Please review the QC test results indicated by icon below as well as clone information before placing your order.

pN1-QUE37C

ATP measurement

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

The RECIPIENT acknowledges that the BIOLOGICAL RESOURCE contains the modification of genetically-encoded ratiometric fluorescent ATP indicator QUEEN made by the collaboration of Dr. Hiromi Imamura of the Kyoto University and Dr. Hideyuki Yaginuma of the University of Tokyo and modified by Dr. Hideyuki Yaginuma who moved to RIKEN and Dr. Yasushi Okada of RIKEN. The Kyoto University, the University of Tokyo, and RIKEN are the co-owner of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature (Yaginuma, H. and Okada, Y., (2021) bioRxiv https://doi.org/10.1101/2021.10.08.463131) designated by the DEPOSITOR and an acknowledgment to Dr. Yasushi Okada are requested. The BIOLOGICAL RESOURCE shall be transferred to a not-for profit organization. The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research. For for-profit-use of the BIOLOGICAL RESOURCE, the RECIPIENT shall contact the Kyoto University, Accounts Division Administration Office (Science), Main Campus (A20gaibushikin@mail2.adm.kyoto-u.ac.jp), the University of Tokyo Engineering and Information Science and Engineering Office, Finance Department, External Funding Team (t-pat@t-adm.t.u-tokyo.ac.jp) and the RIKEN Kobe Branch, General Affairs Section (kobe-ippr@riken.jp) in advance and should obtain a commercial license from all of them. Neither the Kyoto University, the University of Tokyo nor the RIKEN has obligation to grant a commercial license. RECIPIENT must comply with the terms and conditions of the AUTM Standard Material Transfer Agreement (for Biological Materials).

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提供条件

本件リソースは、京都大学の今村博臣博士並びに東京大学の柳沼秀幸博士が共同で開発したアデノシン3リン酸測定のための蛍光標識融合タンパク質QUEENをコードするプラスミドを理化学研究所に移籍した柳沼秀幸博士が岡田康志博士とともに改変した変異体であり、京都大学、東京大学、及び理化学研究所が共有するものである。利用者は、研究成果の公表にあたって寄託者が指定する文献 (Yaginuma, H. and Okada, Y., (2021) bioRxiv https://doi.org/10.1101/2021.10.08.463131) を引用し、謝辞に岡田康志博士を含めること。 本件リソースは非営利機関の学術研究目的にのみ利用することができる。利用者が営利目的利用を希望するときは、京都大学本部構内（理系）共通事務部 経理課 外部資金掛 (A20gaibushikin@mail2.adm.kyoto-u.ac.jp)、 東京大学工学系・情報理工学系等事務部 財務課 外部資金チーム (t-pat@t-adm.t.u-tokyo.ac.jp) 並びに理化学研究所神戸事業所総務課 (kobe-ippr@riken.jp) に連絡し、それぞれから別途の許諾を事前に受けるものとする。尚、京都大学、東京大学並びに理化学研究所はかかる許諾を行う義務を負うものではない。利用者は、AUTM Standard Material Transfer Agreement (for Biological Materials)に定める諸条件を遵守すること。

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提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17794_A9Lap1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro,insert 5' Sequence file: RDB17794_A9Laa.seq
>D06362H1_A9La_1_CMV_Forward_C01_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNNTGN NGCNNTGGGC GGTANGCGTG TACGGTGGGA NGTCTATATA    61 AGCAGAGCTG GTTTAGTGAA CCGTCAGATC CGCTAGCGCT ACCGGACTCA GATCTCGAGC   121 TCAAGCTTCG AATTCTGCAG TCGACGGTAC CGCGGGCCCG GGATCCACCG GTCGCCACCA   181 TGGTGAAAAC TGTGAAAGTG AATATAACAA CCCCTGATGG GCCAGTCTAC GACGCTGATA   241 TCGAGATGGT GTCCGTGCGG GCCGAGAGTG GTGATCTCGG CATCCTCCCC GGTCACATTC   301 CCACAAAGGC CCCACTGAAG ATCGGAGCTG TGCGGCTGAA GAAGGACGGC CAAACAGAGG   361 ATGTCGCAGT CTCAGGCGGC ACTGTTGAAG TGCGGCCTGA CCACGTTACC ATTAATGCTC   421 AAGCCGCTGA ACGGGCGGAG GACATTGACG TCCTCCGCGC CAAAGCGGCG AAAGAGCGGG   481 CGGAGCGCCG CCTGCAAAGC CAGACCCGTT ACAACAGCCA CAACGTCTAT ATCATGGCCG   541 ACAAGCAGAA GAACGGCATC AAGGTGAACT TCAAGATCCG CCACAACATC GAGGACGGCA   601 GCGTGCAGCT CGCCGACCAC TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC   661 TGCCCGACAA CCACTACCTG AGCACCCAGT CCAAGCTGAN CAAAGACCCC AACGAGAAGC   721 GCGATCACAT GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG GATCACTCTC GGCATGGACG   781 ANCTGTACAA GGGTGGCACC GGANGGNNCA TGGNGAGCAA GGGCGNNGAN CTGTTCACCG   841 GGGGNGGNGC CCATCCTGGN CGANCTGGAC GGCGANNNAA ACGGNCNCAA NTNCAGCGNG   901 NCCNGNNANG NNGANGGCGA TGCCNNNNAC NGNNNNCNNN NCNNNAANTN NNTNNNNNNN 961 NNN //
Primer: SV40polyA_R2 (Pr0623) Region: insert 3' Sequence file: RDB17794_A9Lab.seq
>D06362H1_A9La_1_SV40polyA-R2_C02_08_ABI24.ab1     1 NNNNNNNNNN CNNCANANNN ANGTTAACAN NNACNATTGC ATTCNTTTTA TGTTTCAGGT    61 TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTATGGC   121 TGATTATGAT CTAGAGTCGC GGCCGCTCAC TTATCTTCCG CAACGCTCAA ACGGTTCATG   181 GCGCGTTTTA ACGCCAGTTC GGCCCGTTTG AAGTCGATGT CACCCAGGTT GTACTCCAGC   241 TTGTGCCCCA GGATGTTGCC GTCCTCCTTG AAGTCGATGC CCTTCAGCTC GATGCGGTTC   301 ACCAGGGTGT CGCCCTCGAA CTTCACCTCG GCGCGGGTCT TGTAGTTGCC GTCGTCCTTG   361 AAGAAGATGG TGCGCTCCTG GACGTAGCCT TCGGGCATGG CGGACTTGAA GAAGTCGTGC   421 TGCTTCATGT GGTCGGGGTA GCGGCTGAAG CACTGCACGC CGTAGGTCAG GGTGGTCACG   481 AGGGTGGGCC AGGGCACGGG CAGCTTGCCG GTGGTGCAGA TGAACTTCAG GGTCAGCTTG   541 CCGTAGGTGG CATCGCCCTC GCCCTCGCCG GACACGCTGA ACTTGTGGCC GTTTACGTCG   601 CCGTCCAGCT CGACCAGGAT GGGCACCACC CCGGTGAACA GCTCCTCGCC CTTGCTCACC   661 ATGCTCCCTC CGGTGCCACC CTTGTACAGC TCGTCCATGC CGANAGTGAT CCCGGCGGCG   721 GTCACGAACT CCAGCAGGAN CATGTGATCG CGCTTCTCGT TGGGGTCTTT GCTCAGCTTG   781 NACTNNNTGC TCANGTAGNG GTTGTCGGGC AGCANCACGG GGCCGTCGCC NANGNNGGTG   841 TTCTGCTGGT ANNGGTCGGC GANCTGCACG CTGCCNNNCT CNATGNTGTG GCGGATCTNN 901 AAGTTCACCT NNNTGNCGTT CTNNNGCTNN NCNGNNNNNN NNNN //
Primer: QUEEN-M_R (Pr0808) Region: insert mid Sequence file: RDB17794_A9Lac.seq
>D06362H1_A9La_1_QUEEN-M_R_C07_03_ABI08.ab1     1 NNNNNNNNNN NNCNNNNNNN NNNNCNNGNN NNGCTGNACT TGTGGCCGTT TACGTCGCCG    61 TCCAGCTCGA CCAGGATGGG CACCACCCCG GTGAACAGCT CCTCGCCCTT GCTCACCATG   121 CTCCCTCCGG TGCCACCCTT GTACAGCTCG TCCATGCCGA GAGTGATCCC GGCGGCGGTC   181 ACGAACTCCA GCAGGACCAT GTGATCGCGC TTCTCGTTGG GGTCTTTGCT CAGCTTGGAC   241 TGGGTGCTCA GGTAGTGGTT GTCGGGCAGC AGCACGGGGC CGTCGCCGAT GGGGGTGTTC   301 TGCTGGTAGT GGTCGGCGAG CTGCACGCTG CCGTCCTCGA TGTTGTGGCG GATCTTGAAG   361 TTCACCTTGA TGCCGTTCTT CTGCTTGTCG GCCATGATAT AGACGTTGTG GCTGTTGTAA   421 CGGGTCTGGC TTTGCAGGCG GCGCTCCGCC CGCTCTTTCG CCGCTTTGGC GCGGAGGACG   481 TCAATGTCCT CCGCCCGTTC AGCGGCTTGA GCATTAATGG TAACGTGGTC AGGCCGCACT   541 TCAACAGTGC CGCCTGAGAC TGCGACATCC TCTGTTTGGC CGTCCTTCTT CAGCCGCACA   601 GCTCCGATCT TCAGTGGGGC CTTTGTGGGA ATGTGACCGG GGAGGATGCC GAGATCACCA   661 CTCTCGGCCC GCACGGACAC CATCTCGATA TCAGCGTCGT AGACTGGCCC ATCAGGGGTT   721 GTTATATTNN NTTTCACAGT TTTCACCATG GTGGCGACCG GTGGATCCCG GGCCCGCGGT   781 ACCGTCGACT GCAGAATTCG AAGCTTGAGC TCGAGATCTG AGTCCGGTAN CGCTAGCGGA   841 TCTGACNGTT CANTANNCCA GCTCTGCTTA TATAGACCTC CCNNCGTANA CGCCTACCGC   901 CCNTTTGCGT CNATGGNNNG GANTTGTTAC NACATTTTGG AAAGNNCCGT TGANTTTGGT   961 GNCNAAACAA ACTNNNANNG NNGTNNATGG GGNGGANNNN NNGNAAANNC CCNNGANNNA 1021 NNNNCTNTNC NNNNCCNNNG NNNNANNNNN NAANNNNNNN NNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,NeoR_KanR Sequence file: RDB17794_A9Lad.seq
>D06362H1_A9La_1_SV40pro_ori_F_C03_09_ABI24.ab1     1 NNNNNNNNNN NNGNNGCCTC NGCCTCTGNN CTATTCNNGA AGTAGTGAGG AGGCTTTTTT    61 GGAGGCCTAG GCTTTTGCAA AGATCGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT   121 TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA   181 TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA   241 GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAAGA   301 CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA   361 CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT   421 CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG   481 GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA   541 GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA   601 TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGAGCATGC CCGACGGCGA   661 GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG   721 CTTTTCTGGA TTCATCGACT GNNNCCGGCT GGGTGTGGCG GANCGCTATC ANGACATAGC   781 GTTGGCTACC CGTGATATTG CTGANNANCT TGGCGGCGAA TNNNCTGANC GCTTCCTCGN   841 GCNNNACNGT ATCGCCGCNC CCGATTCNCA NCGCATCGCC TTCTATCGCC NTCNNNANGA   901 NTNCTTCTNN NCGGGNNNCN NGGGNNNCNA AANGANCNAN CNANNNNCNN CCNANCNNNN 961 NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.03.26

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