Please review the QC test results indicated by icon below as well as clone information before placing your order.

pEBMulti-Neo-human-eIF2gamma-FlagHis8

Expression vector of human eukaryotic translation initiation factor 2 subunit gamma (eIF2gamma).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kashiwagi, K. et al. Science, 364(6439), 495-499, 2019).
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kashiwagi, K. et al. Science, 364(6439), 495-499, 2019)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17727_A9Kcp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CAG_R1 Region: CAC pro, insert 5' Sequence file: RDB17727_A9Kca.seq
>D06721A6_B0Ju_1_CAG_R1_B09_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNCNNNC GGGCGCGGGG CGNANCGGTG CGGCGCCGGC    61 AGGAAGGAAA TGGGCGGNNN NGNCCTTCGT GCGTCGCCGC GCCGCCGTCC CCTTCTCCCT   121 CTCCAGCCTC GGGGCTGTCC GCGGGGGGAC GGCTGCCTTC GGGGGGGACG GGGCAGGGCG   181 GGGTTCGGCT TCTGGCGTGT GACCGGCGGC TCTAGAGCCT CTGCTAACCA TGTTCATGCC   241 TTCTTCTTTT TCCTACAGCT CCTGGGCAAC GTGCTGGTTA TTGTGCTGTC TCATCATTTT   301 GGCAAAGAAT TAACCCTCAC TAAAGGGGTA CCGGGCCCCC CCTCGAGGTC GACGCCACCA   361 TGGCGGGCGG AGAAGCTGGA GTGACTCTAG GGCAGCCGCA TCTTTCGCGT CAGGATCTCA   421 CCACCTTGGA TGTTACCAAG TTGACGCCAC TTTCACATGA AGTTATCAGC AGACAAGCCA   481 CAATTAACAT AGGTACAATT GGTCATGTAG CTCATGGGAA ATCCACAGTC GTCAAAGCTA   541 TTTCTGGAGT TCATACTGTC AGGTTCAAAA ATGAACTAGA AAGAAATATT ACAATCAAGC   601 TTGGATATGC TAATGCTAAG ATTTATAAGC TTGATGACCC AAGTTGCCCT CGGCCAGAAT   661 GTTATAGATC TTGTGGGAGC AGTACACCTG ACGAGTTTCC TACGGACATT CCAGGGACCA   721 AAGGGAACTT CAAATTAGTC AGACATGTTT CCTTTGTTGA CTGTCCTGNC CACGATATTT   781 TGATGGCTAC TATGCTGAAC GGTGCAGCAG TGATGGATGC AGCTCTTCTG TTGATAGCTG   841 GTAATGAATC TTGCCCTCNN NCTCANANAT CGGAACACCT GGCTGCTATA GAGNTCATGA   901 AACTGAANCA TATTTTGATT CTACAAAATA AAAANTGATT TGGNNAAANA AANNTCNNNN 961 NANNNANCNN NNNNANCANA NNNNTNGCAN TTNNNCCNNN NNNNNNN //
Primer: SV40polyA_R2 Region: insert 3' (8xHis, FLAG, hEIF2S3) Sequence file: RDB17727_A9Kcb.seq
>D06721A6_A9Kc_1_SV40polyA-R2_B02_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NANNANGTTA ACNANNANNA TTGCATTCAT TTTATGTTTC    61 AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA AAACCTCTAC AAATGTGGTA   121 TGGCTGATTA TGATCTAGAG TCGCGGCCGC TCAATGATGA TGATGATGAT GATGATGTTT   181 ATCGTCATCG TCTTTGTAGT CGTCATCATC TACTGTTGGC TTGATTGTCA CTCCTCTTCT   241 TATCTGACCC CAACCAATTA AACGCCAGTG TTTTTCAACT CTTCGGCTAA GGGCAATTTT   301 TTCTCCTACC TCTGTGCACA CTGGATTGGT CAAAACAATT TTACCCAAAT CGGCCTTGAC   361 AGCACTAACT CTCCCTCCTG TTGACAGGGA TCCTATGTTC ACCATGAGCA CTTCATTCTT   421 AGACAGCTTT TGAACCTTTG CTGCTTTCTT GTCTCCTTCA GTGCGTACAC CTAGAAGCCG   481 TCTAAGCAGG AAATAGGAAA TTTCCAATTC TGTGAATATC TCAGGTAAAG CTCCGACTGC   541 ACCAAGTACT TGCCCCACCA TTCTGTCAGC CCGGCACAAA GTGGGGTCAA TTTTTGTTCC   601 AACTCCAATA ANANCGCCTG GAGCAGCATA TTGCANATCA TTATGCTCCG CAAAAAGTGA   661 TACAATTTTG GAAAAANATT GGTTTACNNC NTGAGTTTNC CTTCNNCTAT CTTTGNAAAC   721 AATACCAGGN CTTACNTTCT ATCTCCTGNN CNANCTTTNA ATACTCCNNT TTANGANNNT   781 NNCACNNNNT ACNNNTNCCC TNANNGNNNN NNANNTTCAC NNNNNNNGTT TGNTNNNCNT   841 CAAANATCTA NTAACAATAA GNCNGGGGCT CTGAANNAAA NNNTCNTGNG NGTNNNNGNN 901 NNTTNNNTTT NCNNNGNNNN NNNNCNANNN NNNNNNN //
Primer: oriP_F Region: oriP, EBNA-1 Sequence file: RDB17727_A9Kcc.seq
>D06721A6_A9Kc_1_oriP_F_D02_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG GGGCGTGNAT TTTCGCTGCT TGTCCTTTTC    61 CTGCTGGTTG CTCCCATTCT TAGGTGAATT TAAGGAGGCC AGGCTAAAGC CGTCGCATGT   121 CTGATTGCTC ACCAGGTAAA TGTCGCTAAT GTTTTCCAAC GCGAGAAGGT GTTGAGCGCG   181 GAGCTGAGTG ACGTGACAAC ATGGGTATGC CCAATTGCCC CATGTTGGGA GGACGAAAAT   241 GGTGACAAGA CAGATGGCCA GAAATACACC AACAGCACGC ATGATGTCTA CTGGGGATTT   301 ATTCTTTAGT GCGGGGGAAT ACACGGCTTT TAATACGATT GAGGGCGTCT CCTAACAAGT   361 TACATCACTC CTGCCCTTCC TCACCCTCAT CTCCATCACC TCCTTCATCT CCGTCATCTC   421 CGTCATCACC CTCCGCGGCA GCCCCTTCCA CCATAGGTGG AAACCAGGGA GGCAAATCTA   481 CTCCATCGTC AAAGCTGCAC ACAGTCACCC TGATATTGCA GGTAGGAGCG GGCTTTGTCA   541 TAACAAGGTC CTTAATCGCA TCCTTCAAAA CCTCAGCAAA TATATGAGTT TGTAAAAAGA   601 CCATGAAATA ACAGACAATG GACTCCCTTA GCGGGCCAGG TTGTGGGCCG GGTCCAGGGG   661 CCATTCCAAA GGGGAGACGA CTCAATGGTG TAAGACGACA TTGTGGAATA NCAAGGGCAG   721 TTCCTCGCCT TANGTTGTAA AGGGAGGTCT TANTACCTCC ATATACGANC ACACCGGCGA   781 NCCAAGTTCC TTCGTCNGTA NTCCTTTCTA CGTGACTCCT AGCCAGGANA GCTCTTAAAC   841 CTTCTGCAAT GNTCTCAAAT TTCNGNNTGG ANNCTNCTTG NNCACGATGC TTTCNNAACC   901 ANCCTCCTTT TTTGCNCCTG NNTNCATCAC CCTGANCCCG GGGNCNAGNG NNTGGGNCTT 961 CNNCNNNNNC ATCNGCGGGN CCNNNNNCTA TCGCTNCNNG GGGGCNCNNN NNNNNNN //
Primer: SV40pro_ori_F Region: SV40 pro_ori, NeoR_KanR Sequence file: RDB17727_A9Kcd.seq
>D06721A6_A9Kc_1_SV40pro_ori_F_D01_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNANN NNNNNCTCGN CCTCTGANCT ATTCNNGAAG TAGTGAGGAG    61 GCTTTTTTGG AGGCCTAGGC TTTTGCAAAG ATCATCAAGA GACAGGATGA GGATCGTTTC   121 GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT   181 TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT   241 CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC   301 TGCAAGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG   361 TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC   421 AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA   481 TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC   541 GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG   601 AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG AGCATGCCCG   661 ACGGCGANGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA   721 ATGGCCGCTT TTCTGGANTC NTCNNCTGTG GCCGGCTGGG TGTGGCGGAN CGCTATCANG   781 ANATANCGTT GGCTANCCGT GATATTGCTG AANAGCTTGN CGGCNAATGG GNCTGANCGC   841 TNCCNNGNGN TTNCNGTATC GNCGCTCCCG NNTTCNNAGC NGCNTCNNCN TTNNNATCGC   901 CTTCNTGANN NNNNNNTTCT GANCGGGNNN NNTNGGGGTT CGAANNNNNN NNANNNNNCN   961 ACGCCCNNNC NNGNNNNCNC NNNNANTTNC NNNTNCNNNC NCNNNCNTNN NNNNAANNNN 1021 NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.07.27

GNP_filter3_RDBDEP_html_220727.pl