Please review the QC test results indicated by icon below as well as clone information before placing your order.

pEBMulti-Neo-human-eIF2beta

Expression vector of human eukaryotic translation initiation factor 2 subunit beta (eIF2beta).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kashiwagi, K. et al. Science, 364(6439), 495-499, 2019).
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kashiwagi, K. et al. Science, 364(6439), 495-499, 2019)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17726_A9Kcp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CAG_R1 Region: CAG pro, insert 5' Sequence file: RDB17726_A9Kca.seq
>D06721A5_B0Ju_1_CAG_R1_A09_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNCNNGCGG GCGCGGGGCG AANCGGTGCG GCGCCGGCAG    61 GAAGGAAATG GGCGGGGNNG GCCTTCGTGC GTCGCCGCGC CGCCGTCCCC TTCTCCCTCT   121 CCAGCCTCGG GGCTGTCCGC GGGGGGACGG CTGCCTTCGG GGGGGACGGG GCAGGGCGGG   181 GTTCGGCTTC TGGCGTGTGA CCGGCGGCTC TAGAGCCTCT GCTAACCATG TTCATGCCTT   241 CTTCTTTTTC CTACAGCTCC TGGGCAACGT GCTGGTTATT GTGCTGTCTC ATCATTTTGG   301 CAAAGAATTA ACCCTCACTA AAGGGGTACC GGGCCCCCCC TCGAGGTCGA CGCCACCATG   361 TCTGGGGACG AGATGATTTT TGATCCTACT ATGAGCAAGA AGAAAAAGAA GAAGAAGAAG   421 CCTTTTATGT TAGATGAGGA AGGGGATACC CAAACAGAGG AAACCCAGCC TTCAGAAACA   481 AAAGAAGTGG AGCCAGAGCC AACTGAGGAC AAGGATTTGG AAGCTGATGA AGAGGACACT   541 AGGAAAAAAG ATGCTTCTGA TGATCTAGAT GACTTGAACT TCTTTAATCA AAAGAAAAAG   601 AAGAAAAAAA CTAAAAAGAT ATTTGATATT GATGAAGCTG AAGAAGGTGT AAAGGATCTT   661 AAGATTGAAA GTGATGTTCA AGAACCAACT GAACCAGAGG ATGACCTTGA CATTATGCTT   721 GGCAATAAAA AGAAGAAAAA GAAGAATGTT AAGTTCCCAG ATGANGATGA AATACTAGAG   781 AAAGANGANG CTCTAGAAGA TGAAGANAAC AAAAAAGANG ATGGTATCTC NTTCAGTAAT   841 CAGACAGGCC CNGCTTGGGC AGGCTCNNAA AGAGACTACA CATACNANGA GCTGCNNAAT   901 CGAGNGNNCA NCATCATGAN GGAAAAGAAN NCNNNATNTN GNNNNCTGGG GANAAAANNA   961 AANTTGTCAN GAAANCNTNN NNNNNNCNNN NNNNANNNNN ANNNNAGAAA NNNTNNNNNN 1021 NNNN //
Primer: SV40polyA_R2 Region: SV40 pA, insert 3' Sequence file: RDB17726_A9Kcb.seq
>D06721A5_A9Kc_1_SV40polyA-R2_E04_13_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNANNNNNNN TAANAANNAC AATTGCATTC NTTTTATGTT    61 TCAGGTTCNG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG TAAAACCTCT ACAAATGTGG   121 TATGGCTGAT TATGATCTAG AGTCGCGGCC GCTTAGTTAG CTTTGGCACG GAGCTGTGCT   181 CGCTTGCCCG TGACAGCCTG GAAGCCGGTT TTGATACTGG CAACAGAACA TCTAGAATGA   241 CAAGTTTCGC ACTGTAGGAA ATAGAGTCGT GTGTCCTTCT GCAGGATTGT GTCCGGTGAT   301 CGGCATGTGT GACAAGTGAC ATATTCCTTG ATATATCTTC TCAAGACATT TTCTATCTGT   361 TTCTGTTGGA ATCTTCCTTT GATTACAAGT TGGTTATTAC CATCTATAGA ACCACTTGTA   421 CCCAATTCAG CCAACAAAAA TGCAAGGAGA TGTTTGGGCT GACGATGTAA TAGTTTACAG   481 ATATCTGTAA AGTTGACAAA AGAAGTTTTC TTGGTTCCTA CTCGGACGAC TTGTGGAGGT   541 TTCATGACAA ATTTCCTTTT CTCCCCAGCA ACCATATCTG GATTCTTTTC CCTCATGATG   601 TTGAACACTC GATTCAGCAG CTCCTCGTAT GTGTAGTCTC TTTCTGAGCC TGCCCAAGCA   661 GGGCCTGTCT GATTACTGAA TGAGATACCA TCATCTTTTT TGTTGTCTTC ATCTTCTAGA   721 GCTTCATCTT TCTCTAGTAT TTCATCCTCA TCTGGGAACT TAACATTCTT CTTTTTCTTC   781 TTTTTATTGC CAAGCATAAT GTCAAGGTCA TCCTCTGGTT CAGTTGGNTC TTGAACATCA   841 CTTTCAATCT TAAGATCCNT TACACCTTCT TCAGCNNCNT CAATATCAAA TATCTTTTTA   901 GTTTTTTTTC NTCTTTTNNT TTTGANTNAA NAANTTCANG TCNTCTAGAT CNNNNNNNNN 961 N //
Primer: oriP_F Region: oriP, EBNA-1 Sequence file: RDB17726_A9Kcc.seq
>D06721A5_A9Kc_1_oriP_F_G04_19_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGG GGCGTNNNTT TCGCTGCTTG TCCTTTTCCT    61 GCTGGTTGCT CCCATTCTTA GGTGAATTTA AGGAGGCCAG GCTAAAGCCG TCGCATGTCT   121 GATTGCTCAC CAGGTAAATG TCGCTAATGT TTTCCAACGC GAGAAGGTGT TGAGCGCGGA   181 GCTGAGTGAC GTGACAACAT GGGTATGCCC AATTGCCCCA TGTTGGGAGG ACGAAAATGG   241 TGACAAGACA GATGGCCAGA AATACACCAA CAGCACGCAT GATGTCTACT GGGGATTTAT   301 TCTTTAGTGC GGGGGAATAC ACGGCTTTTA ATACGATTGA GGGCGTCTCC TAACAAGTTA   361 CATCACTCCT GCCCTTCCTC ACCCTCATCT CCATCACCTC CTTCATCTCC GTCATCTCCG   421 TCATCACCCT CCGCGGCAGC CCCTTCCACC ATAGGTGGAA ACCAGGGAGG CAAATCTACT   481 CCATCGTCAA AGCTGCACAC AGTCACCCTG ATATTGCAGG TAGGAGCGGG CTTTGTCATA   541 ACAAGGTCCT TAATCGCATC CTTCAAAACC TCAGCAAATA TATGAGTTTG TAAAAAGACC   601 ATGAAATAAC AGACAATGGA CTCCCTTAGC GGGCCAGGTT GTGGGCCGGG TCCAGGGGCC   661 ATTCCAAAGG GGAGACGACT CAATGGTGTA AGACGACATT GTGGAATAGC AAGGGCAGTT   721 CCTCGCCTTA NGTTGTAAAG GGAGGTCTTA CTACCTCCAT ATACGAACAC ACCGGCGANC   781 CAAGTTCCTT CGTCGGTAGT CCTTTCTACG TGACTCCTAN CCNGGAGAGC TCTTANACCT   841 TCTGCAATGN TCTCAAATTT CNGGTTGNAA CCTCCNTGAN CACNATGCTT TCCNAACCAC   901 CCTCCTTTTT TGCGCCTGNC TNCNTCACCN NGACCCCGGG GNNNAGNGNT TGGGNCTTCT   961 CCTNNNCATC TGNGGNNNCT GNTCTATNGN TNCNNNGGGN NNGTNNNGNN NNNCNNCNGG 1021 GNNNNNNTNN NNNNNGTANN NNAAANNNNN NNNNTNNNNN NNNNN //
Primer: SV40pro_ori_F Region: SV40 pro_ori, NeoR_Kan_R Sequence file: RDB17726_A9Kcd.seq
>D06721A5_A9Kc_1_SV40pro_ori_F_F04_16_ABI24.ab1     1 NNNNNNNNNN NNNNCNNNNN NNNNNCTCGG CCTCTGAGCT ATTCCNGAAG TAGTGAGGAG    61 GCTTTTTTGG ANGCCTAGGC TTTTGCAAAG ATCATCAAGA GACAGGATGA GGATCGTTTC   121 GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT   181 TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT   241 CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC   301 TGCAAGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG   361 TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC   421 AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA   481 TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC   541 GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG   601 AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG AGCATGCCCG   661 ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA   721 ATGGNCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAN CGCTATCAGG   781 ACATAGCGTT GGCTACCCGT GATATTGNTG AANAGCTTGG CGGCGAATGG GCTGACCGCT   841 TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCNCCTTC TATCNNCTNC   901 TTGACGAGTN CTTCTGANCG GGANTCTNGN NNTCNAAATG ACCGANCAAG CGACNCCCNA   961 NCNGNCATCN CNAGATTTCN ATNCCNNNGN CNNNNTNNNA TNNNNNNNNG GCNTNCNGNN 1021 NNNNTTTNNN NNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.07.27

GNP_filter3_RDBDEP_html_220727.pl