601 TAGGAAAGTC CCATAAGGTC ATGTACTGGG CATAATGCCA GGCGGGCCAT TTACCGTCAT   661 TGACGTCAAT AGGGGGCGTA CTTGGCATAT GATACACTTG ATGTACTGCC AAGTGGGCAG   721 TTTACCGTAA ATACTCCACC CATTGACGTC AATGGAAAGT CCCTATTGGC GTTACTATGG   781 GAACATACGT CATTATTGAC GTCAATGGGC GGGGGTCGTT GGGCGGTCAG CCAGGCGGGC   841 CATTTACCGT AAGTTATGTA ACGCGGANNT CCNTATATGG GCTATGAACT AATGACCCCG   901 TAATTGATTA CTATTAATCG AATTANNTCN AAGTGAATTT CTGCTGNCCN NGNNATAAAC   961 CCNAAAATTT TGAATTTTTT GTNATTTGTT TTTGTAANTN CNTTANNTTG NATGNNNGNT 1021 NGNNNNNNNG NNNNNNANNN NTNNCCNNNN NNNNNN //
Primer: IRES_R3 Region: attB2, insert 3' Sequence file: RDB17496_A9Iob.seq
>D06551A7_A9Io_2_IRES_R3_F03_18_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNCNNN NNNNGCGGNN NNNNGCAGAA    61 TTCTCGATGG CAACTAGAAG GCACAGTCGA GGCTGATCAG CGGGTTTATC GAACCACTTT   121 GTACNNNAAA GCTGGGTCGG CGCGCCCACC CTTTCACTCC CACTTATANA TGTGGACCAT   181 CTTCTCTGTT AAGGTAGCCA AGTAGCTGTT ACGGTTCACC TCAAATGGGC AGATGTCCAA   241 CACAGGCTGA TCGGTCTGTA GGTCCTGGAG CAACGAGCCA CTGGCAGCAT CCCACAGCAG   301 GGCAGAATTT GCTGCTTCAT CCCCAGTACA CACCAGGATG TTGCCATCAT TCTCTGGGCT   361 TTGGAAAATG GCATTTTTGG TCAATAGTTT GCAAGTAGGT CCTCCAAAAA ATGTATGTAC   421 NGGCTGGCAG GAGCAGATTG GATTTCCAGT GTCATCCAGT CGGTAGGACA TTTCCATCAN   481 CACACTTCGT ATGGTGGTGT GATTTTTATC AGGCCTGTAG GTCACAAGAC NNNGCCGGGA   541 GCTGTTCTCT GTCTGAAAGT CTATGCAGCC CCCTGGCTCC AAGGGCAGNN NNNGAGGCCA   601 ATGAGAAAAG TCCATTTTCT GTTCCCANAA TGAAGCATCC TNNNNNGTTC CAGCCAGCAC   661 CNNNNNNTAT GNANNTGCNN CTGANGCAGC TCTGGGCATG TATGACAGGN ANACCAGTGG   721 GCATCTGGCT TTCTGAGCTA CTAACTCCTG CACATGACTG CTCGTGTTTC GCACGTCATN   781 NTACNNNAAN TTGAACCATT GGCCANNCCN NCATAGATGT AGTTNAGCCT CATCAAGACA   841 CCAGCAACAG CTNNNGANNG GNANNNNNNN NCNTTATNAG TCTGGGANNN CNNGNNNTTT   901 GNNTCCAGGC TGGNNCNNNT TTNANNANNN NNTGNCTAGG GAAANCANAA NAGTANNNNN   961 CCTCTGANGN NANNNNNNNN AANNNNNNNT NCNNNGGATN NNNNNNGNCN NNNNNNCGGA 1021 ANNGNNNNNN NNNN //
Primer: hRFWD3-N_F Region: insert mid Sequence file: RDB17496_A9Ioc.seq
>D06551A7_A9Io_2_hRFWD3-N_F_C01_07_ABI24.ab1     1 NNNNNNNNNN NGNNNNNNNN NTCTGANAGT TGCAGGTGTC TAGTAGTTCT GATTCTGACA    61 GTGACAGCTC TGCAGAGTAT GGAGGGGTTG TTGACCAGGC AGAGGAATCT GGAGCTGTCA   121 TTTTAGAAGA GCAACTAGCA GGTGTCTCAG CAGAGCAAGA AGTTACATGT ATCGATGGAG   181 GCAAGACCCT CCCCAAACAG CCATCTCCCC AGAAGTCTGA GCCTCTGCTA CCTTCTGCTT   241 CTATGGATGA GGAAGAAGGG GACACTTGTA CAATATGTCT GGAACAGTGG ACCAATGCTG   301 GGGACCACCG GCTCTCAGCA TTACGCTGTG GGCATCTCTT TGGGTATAGG TGCATTTCCA   361 CGTGGCTTAA AGGACAAGTA CGAAAATGTC CCCAGTGCAA CAAGAAAGCC AGGCACAGTG   421 ACATTGTCGT CCTTTATGCC CGAACCCTGA GAGCTTTGGA CACTAGTGAA CAGGAGCGCA   481 TGAAAAGTTC CCTACTGAAG GAACAGATGC TAAGGAAACA GGCCGAGTTA GAATCAGCAC   541 AGTGCCGACT CCAACTGCAG GTCCTCACTG ATAAGTGCAC TAAGCTTCAA AGGCGTGTTC   601 AGGACTTGCA AAAACTTACG TCACATCAAA GTCAGAATTT ACAGCAACCC AGGGGCTCCC   661 AAGCATGGGT CCTGAGCTGC TCACCCTCCA GCCAGGGCCA GCACAAGCAC AAGTACCACT   721 TCCAAAAGAN CTTCACAGTA TCTCAGGCAG GAAACTGCCG GATCATGGCA TACTGTGATG   781 CTCTGANCTG CCTGGTGATA TCACAGCCTT CTCCTCANGC CTCTTTTCTT CCAGGCTTTG   841 GTGTTAAGAN GTTGANTACT GCCAACATGA AGAGCAGTCA GTACATTCCG ATGCATGGCA   901 AACAGATNCG NGGANNNGCG TTTANCAGTT ANNNCANANG CTNGCTACTC TCTGCTTCCN   961 NNGANANCNC TATNNAANNG ANNAGCCNNN NNANNAATNN CNNNNNNCNN ANNTNATNAN 1021 GNTGNNNNNN NNNN //
Primer: HIVgp_R1 Region: HIV-1 psi, 5' LTR Sequence file: RDB17496_A9Iod.seq
>D06551A7_A9Io_2_HIVgp_R1_C01_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNGC TCTTTTTTCT CTCTGCACCN CTCTTCTCTT TGCCTTGGTG    61 GGTGCTACTC CTAATGGTTC AATTTTTACT ACTTTATATT TATATAATTC ACTTCTCCAA   121 TTGTCCCTCA TATCTCCTCC TCCAGGTCTG AAGATCAGCG GCCGGCCGCT TGCTGTGCGG   181 TGGTCTTACT TTTGTTTTGC TCTTCCTCTA TCTTGTCTAA AGCTTCCTTG GTGTCTTTTA   241 TCTCTATCCT TTGATGCACA CAATAGAGGG TTGCTACTGT ATTATATAAT GATCTAAGTT   301 CTTCTGATCC TGTCTGAAGG GATGGTTGTA GCTGTCCCAG TATTTGTCTA CAGCCTTCTG   361 ATGTTTCTAA CAGGCCAGGA TTAACTGCGA ATCGTTCTAG CTCCCTGCTT GCCCATACTA   421 TATGTTTTAA TTTATATTTT TTCTTTCCCC CTGGCCTTAA CCGAATTTTT TCCCATCGCG   481 ATCTAATTCT CCCCCGCTTA ATACTGACGC TCTCGCACCC ATCTCTCTCC TTCTAGCCTC   541 CGCTAGTCAA AATTTTTGGC GTACTCACCA GTCGCCGCCC CTCGCCTCTT GCCGTGCGCG   601 CTTCAGCAAG CCGAGTCCTG CGTCGAGAGA GCTCCTCTGG TTTCCCTTTC GCTTTCAAGT   661 CCCTGTTCGG GCGCCACTGC TAGAGATTTT CCACACTGAC TAAAAGGGTC TGANGGATCT   721 CTAGTTACCA GAGTCACACA ACAGACGGGC ACACACTACT TGAAGCACTC AAGGCAAGCT   781 TTATTGAGGC TTAAGCAGTG GGTTCCCTAG TTAGCCAGAG AGCTCCCAGG CTCAGATCTG   841 GTCTAACCAG ANAGACCCAG TACAGGCAAA ANGCGCTGCT TATATAGANN TCCCACCGNA   901 CACGCCTANC GCCCATTTGC GNNAATGGGG CGGANTNGTT NCNANNTTTT GNAAAGTCCC 961 GTTGANTTTG GTGNCAAANN AAANNNCCNT TGANNNNNNN GGGGNNNNNN NNNN //
Primer: cPPT+CTS_v2_F Region: cPPT_CTS, CMV en 2, CMV pro 2 Sequence file: RDB17496_A9Ioe.seq
>D06551A7_A9Io_2_cPPT+CTS_v2_F_D01_10_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNACNA NNACNAATTA CAAAAATTCA AAATTTTCGG    61 GTTTATTACA GGGACAGCAG AAATTCACTT TGAATTAATT CGATTAATAG TAATCAATTA   121 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG   181 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC   241 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA   301 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA   361 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA   421 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT   481 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG   541 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA   601 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA   661 GAGCTGGTTT AGTGAACCGT CNNATCCGCT AGGGAGACCC AAGCTGGCTA GCACCATGGA   721 TTATAAAGAT GATGACGATA AGCATGGTGG TTCTGGTCCA AGCTATCAAA CAAGTTTGTA   781 CAAAAAAGCA NGCTCCGCGG CCGCCCCCTT CACNATGGCT CATGAANCNN NGNAATATGA   841 NGTTCANGTG CAGTTAANTC ATGCCNAACA ACANCCAGCT NCTGCTGGCA NGGNCAGCAG   901 CNNNGGGGGN NCANCCCTCC TNCANCCTGN NNCNGNTGAT GTGNNCAGCA GCCAGGGGGN   961 ACNNTNNNTC CNNNNNCNNN NNNNNGCNGN NNNATNANCA GNCNNANCGN NANNACCNNN 1021 NNNNNNN //
Primer: IRES-F Region: IRES2, BSD, WPRE Sequence file: RDB17496_A9Iof.seq
>D06551A7_A9Io_2_IRES-F_F01_16_ABI24.ab1     1 NNNNNNNNNN NNNNNNNTNN GTCNANGTTN NAAAAACGTC TAGGCCCCCC GAACCACGGG    61 GACGTGGTTT TCCTTTGAAA AACACGATGA TAATATGGCC ACAACCATGG CCAAGCCTTT   121 GTCTCAAGAA GAATCCACCC TCATTGAAAG AGCAACGGCT ACAATCAACA GCATCCCCAT   181 CTCTGAAGAC TACAGCGTCG CCAGCGCAGC TCTCTCTAGC GACGGCCGCA TCTTCACTGG   241 TGTCAATGTA TATCATTTTA CTGGGGGACC TTGTGCAGAA CTCGTGGTGC TGGGCACTGC   301 TGCTGCTGCG GCAGCTGGCA ACCTGACTTG TATCGTCGCG ATCGGAAATG AGAACAGGGG   361 CATCTTGAGC CCCTGCGGAC GGTGCCGACA GGTGCTTCTC GATCTGCATC CTGGGATCAA   421 AGCCATAGTG AAGGACAGTG ATGGACAGCC GACGGCAGTT GGGATTCGTG AATTGCTGCC   481 CTCTGGTTAT GTGTGGGAGG GCTAAGTCGA GCCTCTAGAG TTAACATCGA GGGATCAAGC   541 TTATCGATAA TCAACCTCTG GATTACAAAA TTTGTGAAAG ATTGACTGGT ATTCTTAACT   601 ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT GCCTTTGTAT CATGCTATTG   661 CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC CTGGTTGCTG TCTCTTTATG   721 AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG CACTGTGTTT GCTGACGCAA   781 CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT TTCCGGGANT TTCGCTTTCC   841 CCCTNNNTAT TGCCACGGCG GAACTCATCG CCGCCTGCNT TGNCCGCTGC TGGANAGGGG   901 CTCGGCTGTT NGGCACTGAC NATNNCGNNN NGNTGTCGGG GAAGCTGANG TCCTTTCCAN   961 GGNTGCTCNN CTGNGNNGNC NCCNNNNNTC TGNGCGGGNN GNCCNTNNNG NNNCNNNCNT 1021 NNNGNNCNNN NNTNNNNCNN ANNNTNNNNT NNNNNNNGNN NNNNNNNN //
Primer: BGH_rev2 Region: bGH p(A), 3' LTR, WPRE Sequence file: RDB17496_A9Iog.seq
>D06551A7_A9Io_2_BGH_rev2_E01_13_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNG GGGGNNGGGC AAACNACAGA TGGCTGGCAA CTAGAAGGCA    61 CAGTCGAGGC TGATCAGCGG GTTTAAACGG GCCCTGCTAG AGATTTTCCA CACTGACTAA   121 AAGGGTCTGA GGGATCTCTA GTTACCAGAG TCACACAACA GACGGGCACA CACTACTTGA   181 AGCACTCAAG GCAAGCTTTA TTGAGGCTTA AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC   241 TCCCAGGCTC AGATCTGGTC TAACCAGAGA GACCCAGTAC AGTCCGGATG CAGCTCTCGG   301 GCCATGTGAT GAAATGCTAG GCGGCTGTCA AACCTCCACT CTAATACTTC TCTCTCCGGG   361 TCATCCATCC CATGCAGGCT CACAGGGTGT AACAAGCGGG TGTTCTCTCC TTCATTGGCT   421 TCTTCTACCT TCTCTTGCTC AACTGGTACT AGCTTGTAGC ACCATCCAAA GGTCAGTGGA   481 TATCTGATCC CTGGCCCTGG TGTGTAGTTC TGCCAATCAG GGAAGTAGCC TTGTGTGTGG   541 TAGATCCACA GATCAAGGAT ATCTTGTCTT CGTTGGGAGT GAATTAGCCC TTCCAGTCCC   601 CCCTTTTCTT TTAAAAAGTG GCTAAGATCT ACAGCTGCCT TGTAAGTCAT TGGTCTTAAA   661 GGTACCTGAG GTGTGACTGG AAAACCCACC TCCTCCTCCT CTTGTGCTTC TAGCCAGGCA   721 CAATCAGCAT TGGTAGCTGC TGTATTGCTA CTTGTGATTG CTCCATGTTT TTCTAGGTCT   781 CGACGGTATC GATGCGGGGA NGCGGCCCAA AGGGAGATCC GANTCGTCTG ANGGCGAANG   841 CGAAGANGCG GAAGANGNCG CAGANCNNGN AGCANGNCGC GGGAANGAAN GTCCGCTNGA   901 NTGAGGGNCG AANGGANGTA NCANANNNNA NGTCCCGCGC ANAATCCNNN TGGNNNCNCA   961 GGNGANCANC CANNGNAANN NNNGNCANCT TNCNNGANNN NNNNNNGNNN NNGNCNNNNN 1021 NNNNN //
Primer: SV40pro_ori_F Region: SV40 pro_ori, EM7 pro, BleoR Sequence file: RDB17496_A9Ioh.seq
>D06551A7_A9Io_2_SV40pro_ori_F_G01_19_ABI24.ab1     1 NNNNNNNNNN NNNGNNGNCN CCTCTGCCTC TGANCTATTC NNGAAGTAGT GAGGAGGCTT    61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC   121 TGATCAGCAC GTGTTGACAA TTAATCATCG GCATAGTATA TCGGCATAGT ATAATACGAC   181 AAGGTGAGGA ACTAAACCAT GGCCAAGTTG ACCAGTGCCG TTCCGGTGCT CACCGCGCGC   241 GACGTCGCCG GAGCGGTCGA GTTCTGGACC GACCGGCTCG GGTTCTCCCG GGACTTCGTG   301 GAGGACGACT TCGCCGGTGT GGTCCGGGAC GACGTGACCC TGTTCATCAG CGCGGTCCAG   361 GACCAGGTGG TGCCGGACAA CACCCTGGCC TGGGTGTGGG TGCGCGGCCT GGACGAGCTG   421 TACGCCGAGT GGTCGGAGGT CGTGTCCACG AACTTCCGGG ACGCCTCCGG GCCGGCCATG   481 ACCGAGATCG GCGAGCAGCC GTGGGGGCGG GAGTTCGCCC TGCGCGACCC GGCCGGCAAC   541 TGCGTGCACT TCGTGGCCGA GGAGCAGGAC TGACACGTGC TACGAGATTT CGATTCCACC   601 GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC   661 CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT   721 TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA   781 CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG   841 TCGANCTCTA NCTAGAGCTT GNCGTAATCN TGGNCATAGC TGTTTCCTGT GTGAAATTGT   901 TATCCGCTCA CNATTCCACA CNACATACNA NCNNGAAGCA TAAAGNGTAA ANNNNGGGGG   961 NGNCTNNNGA NTGANCTNNC TNNNATTNNN TNGNNNNNNN NNCACNGNCN NNNTTNCNNN 1021 NNGNNANNNN NNNNNGNNNN NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl