Please review the QC test results indicated by icon below as well as clone information before placing your order.

CSII FLAG-hRFWD3 C315A/I639K

Lentivirus expression vector of human ring finger and WD repeat domain 3 (RFWD3) mutant, C315A/I639K (C315A, RING finger domain mutation. I639K, chromatin localization-defective mutation).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Inano, S et al. Mol. Cell, 66(5): 622-634, 2017)
Additional terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Ordering	Please visit Ordering instruction.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Remarks	Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Inano, S et al. Mol. Cell, 66(5): 622-634, 2017)。
付加的提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。 提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
備考	Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17500_A9Iop1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: hRFWD3-N_R Region: insert 5', attB1, FLAG, CMV pro 2, CMV en 2 Sequence file: RDB17500_A9Ioa.seq
>D06551B2_A9Io_1_hRFWD3-N_R_C02_08_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNTNNNN TNNGCANGNN CTGGCTGGAN GNNGGATGGT    61 ACCCCCTGGC TGCTGACCAC ATCAGCAGGA ACAGGCTGGA GGAGGGCTGG TCCCCCTTGG   121 CTGCTGGCCA TGCCAGCAGG AGCTGGCTGT TGTTCGGCAT GATTTAACTG CACCTGAACA   181 TCATATTCCA TTGCTTCATG AGCCATGGTG AAGGGGGCGG CCGCGGAGCC TGCTTTTTTG   241 TACAAACTTG TTTGATAGCT TGGACCAGAA CCACCATGCT TATCGTCATC ATCTTTATAA   301 TCCATGGTGC TAGCCAGCTT GGGTCTCCCT AGCGGATCTG ACGGTTCACT AAACCAGCTC   361 TGCTTATATA GACCTCCCAC CGTACACGCC TACCGCCCAT TTGCGTCAAT GGGGCGGAGT   421 TGTTACGACA TTTTGGAAAG TCCCGTTGAT TTTGGTGCCA AAACAAACTC CCATTGACGT   481 CAATGGGGTG GAGACTTGGA AATCCCCGTG AGTCAAACCG CTATCCACGC CCATTGATGT   541 ACTGCCAAAA CCGCATCACC ATGGTAATAG CGATGACTAA TACGTAGATG TACTGCCAAG   601 TAGGAAAGTC CCATAAGGTC ATGTACTGGG CATAATGCCA GGCGGGCCAT TTACCGTCAT   661 TGACGTCAAT AGGGGGCGTA CTTGGCATAT GATACACTTG ATGTACTGCC AAGTGGGCAG   721 TTTACCGTAA ATACTCCACC CATTGACGTC AATGGAAAGT CCCTATTGGC GTTACTATGG   781 GAACATACGT CATTATTGAC GTCNATGGGC GGGGGTCGTT GNGCGGTCAG CCAGGCGGGC   841 CATTTACCGT AAGTTATGTA ACGCGGANNT CCNTATATGG NCNATGAACT AATGNNCCCG   901 TAATTGATTA CTATTAATCG AANTAANTCN AAGTGAANTT CNNNNNNCCN NNTANTAAAC   961 CCNAAAATTT TGAATTTTNG NANNTTGNTT TTGNANTTNN TNANNTNNNN NNNNNGNTNN 1021 NNNNNANNNN NNNNNN //
Primer: IRES_R3 Region: attB2, insert 3' (I639K) Sequence file: RDB17500_A9Iob.seq
>D06551B2_A9Io_1_IRES_R3_C06_09_ABI24.ab1     1 NNNNNNNNNN NNGNNNNNNG NNGGNNNNGG GNGGNNCCGC GGCCGCTGCA GAATTCTCGA    61 TGGCAACTAG AAGGCACAGT CGAGGCTGAT CAGCGGGTTT ATCGAACCAC TTTGTACAAG   121 AAAGCTGGGT CGGCGCGCCC ACCCTTTCAC TCCCACTTAT AGATGTGGAC CATCTTCTCT   181 GTTAAGGTAG CCAAGTAGCT GTTACGGTTC ACCTCAAATG GGCAGATGTC CAACACAGGC   241 TGATCGGTCT GTAGGTCCTG GAGCAACGAG CCACTGGCAG CATCCCACAG CAGGGCAGAA   301 TTTGCTGCTT CATCCCCAGT ACACACCAGG ATGTTGCCAT CATTCTCTGG GCTTTGGAAA   361 ATGGCATTTT TGGTCAATAG TTTGCAAGTA GGTCCTCCAA AAAATGTATG TACAGGCTGG   421 CAGGAGCAGA TTGGATTTCC AGTGTCATCC AGTCGGTAGG ACATTTCCAT CAGCACACTT   481 CGTATGGTGG TGTGATTTTT ATCAGGCCTG TAGGTCACAA GACAGTGCCG GGAGCTGTTC   541 TCTGTCTGAA AGTCTTTGCA CCCCCCTGGC TCCAAGGGCA GCACATGAGG CCAATGAGAA   601 AAGTCCATTT TCTGTTCCCA GAATGAAGCA TCCTCCAAGG TTCCAGCCAG CACCCCACCA   661 TATGGAAATG CAGCTGAGGC AGCTCTGGGC ATGTATGACA GGGAGACCAG TGGGCATCTG   721 GCTTTCTGAG CTACTAACTC CTGCACATGA CTGCTCGTGT TTCGCACGTC ATATACCAGA   781 ATTGAACCAT TGGCCAGTCC AGCATAGATG TAGTTAGCCT CATCAAGACA CCAGCAACAG   841 CTCCAGACAG GACGTCCAGC ATTATAAGTC TGGACCACGG NATNNGTCTC CAGGCTGGNC   901 AGTTNAATAG NGTTGTCNAN GGAANCAGAN ANTANCAAGC NNCTNANGNN NNNGCNNAAC 961 GCCAGNNCNC GNNNCTNNTT GNNNTGCATN NNNNNNNNN //
Primer: hRFWD3-N_F Region: insert mid (C315A) Sequence file: RDB17500_A9Ioc.seq
>D06551B2_A9Io_1_hRFWD3-N_F_E01_05_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNTCTGANNN TTGCAGGTGT CTAGTAGTTC TGATTCTGAC    61 AGTGACAGCT CTGCAGAGTA TGGAGGGGTT GTTGACCAGG CAGAGGAATC TGGAGCTGTC   121 ATTTTAGAAG AGCAACTAGC AGGTGTCTCA GCAGAGCAAG AAGTTACATG TATCGATGGA   181 GGCAAGACCC TCCCCAAACA GCCATCTCCC CAGAAGTCTG AGCCTCTGCT ACCTTCTGCT   241 TCTATGGATG AGGAAGAAGG GGACACTTGT ACAATATGTC TGGAACAGTG GACCAATGCT   301 GGGGACCACC GGCTCTCAGC ATTACGCTGT GGGCATCTCT TTGGGTATAG GGCCATTTCC   361 ACGTGGCTTA AAGGACAAGT ACGAAAATGT CCCCAGTGCA ACAAGAAAGC CAGGCACAGT   421 GACATTGTCG TCCTTTATGC CCGAACCCTG AGAGCTTTGG ACACTAGTGA ACAGGAGCGC   481 ATGAAAAGTT CCCTACTGAA GGAACAGATG CTAAGGAAAC AGGCCGAGTT AGAATCAGCA   541 CAGTGCCGAC TCCAACTGCA GGTCCTCACT GATAAGTGCA CTAAGCTTCA AAGGCGTGTT   601 CAGGACTTGC AAAAACTTAC GTCACATCAA AGTCAGAATT TACAGCAACC CAGGGGCTCC   661 CAAGCATGGG TCCTGAGCTG CTCACCCTCC AGCCAGGGCC AGCACAAGCA CAAGTACCAC   721 TTCCAAAAGA CCTTCACAGT ATCTCAGGCA GGAAACTGCC GGATCATGGC ATACTGTGAT   781 GCTCTGANCT GCCTGGTGAT ATCACAGCCT TCTCNNCAGG CCTCTTTTCT TCCANGCTTT   841 NGTGTTAAGA TGTTGAGTAC NGNCNNCATG AAGAGCAGTC AGTACATTCC GATGCATGGC   901 AAACANATCC GNNNNNTGNC GTTTANCNNT TANNTCNNAN GNTTGCTACT CTCTGNTNNC 961 CNANNNNNNN NNATTAAACN NANNNNCNNN NNNNNNN //
Primer: HIVgp_R1 Region: HIV-1 psi, 5' LTR Sequence file: RDB17500_A9Iod.seq
>D06551B2_A9Io_1_HIVgp_R1_C03_09_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN CTCTTTTTTC TCTCTGCNCC NCTCTTCTCT TTGCCTTGGT    61 GGGTGCTACT CCTAATGGTT CAATTTTTAC TACTTTATAT TTATATAATT CACTTCTCCA   121 ATTGTCCCTC ATATCTCCTC CTCCAGGTCT GAAGATCAGC GGCCGGCCGC TTGCTGTGCG   181 GTGGTCTTAC TTTTGTTTTG CTCTTCCTCT ATCTTGTCTA AAGCTTCCTT GGTGTCTTTT   241 ATCTCTATCC TTTGATGCAC ACAATAGAGG GTTGCTACTG TATTATATAA TGATCTAAGT   301 TCTTCTGATC CTGTCTGAAG GGATGGTTGT AGCTGTCCCA GTATTTGTCT ACAGCCTTCT   361 GATGTTTCTA ACAGGCCAGG ATTAACTGCG AATCGTTCTA GCTCCCTGCT TGCCCATACT   421 ATATGTTTTA ATTTATATTT TTTCTTTCCC CCTGGCCTTA ACCGAATTTT TTCCCATCGC   481 GATCTAATTC TCCCCCGCTT AATACTGACG CTCTCGCACC CATCTCTCTC CTTCTAGCCT   541 CCGCTAGTCA AAATTTTTGG CGTACTCACC AGTCGCCGCC CCTCGCCTCT TGCCGTGCGC   601 GCTTCAGCAA GCCGAGTCCT GCGTCGAGAG AGCTCCTCTG GTTTCCCTTT CGCTTTCAAG   661 TCCCTGTTCG GGCGCCACTG CTAGAGATTT TCCACACTGA CTAAAAGGGT CTGAGGGATC   721 TCTAGTTACC AGAGTCACAC AACAGACGGG CACACACTAC TTGAAGCACT CAAGGCAAGC   781 TTTATTGAGG CTTAAGCAGT GGGTTCCCTA NTTAGCCAGA NAGCTCCCAG GCTCANATCT   841 GGTCTAACCA GAGAGANNNA GTACAGGCAA AACGCGCTGC TTATATAGAC CTCCCACCGN   901 NCACGCCTAC CGNCCATTTG CGTCNANGGG GGCGGANTTG TNACGANNTT TTGGAAAGTC   961 CCGTTGNTTT TGGTGCCAAA NNAACNNCCN TNGACNTCNN NGGGGNNNNN NNNTTNGNNN 1021 NNCCNNNNNN NCNNNNCNCN NNNNNNNNCN NNNNNNNNN //
Primer: cPPT+CTS_v2_F Region: cPPT_CTS, CMV en 2, CMV pro2 Sequence file: RDB17500_A9Ioe.seq
>D06551B2_A9Io_1_cPPT+CTS_v2_F_C01_03_ABI08.ab1     1 NNNNNNNNNC NNNNNNNNNN NNNTNNNNNN NNNNATTACN AAAATTCAAA ATTTTCGGGT    61 TTATTACAGG GACAGCAGAA ATTCACTTTG AATTAATTCG ATTAATAGTA ATCAATTACG   121 GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC   181 CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC   241 ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT   301 GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT   361 GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT   421 TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC   481 ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC   541 GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC   601 TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACNGT GGGAGGTCTA TATAAGCAGA   661 GCTGGTTTAG TGAACCGNNN NATCNGCTAG GGAGANCCAN NCTGGCTAGC ACCNTGGANT   721 ATAAAGATGA TGACGATAAG CATGGTGGTT CTGGTCCAAG CTATCNNACA AGTTTGTACA   781 AAAAAGCANG CTCCGCNGNC GCCCCCTTCA CCNTGGCTCA TGAAGCAATG GAATATGANG   841 TTCANNTGCN GTTAAATCAT GCCNNANNAA CAGCCAGCTC NNGNNNNNNN TGGCCNNNNN   901 NNNANGGGGA NNNANCCNNN CNTNCNNNNT GTNNCTGCTG ATGNNNNNAG CNNNCNAGGG 961 GNNNNNNNNN N //
Primer: IRES-F Region: IRES2, BSD, WPRE Sequence file: RDB17500_A9Iof.seq
>D06551B2_A9Io_1_IRES-F_D01_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNTCGNNGN NNNNNNNNNT CTAGGCCCCC CGAACCNCGG    61 GGNNGTGGTT TTCCTTTGAA AAACACGATG ATAATATGGC CACAACCATG GCCAAGCCTT   121 TGTCTCAAGA AGAATCCACC CTCATTGAAA GAGCAACGGC TACAATCAAC AGCATCCCCA   181 TCTCTGAAGA CTACAGCGTC GCCAGCGCAG CTCTCTCTAG CGACGGCCGC ATCTTCACTG   241 GTGTCAATGT ATATCATTTT ACTGGGGGAC CTTGTGCAGA ACTCGTGGTG CTGGGCACTG   301 CTGCTGCTGC GGCAGCTGGC AACCTGACTT GTATCGTCGC GATCGGAAAT GAGAACAGGG   361 GCATCTTGAG CCCCTGCGGA CGGTGCCGAC AGGTGCTTCT CGATCTGCAT CCTGGGATCA   421 AAGCCATAGT GAAGGACAGT GATGGACAGC CGACGGCAGT TGGGATTCGT GAATTGCTGC   481 CCTCTGGTTA TGTGTGGGAG GGCTAAGTCG AGCCTCTAGA GTTAACATCG AGGGATCAAG   541 CTTATCGATA ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCTTAAC   601 TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT   661 GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGNTTGCT GTCTCTTTAT   721 GANGAGTTGT GGNCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT TGCTGACGCA   781 ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCNGGAN TTTCGCTTTC   841 CCCNNCCCTA TTGNCACGGC GGAANTCATC GCCGNCNGNC TTGCCCGCTG CTGGANANGG   901 GCTCNNNTGT NNGGNACTGA NNATTCNNNN GNNGTTGNCN GGGAANCTGA CNNNCNTTNN 961 NNNNGCNGNT CNNCNNGNNN TNGNNNNNNN NNN //
Primer: f1ori_R3 Region: bGH p(A), 3' LTR Sequence file: RDB17500_A9Iog.seq
>D06551B2_A9Io_1_f1ori_R3_E03_15_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNGGNN TCTTTCNGCC TCNGAANCCN TAGAGCCCAC    61 CGCATCCCCA GCATGCCTGC TNNNNNNTTC CCAATCCTCC CCCTTGCTGT CCTGCCCCAC   121 CCCACCCCCC AGAATAGAAT GACACCTACT CAGACAATGC GATGCAATTT CCTCATTTTA   181 TTAGGAAAGG ACAGTGGGAG TGGCACCTTC CAGGGTCAAG GAAGGCACGG GGGAGGGGCA   241 AACAACAGAT GGCTGGCAAC TAGAAGGCAC AGTCGAGGCT GATCAGCGGG TTTAAACGGG   301 CCCTGCTAGA GATTTTCCAC ACTGACTAAA AGGGTCTGAG GGATCTCTAG TTACCAGAGT   361 CACACAACAG ACGGGCACAC ACTACTTGAA GCACTCAAGG CAAGCTTTAT TGAGGCTTAA   421 GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT CCCAGGCTCA GATCTGGTCT AACCAGAGAG   481 ACCCAGTACA GTCCGGATGC AGCTCTCGGG CCATGTGATG AAATGCTAGG CGGCTGTCAA   541 ACCTCCACTC TAATACTTCT CTCTCCGGGT CATCCATCCC ATGCAGGCTC ACAGGGTGTA   601 ACAAGCGGGT GTTCTCTCCT TCATTGGCTT CTTCTACCTT CTCTTGCTCA ACTGGTACTA   661 GCTTGTAGCA CCATCCAAAG GTCAGTGGAT ATCTGATCCC TGGCCCTGGT GTGTAGTTCT   721 GCCAATCAGG GAAGTANNCT TGTGTGTGGT AGATCCACAG ATCAAGGATA TCTTGTCTTC   781 GTTGGGAGTG AANTNNNNCT TCCNGTCCCC CCTTTTCTTT TAAAAAGTGG CTAAGATCTA   841 CAGCTGCCTT GTAAGTCATT GGTCTNNAAN GGTACCTGAN GTGTGANTGG NNAAAANCCC   901 ACCTCCTCCT CCTCNTNGTG CTTNNTAGCC NGGCACNATC AGNNNTTGGN ANCTGCTGNA   961 NNGNNNCTTG TGATTGCTCC NNGNTTNTTC NNNGNNNNNA CNGNANNGAT GNNNGGNNNN 1021 NNGNCNNNNN GNNNANNCGN NNCNNNTGNA GGNCNNANNN NNNNNNN //
Primer: SV40pro_ori_F Region: SV40 pro_ori, EM7 pro, BleoR, SV40 p(A) Sequence file: RDB17500_A9Ioh.seq
>D06551B2_A9Io_1_SV40pro_ori_F_G03_21_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNCTC TGCCTCTGNN CTATTCNNGA AGTAGTGAGG    61 AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT   121 CGGATCTGAT CAGCACGTGT TGACAATTAA TCATCGGCAT AGTATATCGG CATAGTATAA   181 TACGACAAGG TGAGGAACTA AACCATGGCC AAGTTGACCA GTGCCGTTCC GGTGCTCACC   241 GCGCGCGACG TCGCCGGAGC GGTCGAGTTC TGGACCGACC GGCTCGGGTT CTCCCGGGAC   301 TTCGTGGAGG ACGACTTCGC CGGTGTGGTC CGGGACGACG TGACCCTGTT CATCAGCGCG   361 GTCCAGGACC AGGTGGTGCC GGACAACACC CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC   421 GAGCTGTACG CCGAGTGGTC GGAGGTCGTG TCCACGAACT TCCGGGACGC CTCCGGGCCG   481 GCCATGACCG AGATCGGCGA GCAGCCGTGG GGGCGGGAGT TCGCCCTGCG CGACCCGGCC   541 GGCAACTGCG TGCACTTCGT GGCCGAGGAG CAGGACTGAC ACGTGCTACG AGATTTCGAT   601 TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG   661 ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTTGTTTATT   721 GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACNA ATTTCACAAA TAAAGCATTT   781 TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGT   841 ATACCGTCGA NNTCTANCTA GAGCTTGGNC GTANTCATGG TCNTAGCTGT TNCCTGTGTG   901 AAATTGNTNN TCCGCTCACA ATTNCNCACA ANATACGANC CNNGNANNCN TAAAGNGTAA   961 AGNCNGGGGG TGNCTAANNN NTGAGCTNNN TCNNNNTNNN NNGCGTTGCG CNNNNNGNCC 1021 NGCTTTNNCN NNNNGGNAAA NCNNNNCGNN NNNNNNCNNC NTTNANNNNN NNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl