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pCOLA-3C-eIF2Be-eIF2Bg

Bacterial expression vector of human eukaryotic translation initiation factor 2B subunit gamma (eIF2Bgamma) and human eukaryotic translation initiation factor 2B subunit epsilon (eIF2Bepsilon).

Catalog number RDB17724
Resource name pCOLA-3C-eIF2Be-eIF2Bg
Alternative name eIF2Bgamma-epsilon
Clone info. Bacterial expression vector of human eukaryotic translation initiation factor 2B subunit gamma (eIF2Bgamma) and human eukaryotic translation initiation factor 2B subunit epsilon (eIF2Bepsilon) cloned into the pCOLADuet-1. An HRV 3C protease cleavable His6-tag was added to the N-terminus of eIF2Bepsilon.
Comment Expression was confirmed by the depositor.
Vector backbone pCOLADuet-1 (plasmid)
Size of vector backbone 3.7 kb
Selectable markers Kanamycin
Gene/insert name human EIF2B3 cDNA human EIF2B5 cDNA
Depositor|Developer Ito, Takuhiro |
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External Database
human EIF2B3 human EIF2B5

        Reference sequence
          

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kashiwagi, K. et al. Science, 364(6439), 495-499, 2019).
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        提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kashiwagi, K. et al. Science, 364(6439), 495-499, 2019)。
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        RDB17724 pCOLA-3C-eIF2Be-eIF2Bg DNA solution

        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Electronic file

        Original reference

        original Kashiwagi, K., Structural basis for eIF2B inhibition in integrated stress response. Science 364 (6439): 495-499 (2019). PMID 31048492.

        Further references such as user reports and related articles (go to bottom)

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        Sequence information

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB17724_B0Adp1-1.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: lacI-N_R2
        Region: lacI pro, T7 pro-1, lac OP, RBS, 6xHis, insert-1 5'
        Sequence file: RDB17724_B0Ada.seq check
        >D06721A3_B0Ad_3_lacI-N_R2_A01_01_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG GCATACTCTG CGACNTCNNA
           61 TAANCGTTAC TGGTTTCNCA TTCNCCACCC TGAATTGACT CTCTTCCGGG CGCTATCATG
          121 CCATACCGCG AAAGGTTTTG CGCCATTCGA TGGTGTCCGG GATCTCGACG CTCTCCCTTA
          181 TGCGACTCCT GCATTAGGAA ATTAATACGA CTCACTATAG GGGAATTGTG AGCGGATAAC
          241 AATTCCCCTG TAGAAATAAT TTTGTTTAAC TTTAATAAGG AGATATACCA TGGGCAGCAG
          301 CCATCACCAT CATCACCACA GCCAGGATCC GAATTCGAGC TCGGCGCGCC TGCAGGTCGA
          361 CGCGGCCCCT GTAGTGGCGC CGCCTGGTGT GGTGGTTAGT CGGGCTAACA AGCGCAGCGG
          421 CGCGGGGCCG GGAGGCAGCG GTGGCGGGGG AGCCAGAGGG GCGGAGGAGG AACCGCCGCC
          481 GCCCCTACAA GCAGTTCTGG TGGCCGATAG CTTCGATCGC CGCTTCTTCC CCATCTCCAA
          541 GGACCAGCCT CGGGTCCTCT TGCCCCTGNN NNNNGTGGCA TTAATTGACT ACACTCTGGA
          601 ATTCCTGACT GCCACAGGTG NNNNNGAAAC ATTTGTCTTT TGTTGCTGGA AAGCTGCTCA
          661 AATCAAAGAA CATTTACTGA AGTCAAAGTG GTGCCGCCCT ACNTCTCTCN NTGTGGTTCG
          721 AATAATTACA TCANANCTCT ATCNNNTCAC TGGGAGATGT CCTCCGNGAT GTTGATGCCN
          781 ANGGCTTTTG GNGCGCTCTG ANTTTCTTCT GGNGTATGGG GATGTCATCT CNANNNNNNN
          841 AATATCACCA NANCCCTTNG ANGAANNNAN GTTNNANANG GAANNNTNNN AAAAAANNGT
          901 TTTCTGTGAT GACNATGANC NTTNNAGGAN TNNNNNNNCN ANNCNNNCCN NNTNNNNTNN
        961 NNNCNNANNA NAANNGNNGN TANNNNNNNN NN
        //
        Primer: hEIF2B3_R
        Region: insert-2 5', lac OP, T7pro-2, insert-1 3'
        Sequence file: RDB17724_B0Adb.seq check
        >D06721A3_B0Ad_3_hEIF2B3_R_A02_01_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN TNNNNNNNGG NANCAGANGT TTGGGAATGC
           61 TGGAAGTTAG GTCTGTCATC CGAGNNCNNC CACCTACTGC CATCACTACT GCTTGAAATT
          121 CCATATGTAT ATCTCCTTCT TATACTTAAC TAATATACTA AGATGGGGAA TTGTTATCCG
          181 CTCACAATTC CCCTATAGTG AGTCGTATTA ATTTCGATTA TGCGGCCGTG TACAATACGA
          241 TTACTTTCTG TTCGACTTAA GCATTATGCG GCCGCTCAGT CATCTTCAGA TGACTCCTCT
          301 TCTGCCTCTT TTAGCCACTG GATGAACCTC TGCAGCTGTT GATTCTTGCG CAACTGCTGG
          361 CCCTTGTCAG TTGTATCTCT TTGGCTGAAC CAGCTCAGAA TTGTTTCCTC AGCCAGGATC
          421 TCCAGCTGGT AGAAAGCCAT CAGTACCTTG GCCATGGAAA TACCAAGAGC TTCATGCTCT
          481 AGGAAGAAGT CCTCAATGGC TGCTAACGCT TCCAAATGGT CGGCTGCGCG CTTTATGTAG
          541 TTCCTAAAAA CAGGGCTCCA GGCCTTTAGC AGAGGAAGCA GCAGGGCACA GTAGCGGCTT
          601 GAGTCAAGCG GGGAATCCAT CTGTTGCAGG GGGAACTCCA GGACCACGTG GCTCAGTACC
          661 TGCATCACCT CCTTTAGACT TATGTTATAG GCATACTTGA GAGAGTTGAT TTCCAGGACG
          721 AGATTGTCAC AAGAAATGTT CTCCTCTTTG CCCCGCTGTA NTGTTCCTAA AACTTCATTC
          781 TGGAACACTT TGATGTCATC CATCTGANGG GAGCCTCCCC GGCTGTCCGG CTCCTCANAA
          841 TCCATACTTT GCTCACTTTC ACTTTCACTC TCTNCTTCCN TGTTGATCTT GAGTCCCCNC
          901 AGANTCTGCT GCANTTCNNN CTCNTCCNCC ANGTTCATGC CTGCANCTTT CNNNANGTAG
          961 NCNTTGNCAN CAGCTCCTAC TTNCTGCTGN ANNGNANNNN NTNNNNTNCN CNTTNGNCCN
        1021 TTTNCTNNGN NNNNNCNNNN NNNNNTNNNN NNANNTCNNN NNNNNNN
        //
        Primer: T7termi3'_R
        Region: T7 ter, insert-2 3'
        Sequence file: RDB17724_B0Adc.seq check
        >D06721A3_B0Ad_1_T7Termi3'_R_D04_10_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN CTGNNNNNNC GGNTNNTTGA CNACCGGAAG
           61 CAGTGTGNNC GTGTGCTTCT CNNATGCCTG AGGTTTCAGC AAAAAACCCC TCAAGACCCG
          121 TTTAGAGGCC CCAAGGGGTT ATGCTAGTTA TTGCTCAGCG GTGGCAGCAG CCTAGGTTAA
          181 TTAAGCTGCG CTAGTAGACG AGTCCATGTG CTGGCGTTCA AATTTCGCAG CAGCGGTTTC
          241 TTTACCAGAC TCGAGGGTAC CGACGTCTCA GATCTCCATG AGCTGGTCAT TCCCCACGAT
          301 CACCTCATTC ACTCGTTTAG CTTTGGCTTC AATCCTCTGG CCACTTCCAA TCAAGCAGTC
          361 CTTGATGTCT GCACCCTTCT CGATCACAGC ATTGTTGCAG ATGACACTGC CTTGGATATT
          421 GCTTCCTTCC TCCACAGTGA CTGAGTTCAT GAGAAGGCAA TTGGTAATAG TCACTCTATC
          481 TTTTATGAGA CAGGATGAGC CAATGACTGA GCGCTTAATG GATGACTTCT CTCCAATCTG
          541 TGTCTCTGGC CCAATGAGGC TGTCAACTCC AACCAGGTGT TTGCTGACAA TCTGGGCTGA
          601 CGAATGGACT GGTGGTTCTT CTGGACAGAG AGCAGACAGC AATTTGGGCA CCTGTCTGTT
          661 TGCTTCCATG TAGAGTCCCA NNGNGCTCAC TCGAGAGCAG AGCCCCTCTT TCATGATGTG
          721 GACATAGCAG CGCACCTGTG ATCTGGACAA GTCTTCCCAC CTGTCTCCTC GACAGGCATT
          781 CCAGCAGGCA TCATANGGGA GCCAGGNTCA GTGTATTGGN CTTCTTTTTA TAAANNTGTA
          841 GATATCNNNN GNNTTCANCN NCNTTTTNNT TTAGATCCNC CTCTTTTTCT NNTNGNNCCC
          901 TGTTGNGAGG AANCTGANGA AANNTGTTTT CTCACTAAAT ATGGNNNNNN NNCACTNCNN
          961 GATAGANNNN TANTNNANCN CNTTTTNNNN NTNNGNNAAN CCNNNGATNN NTTTTTTTCN
        1021 AANNNNNNNN NN
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Kashiwagi, K., Structural basis for eIF2B inhibition in integrated stress response. Science 364 (6439): 495-499 (2019). PMID 31048492.

        2022.05.17

        GNP_filter3_RDBDEP_html_220516.pl