Please review the QC test results indicated by icon below as well as clone information before placing your order.

pN1-OPA1(123Nt)-Halo

Halo Tag, Mitochondrial intermembrane space localization

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Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR (Wang et al, Proc. Natl. Acad. Sci. U S A, 116(32): 15817-15822, 2019) are requested. The BIOLOGICAL RESOURCE shall be transferred to a not-for profit organization. The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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提供条件	利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Wang et al, Wang et al, Proc. Natl. Acad. Sci. U S A, 116(32): 15817-15822, 2019) 。本件リソースは非営利機関の学術研究目的にのみ利用することができる。 付加的提供条件： 本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
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Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17747_A9L3p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro,insert 5'(OPA1(123Nt),Halo tag 5') Sequence file: RDB17747_A9L3a.seq
>D06362B8_A9L3_1_CMV_Forward_A03_03_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN TNNNNNCAAN GGGCGGTAGG CGTGTACGGT GGGAGGTCTA    61 TATAAGCAGA GCTGGTTTAG TGAACCGTCA GATCCGCTAG CCACCATGTG GCGACTACGT   121 CGGGCCGCTG TGGCCTGTGA GGTCTGCCAG TCTTTAGTGA AACACAGCTC TGGAATAAAA   181 GGAAGTTTAC CACTACAAAA ACTACATCTG GTTTCACGAA GCATTTATCA TTCACATCAT   241 CCTACCTTAA AGCTTCAACG ACCCCAATTA AGGACATCCT TTCAGCAGTT CTCTTCTCTG   301 ACAAACCTTC CTTTACGTAA ACTGAAATTC TCTCCAATTA AATATGGCTA CCAGCCTCGC   361 AGGAATTTTT GGCCAGCAAG ATTAGCTACG AGACTCTTAA AACTTCGCTA TCTCATACTA   421 GGATCGGCTG TTGGGGGTGG CTACACAGCC AAAAAGACTT TTGATCAGTG GAAAGGGGAT   481 CCACCGGTCT CGACCGGCAG CGAAATCGGT ACTGGCTTTC CATTCGACCC CCATTATGTG   541 GAAGTCCTGG GCGAGCGCAT GCACTACGTC GATGTTGGTC CGCGCGATGG CACCCCTGTG   601 CTGTTCCTGC ACGGTAACCC GACCTCCTCC TACGTGTGGC GCAACATCAT CCCGCATGTT   661 GCACCGACCC ATCGCTGCAT TGCTCCAGAC CTGATCGGTA TGGGCAAATC CGACAAACCA   721 GACCTGGGTT ATTTCTTCGA CGACCACGTC CGCTTCATGG ATGCCTTCAT CGAAGCCCNG   781 GGTCTGGAAG ANGTCGTCCT GGTCATTCAC GACTGGGGCT CCGCTCNGGG TTTCCACTGG   841 GCCNAGCGCA ATCCAGANCG CGTCNANNGT ANNGCATTTA TGGAGTTCAT CCGCCNNATN   901 CNNANNNNNN NGAANNGNCA GNANTTNNCC NNNNNNNNCT TNCNNNNNNT NCGNNNNNNN 961 NNN //
Primer: pAxEF R2 (Pr0059) Region: SV40 pA,insert 3'(Halo tag 3') Sequence file: RDB17747_A9L3b.seq
>D06362B8_A9L3_1_pAxEF R2_B03_06_ABI24.ab1     1 NNNNNNNNAN NGNNTNATTT GTGAANTTGT GNNGCTATTG CTTTATTTGT AACCATTATA    61 AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG   121 GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT GGCTGATTAT   181 GATCTAGAGT CGCGGCCGCT TAACCGGAAA TCTCCAGAGT AGACAGCCAG CGCGCGATCT   241 CGCTGCCGAT CAGGTCCGGG TTGTCTTCTT GCAGCAGATT CAGACCCGGG CCGATGTCCA   301 CAGCCTTGCA GTTAGGCAGG CTTTTGGCCA GGCGAGCGGC TTCGGCCGGT GGGATCAGAA   361 CGCCTGGGGT GCCCCAGAAC AGCAGCTTCG GGACAGGGGA CTGGTGCAGC CAGTCCATGT   421 ATTCTTCGAC CAGCGCGACG ATGTTCGCTG GCTCACCGGC GATTGGCAGC TCGTTTGGGA   481 AGCGCCACAG TGGCTCGCGG TCAACAGGAT TCAGGAACGG CTCGCGGTAA TGGTCCATCT   541 CGACTTCAGT CAGCGGGCGG ACGACACCCA TCGGCAGCGT ACCCTCGATA AAAACGTTCT   601 GATCGATGAT CAGCTTGCGG CCGACGTCGG TGGTGCGGAA GGCCTGGAAG GTCTCGCGGG   661 CAAATTCTGG CCATTCGTCC CAGGTCGGGA TAGGGCGGAT GAACTCCATA AATGCAATAC   721 CTTTGACGCG CTCTGGATTG CGCTTGGCCC AGTGGAAACC CAGANCGGAN CCCCAGTCGT   781 GAATGACCAG GACGANCTCT TCCAGANCCA NGGCTTCNAT GAANGCATCC NTGANNCGNA   841 NGTGGTCGTC NAAGAAATAA CCCANGNCNG GTTTGNCNGA NTTGCCCATA CCNNTCNNGN   901 CTGGANCAAN GCANCNATGG GNNNGNNCNN NATGCNGGNN NANGTNGCNN CNCNNNNNNN 961 NNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori, NeoR_KanR Sequence file: RDB17747_A9L3c.seq
>D06362B8_A9L3_1_SV40pro_ori_F_C03_09_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNC GCCTCGGCCT CTGNNCTATT CNNGAAGTAG TGAGGAGGCT    61 TTTTTGGAGG CCTAGGCTTT TGCAAAGATC GATCAAGAGA CAGGATGAGG ATCGTTTCGC   121 ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC   181 GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA   241 GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG   301 CAAGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG   361 CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG   421 GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG   481 CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC   541 ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA   601 GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGAG CATGCCCGAC   661 GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT   721 GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGANCG CTATCAGGAC   781 ATAGCGTTGG CTACNCGTGA TATTGCTGAA GANCTTGGCG GCGAATGGGC TGANCGCTTC   841 CTCGNGCTTT ANGGNATCGC CGCTCCCNAT TCNCAGCGCA TCGCCTNCNN TCGCCTNCTT   901 GNCNANTTCN TCTGANCGGG NNTNNGGGGN NTNNGAAANG ANCNNNNNAN NNNNNNCNNN 961 NCNNNNNNNN NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl