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rat MACS1-luc2

Luciferase reporter of the ACI/N rat Shh enhancer, MACS1.

Catalog number RDB17898
Resource name rat MACS1-luc2
Clone info. Luciferase reporter of the ACI/N rat Sonic hedgehog (Shh) enhancer, MACS1.
Comment Expression was confirmed by the depositor with luciferase assay.
Vector backbone pGL4.23 (plasmid)
Size of vector backbone 4.3 kb
Selectable markers Ampicillin
Gene/insert name rat Shh genomic
Depositor|Developer Amano, Takanori |
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External Database
rat Shh

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Tsukiji N. et al., Mech. Dev. 131:127-36, 2014)
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Tsukiji N. et al., Mech. Dev. 131:127-36, 2014)。
          付加的提供条件 本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
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          RDB17898 rat MACS1-luc2 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Tsukiji, N., A novel regulatory element for Shh expression in the lung and gut of mouse embryos. Mech. Dev. 131: 127-136 (2014). PMID 24157522.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB17898_B0Afp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4ins_F
          Region: Pause site, rat MACS1 5'
          Sequence file: RDB17898_B0Afa.seq check
          >D06932A3_B0Af_2_pGL4ins_F_G10_19_ABI24.ab1
              1 NNNNNNNNNN NNNNNCNNGC NNATAGGCTG TCCCCAGTGC AAGTGCAGGT GCCAGAACAT
             61 TTCTCTGGCC TAACTGGCCG GTACCTGAGC TCGCTAGCCT CGAGATAACC AGGAGTCCAG
            121 GTACTTGATA TGCAAAGCAA TACTAGTCAT TTGAAAACAG TACACTGGCT AAATGTTAAA
            181 AAAAAAAAAC AACAAAAGCA TTTAGGTGAA TTCTGTCTGC TCAGGAAAGT CTTAAGTGAT
            241 CCATCTCATG TCAGCAAATT GCTTACTAAT GTGTTGACTT AGGGCCACTC TCAAAAGCAA
            301 AAAGAAAAGC CTGGAATTTA TAGCATCTCA ATGCATCAGC TGATAAGCTT TTACTGGTTT
            361 AATATGCTTC CAGGCTTGCA GCCTGGAGCC ACTTCAAGTT AGCAACAGAA TGTTTGCCAT
            421 CTCCATGTGA TAAAGGAAGT CAAAGGGATG AACAAACTTC AGGACCATTA TGCTGTTTGG
            481 TGAGATGGAA ATGAAAAACT AGAGAAGTCA CAGGTTTGGG GGTGTGTGAG AGCTGGGCCT
            541 TCAAGTCCAG CATCTGACGG ATTGTTAGCC TCACTTCTCA AACAATGGTC TGGACTTTAG
            601 CTGGGGCAGA GATAACATAG TGCATTTGAA AAGTTTTAAT TGCAGCAATA AAAGACCATG
            661 CCTCAATTAT ATGACAGATG CTAGCAAAGT TTCCAGATAA GGATCAAAGC CATCCNAGGA
            721 GTGNNCACTG CTCTTTGGGC CACACTAGGC TTTCTGTACA CACTGCTGGG GAATTAGCAC
            781 AGCAGCTTTT CCNANCTTTT TTCTTCCTTT GATGTGAGTT CCTAAGCNAG TTAAGTGTAT
            841 CAAGACTACN GTATCAGCCA GANNGGTCCN ACACTCCNNT GCTGAATTTT AATCCTGANA
            901 CTCNACAGTA CAAAANCNAA TACNNGANNN TTGCAGTTCT TANCNCAAAA GTNCANNGNG
            961 NTANNTNNNG NNNNGGCGGN NNAGCTNNNA NNNNANANGG NANANNNNGN NNNNNNGANT
          1021 NNNNNCNNNN NNNNNN
          //
          Primer: Luc_R
          Region: luc2 5', rat MACS1 3'
          Sequence file: RDB17898_B0Afb.seq check
          >D06932A3_B0Af_2_Luc_R_H10_22_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN GNANCGCTTN NTGGCTTTGT GCNGCTGCTC GCCGGCGGTC
             61 CCGTCTTCGA GTGGGTAGAA TGGCGCTGGG CCCTTCTTAA TGTTTTTGGC ATCTTCCATG
            121 GTGGCTTTAC CAACAGTACC GGATTGCCAA GCTGGAAGTC GAGCTTCCAT TATATACCCT
            181 CTAGTGTCTA AGCTTGGCCG CCGAGGCCAG ATCTAGCACA CTGAACTTTT GTGGTAAGAA
            241 CTGCAAAGGT CAGGTATTAG GTTTTGTACT GTTGAGTGTC AGGATTAAAA TTCAGCAAGG
            301 GAGTGTTGGA CCTGTCTGGC TGATACAGTA GTCTTGATAC ACTTAACTTG CTTAGGAACT
            361 CACATCAAAG GAAGAAAAAA GGTCGGAAAA GCTGCTGTGC TAATTCCCCA GCAGTGTGTA
            421 CAGAAAGCCT AGTGTGGCCC AAAGAGCAGT GGCCACTCCT TGGATGGCTT TGATCCTTAT
            481 CTGGAAACTT TGCTAGCATC TGTCATATAA TTGAGGCATG GTCTTTTATT GCTGCAATTA
            541 AAACTTTTCA AATGCACTAT GTTATCTCTG CCCCAGCTAA AGTCCAGACC ATTGTTTGAG
            601 AAGTGAGGCT AACAATCCGT CAGATGCTGG ACTTGAAGGC CCAGCTCTCA CACACCCCCA
            661 AACCTGTGAC TTCTCTAGTT TTTCATTTCC ATCTCACCAA ACAGCATAAT GGTCCTGAAG
            721 TTTGTTCATC CCTTTGACTT CCTTTATCAC ATGGAGATGG CAAACATTCT GTTGCTAACT
            781 TGAAGTGGCT CCNGGCTGCA AGCCTGGAAG CATATTANAC CAGTAAAAGC TTATCAGCTG
            841 ANGCATTGAG ATGCTATAAA TTCCAGGCTT TTCTTTTTGC TTTTGANANT GGNCCTAANN
            901 CNACACATTA NTAAGCAATT TGCTGANNTN AGANGNNTCA CTNNANANTT TCCTGANCAN
            961 ANAGANNNCA CCNAAANGCT TTNGNNGNTT TTTTTTTTTN NNNTTTANCN NGNNNNNNNN
          1021 TTTNNAANNN NNNNNNN
          //
          Primer: pGL4pA_R
          Region: SV40 late p(A), luc2 3'
          Sequence file: RDB17898_B0Afc.seq check
          >D06932A3_B0Af_2_pGL4pA_R_A11_02_ABI24.ab1
              1 NNNNNNNNNN NNNGNNCCTT ATCGANTTNA CNNCNTTTGT AGAGGTTTTA CTTGCTTTAA
             61 AAAACCTCCC ACACCTCCCC CTGAACCTGA AACATAAAAT GAATGCAATT GTTGTTGTTA
            121 ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA
            181 ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT
            241 ATCATGTCTG CTCGAAGCGG CCGGCCGCCC CGACTCTAGA ATTATTACAC GGCGATCTTG
            301 CCGCCCTTCT TGGCCTTAAT GAGAATCTCG CGGATCTTGC GGGCGTCCAA CTTGCCGGTC
            361 AGTCCTTTAG GCACCTCGTC CACGAACACA ACACCACCGC GCAGCTTCTT GGCGGTTGTA
            421 ACCTGGCTGG CCACATAGTC CACGATCTCC TTCTCGGTCA TGGTTTTACC GTGTTCCAGC
            481 ACGACGACTG CGGCGGGCAG CTCGCCGGCA TCGTCGTCGG GCAGGCCGGC GACCCCGGCG
            541 TCGAAGATGT TGGGGTGTTG CAGCAGGATG CTCTCCAGTT CGGCTGGGGC TACCTGGTAG
            601 CCCTTGTATT TGATCAGGCT CTTCAGCCGG TCCACGATGA AGAAGTGCTC GTCCTCGTCC
            661 CAGTAGGCGA TGTCGCCGCT GTGCAGCCAG CCGTCCTTGT CGATGAGAGC GTTTGTAGCC
            721 TCGGGGTTGT TAACGTAGCC GCTCATGATC ATGGGGCCAC GGACGCACAG CTCGCCGCGC
            781 TGGTTCACAC CCAGTGTCTT ACCGGTGTCC AAGTCCACCA CCTTAGCCTC GAANAANGGC
            841 ACCACCTTGC CNACTGCGCC NGGCTNGTCG TCCCCTTCNG GGGTGANCAN ANNGGCGCTG
            901 GTTGTTNCTG TCNNGNCGNA NCCCNGGCGG ANGNCNNNNN NNNGGAAGCG NTNGGNCNCG
            961 GCCTCNCNNA CCTCCTTGCT GANCGGNGCC CNGNNNCNNG NGATNNNNNG NNNNTNGCTN
          1021 NNNNNGNANN NNNNNNNGAN NNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Tsukiji, N., A novel regulatory element for Shh expression in the lung and gut of mouse embryos. Mech. Dev. 131: 127-136 (2014). PMID 24157522.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl