Please review the QC test results indicated by icon below as well as clone information before placing your order.

rabbit MACS1-luc2

Luciferase reporter of the rabbit Shh enhancer, MACS1.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Tsukiji N. et al., Mech. Dev. 131:127-36, 2014)
Additional terms and conditions for distribution	The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Tsukiji N. et al., Mech. Dev. 131:127-36, 2014)。
付加的提供条件	本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17899_B0Afp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4ins_F Region: Pause site , rabbit MACS1 5' Sequence file: RDB17899_B0Afa.seq
>D06932A4_B0Af_1_pGL4ins_F_B11_05_ABI24.ab1     1 NNNNNNNNNN NNNNNNGNNN NNAGGCTGTC CCCNGTGCAA GTGCAGGTGC CAGAACATTT    61 CTCTGGCCTA ACTGGCCGGT ACCTGAGCTC GCTAGCCTCG AGATTCCCTC CCTTTTTATG   121 TGATGGACAC CAGATGACCT GAGTAACGGC AGGGCAAGAC CCAGGAGAAA CAAATGACAA   181 CCGAGCTCTC GTTTTTAAAC TAATGGTTAA TACCACGAAT CCTACAGAAA CAGACGGAAA   241 TAAAATTTCC AATTTGAAAA AAGAGCAAGT GTCACTGTAT TTCTCTCTTA AGTCTCCTGT   301 TTCAGACACC TGATGAATTA AAGTAACTTG AGAGTCTGGG CTATTGATAT GTAAAGCGAT   361 TACTGGTCAT TTACAAGCAA CATACTGGCT AACCATTAAA AAAAAATACT AACAGAAGTA   421 CCCAGGTGAA CTTTGTCTAC CCAAGAGGCC TCTGTGAGTG ATCCATCTGG ATGTCAGCAA   481 ATTGCTTACT AATGTGTTGA CTTATGGCTG CCCTCAAAAG CAAAATGAAA AGCCTGGAAT   541 TTACAGCATA TCAATGAATC AGTTGATAAG CTTTTACTGG TTTAATATCC TTCCAGTCTT   601 GCTGCTTGGA GCCACTTCAA GTTAGCAACA GAATGTTTGC CATCTCCACG TGATAAAGGA   661 AGTCAAAGGA CTAAACAAAC TTTCAGACCA TTATGCTGTT TGGTGAGATG TAAATGAAAA   721 AAGTAAGAGA AACCCCAGGC CTGANGGTGT GGGANCGGGC AGCCCCTANG CTGACAAATC   781 ACTAACCTCA CTTTTNAAAC AACGGCCAGG ANTTTTAGCT GGGGTGGAGA TAATATAGTG   841 CATTTGAAAA GTTTTNATTG CAGCAATAAA AGANCATACC TCAATTATAT GACATATGCT   901 AGCAGAATTT TNANATAANG ANNAAAGGNN NTCNAGGANC NAGCACNGCA TTCTGGGNNA   961 CACTNNGGCT TTCTGNACNC NCNNGNNNNN GNNANNANCA TNNNAGCNNN GNNGNNNNTT 1021 CNNNNNNNNT NNNNNNNNNT TNNNNNAANC NNNNNNNNNN //
Primer: rabbit MACS1_F1 Region: rabbit MACS1 3' Sequence file: RDB17899_B0Afb.seq
>D06932A4_B0Af_1_rabbit MACS_F1_H08_08_ABI08.ab1     1 NNNNNNNNNN NNNNGNNNNG NAANNGAAAA NNNNAAGAGA AACCCCAGGC CTGAGGGTGT    61 GGGAGCGGGC AGCCCCTAGG CTGACAAATC ACTAACCTCA CTTTTTAAAC AACGGCCAGG   121 ACTTTTAGCT GGGGTGGAGA TAATATAGTG CATTTGAAAA GTTTTAATTG CAGCAATAAA   181 AGACCATACC TCAATTATAT GACATATGCT AGCAGAATTT TTAGATAAGG ATCAAAGGCA   241 TTCAAGGACC AAGCACTGCA TTCTGGGCAA CACTTGGGCT TTCTGTACAC ACTGGTGCCG   301 GGTATTAGCA TAACAGCTCT GCTGACCTTT CAGGGCCTCT TTGATCTGAG TTTGTCAAAA   361 CCAGAATGAA TGTGCACTGG GACTACTGTA TCATCCCCAG ACAAGTCAAA TACCCCCTGT   421 GACAAAGGCC TTGCTGACTG CATTTTCATT CTCTGACACT CACTCAAGGG GACACAACCC   481 AACATCTGAC CTCTCAGTTC TTGCCACAAG ACTGGCATAT GGAAAAGAAC CTCGTTTGTA   541 ACCTGACTTC GCTTTGTGAG GATACGTACG CCAGGACTTT AAAGCAAGGC ACCTTTGTGA   601 AAAGAAACCC TCCATGTATG GAAGTAGTCC TATTTGTAGA AAGATGATCC CTGCGTTTCA   661 TTAATTAGTT AACCCCCTAG TAGATGCGTG AAAAACAAAG ATCTGGCCTC GGCGGCCAAG   721 CTTAGACACT AGAGGGTATA TAATGGAAGC TCGACTTCCA GCTTGGCAAT CCGGTACTGT   781 TGGTAAAGCC ACCATGGAAG ANGCCNAAAA CATTAAGAAN GGCCCAGCGN CNTTCTACCN   841 ACTCGAANAN NGGNNCNCCN GCGANCAGCT GCNCNAAGCC NTGAAGCGNT ACGCCCNNNN   901 NCCNGGNNCC NTCGCCNTNN NNGACGNNCA TATCGAGGNN NNNNTNACNN ACNNNNNNNN 961 CTTNNNNATN ANCNNNNNNN NNGNNNNNNN NNN //
Primer: Luc_R Region: luc2 5', mini P, rabbit MACS1 3' Sequence file: RDB17899_B0Afc.seq
>D06932A4_B0Af_1_Luc_R_C11_08_ABI24.ab1     1 NNNNNNNNNN GNNNNNNNNN NNGNANCGCT TCNTGGCTTT GTGCNGCTGC TCGCCGGCGG    61 TCCCGTCTTC GAGTGGGTAG AATGGCGCTG GGCCCTTCTT AATGTTTTTG GCATCTTCCA   121 TGGTGGCTTT ACCAACAGTA CCGGATTGCC AAGCTGGAAG TCGAGCTTCC ATTATATACC   181 CTCTAGTGTC TAAGCTTGGC CGCCGAGGCC AGATCTTTGT TTTTCACGCA TCTACTAGGG   241 GGTTAACTAA TTAATGAAAC GCAGGGATCA TCTTTCTACA AATAGGACTA CTTCCATACA   301 TGGAGGGTTT CTTTTCACAA AGGTGCCTTG CTTTAAAGTC CTGGCGTACG TATCCTCACA   361 AAGCGAAGTC AGGTTACAAA CGAGGTTCTT TTCCATATGC CAGTCTTGTG GCAAGAACTG   421 AGAGGTCAGA TGTTGGGTTG TGTCCCCTTG AGTGAGTGTC AGAGAATGAA AATGCAGTCA   481 GCAAGGCCTT TGTCACAGGG GGTATTTGAC TTGTCTGGGG ATGATACAGT AGTCCCAGTG   541 CACATTCATT CTGGTTTTGA CAAACTCAGA TCAAAGAGGC CCTGAAAGGT CAGCANANCT   601 GTTATGCTAA TACCCGGCAC CAGTGTGTAC AGAAAGCCCA AGTGTTGNCN ANAANGCAGN   661 GNTNGGTCCT TGAATGNCTT TGATCCNTAT CTNAAAATTC TGNNNNNNNA TGTNNNATAA 721 TTGAGGNNNN GNNNNTTNNN NGNNNNNNTT NAAANTTTTC NAANGNNNNN NNNNN //
Primer: pGL4pA_R Region: SV40 late p(A), luc2 3' Sequence file: RDB17899_B0Afd.seq
>D06932A4_B0Af_1_pGL4pA_R_D11_11_ABI24.ab1     1 NNNNNNNNNN NNGGNNNNTT ATCGANTTTN CNNNNTTTGT AGAGGTTTTA CTTGCTTTAA    61 AAAACCTCCC ACACCTCCCC CTGAACCTGA AACATAAAAT GAATGCAATT GTTGTTGTTA   121 ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA   181 ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT   241 ATCATGTCTG CTCGAAGCGG CCGGCCGCCC CGACTCTAGA ATTATTACAC GGCGATCTTG   301 CCGCCCTTCT TGGCCTTAAT GAGAATCTCG CGGATCTTGC GGGCGTCCAA CTTGCCGGTC   361 AGTCCTTTAG GCACCTCGTC CACGAACACA ACACCACCGC GCAGCTTCTT GGCGGTTGTA   421 ACCTGGCTGG CCACATAGTC CACGATCTCC TTCTCGGTCA TGGTTTTACC GTGTTCCAGC   481 ACGACGACNG CGGCGGGCAG CTCGCCGGCA TCGTCGTCGG GCAGGCCGGC GACCCCGGCG   541 TCGAAGATGT TGGGGTGTTG CAGCAGGATG CTCTCCAGTT CGGCTGGGGC TACCTGGTAG   601 CCCTTGTATT TGATCAGGCT CTTCAGCCGG TCCACGATGA AGAAGTGCTC GTCCTCGTCC   661 CAGTAGGCGA TGTCGCCGCT GTGCAGCCAG CCGTCCTTGT CGATGAGAGC GTTTGTAGCC   721 TCGGGGTTGT TAACGTAGCC GCTCATGATC ATGGGGCCAC GGACGCACAG CTCGCCGCGC   781 TGGTTCACAC CCAGTGTCTT ACCGGTGTCC NAGTCCACCA CCTTANCCTC NAAGAANGGC   841 ACCACCTTGC CTACTGCGCC AGGCTNGTCG TCCCCTTCNG GGGTGANCAG AANGGCGCTG   901 GTTGNTTCTG TCNNGNCGTA NCCCNGGCGG ANGNCNGGNN NNGGAANCGN TNNNNCNCGG   961 NCTCNCCNNC CNNCTTGCTG ANCGGNGCCN NNNNNCTGGN NATNNNGNGN NANTNGNNNN 1021 NNNNNGNANN NNNNNATNAN NNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl