Please review the QC test results indicated by icon below as well as clone information before placing your order.

spret MACS1-luc2

Luciferase reporter of the Western Mediterranean mouse (Mus spretus) Shh enhancer, MACS1.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Tsukiji N. et al., Mech. Dev. 131:127-36, 2014)
Additional terms and conditions for distribution	The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Tsukiji N. et al., Mech. Dev. 131:127-36, 2014)。
付加的提供条件	本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17897_B0Afp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4ins_F Region: Pause site, Spret MACS1 5' Sequence file: RDB17897_B0Afa.seq
>D06932A2_B0Af_2_pGL4ins_F_D10_10_ABI24.ab1     1 NNNNNNNNNN NNNNNNACNN GCNNATAGGC TGTCCCCNGT GCAAGTGCAG GTGCCAGAAC    61 ATTTCTCTGG CCTAACTGGC CGGTACCTGA GCTCGCTAGC CTCGAGATAA CCAGGAGTCC   121 AGGTACTTGA TATGTAAAGC AATTAGTGGT CATTTGAAAA CAGTACACTG GCTAAAAGTT   181 AAAAAAATAC TAACAAAAGC ATTTAGGTGA ACTCTGTCTG CTCAGGAGAG TCCTAAGTGA   241 TCCATCTAAT GTCAGCAAAT TGCTTACTAA TGTGTTGACT TAGGGCCACC CTCAAAAGCA   301 AAATGAAAAG CCTGGAATTT ATAGCATCTC AATGCATCAG CTGATAAGCT TTTACTGGTT   361 TAATATCCTT CCAGGCTTGC AGCTTGGAGC CACTTCAAGT TAGCAACAGA ATGTTTGCCA   421 TCTCTATGTG ATAAAGGAAG TCAAAGGGAT GAACAAACTT CAGGACCATT ATGCTGTTTG   481 GTGAGATGGA AATGAAAAAG CTAGAGAAGT CACGGGTTGG GGGTGTGTGA GAGCTGGGCC   541 TTCAAGTCCG GCCTCTGACG GATTGTTAGC CTCACCCCTC AAACAATGGT CTGGACTTTA   601 GCTGGAGCAG AGATAACATA GTGCATTTGG AAAGTTTTAA TTGCAGCAAT AAAAGACTAT   661 GCCTCAATTA TAGGACAGAT GCTAGCAAAG TTTCCAGATA AGGATCAAAG GCACCTAAGG   721 AGTGGCCACT GCACTTTGGG ACACACTAGG CTTTCTGTAC ACACTGCTGG GGAATTAGCA   781 CATCAGCTTT CCGACCTTTC TAGGTCCTTT GATCTGAGTT CCCAAGCAAG TAAGTGTATC   841 AAGANTACTG TATCAGCCAG ACAGGTCCNA CACTCCCTTG CTGAATTTNN ATCCTGANAC   901 TCACNAGNAC AAAACCNAAC ACCTGANCTT NNCAGTTCNN ANCACNAAAG TTCAGNGNGC 961 TANNNCNNGC CNCNGNGGNC NANCTNANAN NCNNNAGGGN ATNNNNNNNN NN //
Primer: Luc_R Region: luc2 5', mini P, Spret MACS1 3' Sequence file: RDB17897_B0Afb.seq
>D06932A2_B0Af_2_Luc_R_E10_13_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN GNAGCGCTTC NTGGCTTTGT GCNGCTGCTC GCCGGCGGTC    61 CCGTCTTCGA GTGGGTAGAA TGGCGCTGGG CCCTTCTTAA TGTTTTTGGC ATCTTCCATG   121 GTGGCTTTAC CAACAGTACC GGATTGCCAA GCTGGAAGTC GAGCTTCCAT TATATACCCT   181 CTAGTGTCTA AGCTTGGCCG CCGAGGCCAG ATCTAGCACA CTGAACTTTT GTGGTAAGAA   241 CTGCAAAGGT CAGGTGTTAG GTTTTGTACT GGTGAGTGTC AGGATTAAAA TTCAGCAAGG   301 GAGTGTTGGA CCTGTCTGGC TGATACAGTA GTCTTGATAC ACTTACTTGC TTGGGAACTC   361 AGATCAAAGG ACCTAGAAAG GTCGGAAAGC TGATGTGCTA ATTCCCCAGC AGTGTGTACA   421 GAAAGCCTAG TGTGTCCCAA AGTGCAGTGG CCACTCCTTA GGTGCCTTTG ATCCTTATCT   481 GGAAACTTTG CTAGCATCTG TCCTATAATT GAGGCATAGT CTTTTATTGC TGCAATTAAA   541 ACTTTCCAAA TGCACTATGT TATCTCTGCT CCAGCTAAAG TCCAGACCAT TGTTTGAGGG   601 GTGAGGCTAA CAATCCGTCA GAGGCCGGAC TTGAAGGCCC AGCTCTCACA CACCCCCAAC   661 CCGTGACTTC TCTAGCTTTT TCATTTCCAT CTCACCAAAC AGCATAATGG TCCTGAAGTT   721 TGTTCATCCC TTTGACTTCC TTTATCACAT AGAGATGGCA AACATTCTGT TGCTAACTTG   781 AAGTGGCTCC NAGCTGCAAG CCTGGAANGA TATTAAACCA GTAAAAGCTT ATCAGCTGAN   841 GCATTGAGAT GCTATAAATT CCAGGCTTTT CATTTTGCTT TTGANGGTGG NCCTAANTCA   901 ACACATTAGN AAGCAATTTG CTGANNTTAN NNGGNTCACT NANGANTCTC CTGANCANAN   961 AGANTTCNNC TAANNGNTTT TGNTANTATT TTTTTNNCTT TTANCCNGNG NNNNGNTTTN 1021 NNNGANNNCT NNNNGNNTNN CNNNNNNNNN N //
Primer: pGL4pA_R Region: SV40 late p(A), luc2 3' Sequence file: RDB17897_B0Afc.seq
>D06932A2_B0Af_2_pGL4pA_R_F10_16_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNC NTNNTCGANT TTNCNNNNTT TGTAGAGGTT TTACTTGCTT    61 TAAAAAACCT CCCACACCTC CCCCTGAACC TGAAACATAA AATGAATGCA ATTGTTGTTG   121 TTAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA   181 CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT   241 CTTATCATGT CTGCTCGAAG CGGCCGGCCG CCCCGACTCT AGAATTATTA CACGGCGATC   301 TTGCCGCCCT TCTTGGCCTT AATGAGAATC TCGCGGATCT TGCGGGCGTC CAACTTGCCG   361 GTCAGTCCTT TAGGCACCTC GTCCACGAAC ACAACACCAC CGCGCAGCTT CTTGGCGGTT   421 GTAACCTGGC TGGCCACATA GTCCACGATC TCCTTCTCGG TCATGGTTTT ACCGTGTTCC   481 AGCACGACGA CTGCGGCGGG CAGCTCGCCG GCATCGTCGT CGGGCAGGCC GGCGACCCCG   541 GCGTCGAAGA TGTTGGGGTG TTGCAGCAGG ATGCTCTCCA GTTCGGCTGG GGCTACCTGG   601 TAGCCCTTGT ATTTGATCAG GCTCTTCAGC CGGTCCACGA TGAAGAAGTG CTCGTCCTCG   661 TCCCAGTAGG CGATGTCGCC GCTGTGCAGC CAGCCGTCCT TGTCGATGAG AGCGTTTGTA   721 GCCTCGGGGT TGTTAACGTA GCCGCTCATG ATCATGGGGC CACGGACGCA CAGCTCGCCG   781 CGCTGGTTCA CACCCAGTGT CTTACCGGTG TCCAAGTCCA CCACCTTAGC CTCGAANANN   841 GGCACCACCT TGCCNACTGC GCCAGGCTTG TCGTCCCNTT CNGGGGNGAN NANANNGNNN   901 CNNNNTGNTN CTGNCANGNN GTANCCCTGN CGGANGNCTG GNNNGNGNNA NCGTTTGGNN   961 NNNNGNNNTC NNNTNNNNNC NNTGNNNANC NGNGCCCCGN NNNNNGNNGA NNNNNNGNNN 1021 GTNGNNTNNN NTCNNANNTN NNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl