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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEBMulti-Neo-human-eIF2alpha

Expression vector of human eukaryotic translation initiation factor 2 subunit alpha (eIF2alpha).

Catalog number RDB17725
Resource name pEBMulti-Neo-human-eIF2alpha
Alternative name eIF2alpha
Clone info. Expression vector of human eukaryotic translation initiation factor 2 subunit alpha (eIF2alpha) cloned into the pEBMulti-Neo.
Comment Expression was confirmed by the depositor.
Vector backbone pEBMulti-Neo (plasmid)
Size of vector backbone 10.0 kb
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cells).
Gene/insert name human EIF2S1 cDNA
Depositor|Developer Ito, Takuhiro |
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External Database
human EIF2S1

          Reference sequence
            
           
          Sequence (partial) RDB17725pka01.seq provided by the depositor

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kashiwagi, K. et al. Science, 364(6439), 495-499, 2019).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kashiwagi, K. et al. Science, 364(6439), 495-499, 2019)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB17725 pEBMulti-Neo-human-eIF2alpha DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Kashiwagi, K., Structural basis for eIF2B inhibition in integrated stress response. Science 364 (6439): 495-499 (2019). PMID 31048492.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB17725_A9Kcp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pCAG-F_V3
          Region: CAG pro, insert 5'
          Sequence file: RDB17725_A9Kca.seq check
          >D06721A4_A9Kc_1_pCAG-F_V3_E02_14_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNTN NNNNNNNNNN GGNNNGNCGG GGTTCGGCTT CTGGCGTGTG
             61 ACCGGCGGCT CTANNNCCTC TGCTAACCAT GTTCATGCCT TCTTCTTTTT CCTACAGCTC
            121 CTGGGCAACG TGCTGGTTAT TGTGCTGTCT CATCATTTTG GCAAAGAATT AACCCTCACT
            181 AAAGGGGTAC CGGGCCCCCC CTCGAGGTCG ACGCCACCAT GCCGGGTCTA AGTTGTAGAT
            241 TTTATCAACA CAAATTTCCT GAGGTGGAAG ATGTAGTGAT GGTGAATGTC AGATCCATTG
            301 CTGAAATGGG GGCTTATGTC AGCTTGCTGG AATACAACAA CATTGAAGGC ATGATTCTTC
            361 TTAGTGAATT ATCCAGAAGG CGTATCCGTT CTATCAACAA ACTCATCCGA ATTGGCAGGA
            421 ATGAGTGTGT GGTTGTCATT AGGGTGGACA AAGAAAAAGG ATATATTGAT TTGTCAAAAA
            481 GAAGAGTTTC TCCAGAGGAA GCAATCAAAT GTGAAGACAA ATTCACAAAA TCCAAAACTG
            541 TTTATAGCAT TCTTCGTCAT GTTGCTGAGG TGTTAGAATA CACCAAGGAT GAGCAGCTGG
            601 AAAGCCTATT CCAGAGGACT GCCTGGGTCT TTGATGACAA GTACAAGAGA CCTGGATATG
            661 GTGCCTATGA TGCATTTAAG CATGCAGTCT CAGACCNATC TATTTTGGAT AGTTTAGATT
            721 TGAATGAAGA TGAACGGGAA GTACTCATTA ATAATATTAA TAGGCGCTTG ACCCCACAGG
            781 CTGTCAAAAT TCGAGCAGAT ATTGAAGTGG CTTGTTATGG TTATGAANGC ATTGATGCTG
            841 TAAAAGAAGC CCTAAGAGCN NGTTTGAATT GTTCTACAGA AAACATGCCN ATTAAGANTA
          901 ATCTAATAGC TCNNNCNNNG NATGTNNTGN CTACNNNNNN NNNN
          //
          Primer: SV40polyA_R2
          Region: SV40 pA, insert 3'
          Sequence file: RDB17725_A9Kcb.seq check
          >D06721A4_A9Kc_1_SV40polyA_R2_H05_08_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNTGCATTC NTTTTATGTT
             61 TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG TAAAACCTCT ACAAATGTGG
            121 TATGGCTGAT TATGATCTAG AGTCGCGGCC GCTTAATCTT CAGCTTTGGC TTCCATTTCT
            181 TCTGCATCAT CATCTCCATC CACTTCGGCA TTTTCTCTTT CAAGCCTCTC CATCTGCCTC
            241 GCAAGTTCAG TCTCATCTGT ATCTGTGACC ACTTTGGGCT CCATTTGAAC ATTGAACACA
            301 CCCCTCTTTT CCTCAATCTT CTCTTTGATA ACAGCCATAG CTTGACTGAG GACAGAAAGG
            361 CCTTCTGTTC TCTCCAGGGT TGTCGTAGTC ATTACATACC GAGGAGGAGC TATTAGATTA
            421 ATCTTAATGG GCATGTTTTC TGTAGAACAA TTCAAACCTG CTCTTAGGGC TTCTTTTACA
            481 GCATCAATGC CTTCATAACC ATAACAAGCC ACTTCAATAT CTGCTCGAAT TTTGACAGCC
            541 TGTGGGGTCA AGCGCCTATT AATATTATTA ATGAGTACTT CCCGTTCATC TTCATTCAAA
            601 TCTAAACTAT CCAAAATAGA TGGGTCTGAG ACTGCATGCT TAAATGCATC ATAGGCACCA
            661 TATCCAGGTC TCTTGTACTT GTCATCAAAG ACCCAGGCAG TCCTCTGGAA TAGGCTTTCC
            721 AGCTGCTCAT CCTTGGTGTA TTCTAACACC TCAGCAACAT GACGAAGAAT GCTATAAACA
            781 GTTTTGGANT TTGTGAATTT GTCTTCACAT TTGATTGCTT CCTCTGGANA AACTCTTCTT
            841 TTTGACAAAT CNATATATCC TTTTTCNTTG TCCACCNTAA TGACAACCAC ACACTCATTC
            901 CTGCCAATTC NGATGANTTT GTTGATANAN NGGANACNCC NTNNTGGANA NTTCNCTNNN
            961 ANNAANCNTG CCTNNNNTGN TGNTGTATTC CANCAANCTG ANNTAAGCCC CNNNTTTCNN
          1021 NNNNGNANNT GANANTNNNN NNNNNN
          //
          Primer: SV40pro_ori_F
          Region: SV40 pro_ori, NeoR_KanR
          Sequence file: RDB17725_A9Kcc.seq check
          >D06721A4_A9Kc_1_SV40pro_ori_F_G02_20_ABI24.ab1
              1 NNNNNNNNNN NNNGNCGCCT CNGCCTCTGN NCTANTCNNG AAGTAGTGAG GAGGCTTTTT
             61 TGGAGGCCTA GGCTTTTGCA AAGATCATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT
            121 TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA
            181 TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA
            241 GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAAGA
            301 CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA
            361 CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT
            421 CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG
            481 GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA
            541 GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA
            601 TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGAGCATGC CCGACGGCGA
            661 NGNTCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG
            721 CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GANCGCTATC ANGACATAGC
            781 GTTGGCTANC CGNGANATTG CTGAAGANCT NNGCGGCGAA TGGGCTGANN GCTNCCNCNN
            841 NCTTTACNGN ATCGCNGCTN CNNATTCNNA GCGNNTCGNC NNTCNATCNN CNTTNNNNNC
          901 NANNNNNNNN NN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kashiwagi, K., Structural basis for eIF2B inhibition in integrated stress response. Science 364 (6439): 495-499 (2019). PMID 31048492.

          2022.07.27

          GNP_filter3_RDBDEP_html_220727.pl