Please review the QC test results indicated by icon below as well as clone information before placing your order.

spret SLGE-luc2

Luciferase reporter of the Western Mediterranean mouse (Mus spretus) Shh enhancer, SLGE.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Tsukiji N. et al., Mech. Dev. 131:127-36, 2014)
Additional terms and conditions for distribution	The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Tsukiji N. et al., Mech. Dev. 131:127-36, 2014)。
付加的提供条件	本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17901_B0Afp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4ins_F Region: Pause site, spret SLGE 5' Sequence file: RDB17901_B0Afa.seq
>D06932A6_B0Af_2_pGL4ins_F_H11_23_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNGGCTG TCCCCNGTGC AAGTGCAGGT GCCAGAACAT    61 TTCTCTGGCC TAACTGGCCG GTACCTGAGC TCGCTAGCCT CGAGGCCAAG GAGGACTATG   121 TTTATTTAGA TGAGAAGAGG AAGACGTGCT GGGCATCAGT GCATTCTGTG TGTTTCAGGC   181 ATGACTGTGG TGTATAATGG TGTGTAGAAC ACTGCAAGAC ACTTAGAACA GAGAATGGTT   241 ATTAAAGGTT ATAGAAATGC TAATTATCTT GACTTGATCC TCAAATGTTT TCATGCACCA   301 AAACTTGTCT CTTAAGTGCA TAAAAACATT ATAAAATAAT AATAATAAAA ATAATAGAAG   361 TCAGCCACCC TCATTGCTGC CACCACAAGC ACATTCATCT CCTAGACCTC CTCAGCACTG   421 AAAACTTCTC CTCCTCCCTT CCCCCTTCTC CCCATCTTCT TCTCCTTCTC TTCCTCCTCC   481 CCTTCCTCCC CTTCCTCCTC CTCCTCCACT ACAACCTTCC TATGATCAGT GCTTCTTGCA   541 AGTAATCTGG CCTAAATGTA GGTGTCTCTT GCCCTTCTAG AATGTGCTCT TCAGTACAGA   601 ATGACTTAGG CCATTGTGCC CACATGGATG CTGTAGAAAG TTTCTACACA GTGGAGAAAG   661 CTCTAGATTA AGCACCCCTC TTTTGGAAGA ACCATCTGGA AAGGTCATGT GCCAGGCCAC   721 TGGAGATCAC TCAAGCCTCA CAGGCCCAGC AGCTCTTCCT GAGAACCTGT TGTGCTACAC   781 GGGGACAACT CTGCTGCAGC CCATTTCTGC AGTGTGTCCT TGGGCCAGCA CTCCCCTCTC   841 ACATTCCTCA GTCTTTGCTG GTGCACTTTC ATGTTTGNCN AANTCCNAGG NCTTCCTCCC   901 TCCCCTCTNN NCTTTGTCNN NNNGGTTCCC ANAANNGNCN ACTTCCCNNN CCCNCCCCCN   961 CCNNNNCNNN GGNNTNNNNN NNNCCCNNCN NNNNNCNNNN NCNNNNNNNG NTANCNCAGC 1021 TNCNGGGGNA NNNNNNANNN NNTNNNNNNN CNNNNNNNNN N //
Primer: mSLGE_F1 Region: spret SLGE Sequence file: RDB17901_B0Afb.seq
>D06932A6_B0Af_2_mSLGE_F1_F08_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNGCC NCNNNGGCCN NGCAGCTCTT CCTGAGAANC TGTTGTGCTA    61 CACGGGGNCA ACTCTGCTGC AGCCCATTTC TGCAGTGTGT CCTTGGGCCA GCACTCCCCT   121 CTCACATTCC TCAGTCTTTG CTGGTGCACT TTCATGTTTG CCTAACTCCA AGGCCTTCCT   181 CCCTCCCCTC TTGGCTTTGT CTTAAGGGTT CCCAGAAGGT CAACTTCCCT TGCCCTCCCC   241 CACCTCTCCA GTGGAGTAGG CACAACCCCA CTGATAACAC ATCCTTCTAT GTTAGCACAG   301 CTCCAGGGGC AAAGAGCAAA GGCTTAACCA CCCAAACAAC TCTGGGTGAG CAAACTCTGC   361 ACTAAAGACA TTCAGGGGCC AAAGACCTTC CAGGCTCAGA GCCACTCATT GAGGAGTGGA   421 GATAAAACAG TCAGTCGGGG TCCCAGCCAC TGCATGGGAT TCTTTGTATC TCACAGTTGG   481 CCCCAGAAAG GCCCCTTTAT AGAGGGCCAG GGCTGTATTA AAGGCAAGGA AAAAATGTCT   541 CTCAGCCCCC AAAAGTAAAC CACTCATGAA AGTACAGAAT TTTACAGGGG GATTTACTTC   601 CTTCTTATCT CCACAGAGGA AGGGAAGGCA AGGCTTGGGC AGGCAGACAG GTGCTGCATT   661 CCTCAGAGAA GCAGGTGGGG AGTCTGTAGC ACTGGTGCCG AGCCGCCTCC CACCCTGCAC   721 ACCGAGCTGC ANGCAGTAAA CAGGTGGCAG GGACATTGGA GCCAGANGTG GATCTCTCAC   781 CACTGCTTGC TACCAGGTAT TATCCACTGA TTCTGAAAAA TCACATTCTC CGTCATTCAG   841 NGNANANGGG GNGANATGGN NATCATAAGC CNAAAATGAG ANNACAAATG TTNACNAANT 901 CCTTANNCNN GAAANANGNT CCCNNNNANT CTTCNNNGNN NNNNNNNN //
Primer: Luc_R Region: luc2 5', mini P, spret SLGE 3' Sequence file: RDB17901_B0Afc.seq
>D06932A6_B0Af_2_Luc_R_A12_03_ABI24.ab1     1 NNNNNNNNNN NGGNNNNNNN GGCGTAGCGC TTCNTGGCTT TGTGCNGCTG CTCGCCGGCG    61 GTCCCGTCTT CGAGTGGGTA GAATGGCGCT GGGCCCTTCT TAATGTTTTT GGCATCTTCC   121 ATGGTGGCTT TACCAACAGT ACCGGATTGC CAAGCTGGAA GTCGAGCTTC CATTATATAC   181 CCTCTAGTGT CTAAGCTTAA ATGCCCATGC CAAGGGGAGC AGCCCACTCA TCCTGGTTTT   241 CCTGAACCTT GTGGTTTCAT CACAAAAGTC TTGTATCCTG GGATGCTTTA CAGTCCAGTG   301 TTCTTAGTCT CACATTACAG GACAGGTTGA GATTTGTTCT TGACCTTTTT CACTAGAGAG   361 CCTTGGAGTG CATTGAAGAT TTATTGGGAA CCTCTTTCAG GGCTAAGGAT TTTGTAAACA   421 TTTGTAGTCT CATTTTAGGC TTATGATGTC CATATCACCC CATCTCCACT GAATGACGGA   481 GAATGTGATT TTTCAGAATC AGTGGATAAT ACCTGGTAGC AAGCAGTGGT GAGAGATCCA   541 CCTCTGGCTC CAATGTCCCT GCCACCTGTT TACTGCCTGC AGCTCGGTGT GCAGGGTGGG   601 AGGCGGCTCG GCACCAGTGC TACAGACTCC CCACCTGCTT CTCTGAGGAA TGCAGCACCT   661 GTCTGCCTGC CCAAGCCTTG CCTTCCCTTC CTCTGTGGAG ATAAGAAGGA AGTAAATCCC   721 CCTGTAAAAT TCTGTACTTT CATGAGTGGT TTACTTTTGG GGGCTGANAG ACATTTTTTC   781 CTTGCCTTTA ATACAGCCCT GGCCCTCTAT AAAGGGGCCT TTCTGGNGCC NACTGTGANA   841 TACAAAGAAT CCCATGCAGT GGCTGGGANC CCGACTGACT GTTTTATCTC CACTCCTCNA   901 NGAGTGGCTC TGANNCTGGA ANGNCTTTGG CCCCNGAATG TCTTTAGTGC NNANTTTGNT   961 CACCCNNANT TGTTTGGGNG GNTNANCCTT NGNTCTTNGN CCCNNNNNCT GNGCTNACAT 1021 ANANNNNGNN NNNTCANNGG GGNNNNNNNN ANTNNNNNNN NNN //
Primer: pGL4pA_R Region: SV40 late p(A), luc2 3' Sequence file: RDB17901_B0Afd.seq
>D06932A6_B0Af_2_pGL4pA_R_B12_06_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNTNNTCNN NNTTNNCNNC NTTTGTAGAG GTTTTACTTG    61 CTTTAAAAAA CCTCCCACAC CTCCCCCTGA ACCTGAAACA TAAAATGAAT GCAATTGTTG   121 TTGTTAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT   181 TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG   241 TATCTTATCA TGTCTGCTCG AAGCGGCCGG CCGCCCCGAC TCTAGAATTA TTACACGGCG   301 ATCTTGCCGC CCTTCTTGGC CTTAATGAGA ATCTCGCGGA TCTTGCGGGC GTCCAACTTG   361 CCGGTCAGTC CTTTAGGCAC CTCGTCCACG AACACAACAC CACCGCGCAG CTTCTTGGCG   421 GTTGTAACCT GGCTGGCCAC ATAGTCCACG ATCTCCTTCT CGGTCATGGT TTTACCGTGT   481 TCCAGCACGA CGACNGCGGC GGGCAGCTCG CCGGCATCGT CGTCGGGCAG GCCGGCGACC   541 CCGGCGTCGA AGATGTTGGG GTGTTGCAGC AGGATGCTCT CCAGTTCGGC TGGGGCTACC   601 TGGTAGCCCT TGTATTTGAT CAGGCTCTTC AGCCGGTCCA CGATGAAGAA GTGCTCGTCC   661 TCGTCCCAGT AGGCGATGTC GCCGCTGTGC AGCCAGCCGT CCTTGTCGAT GAGAGCGTTT   721 GTAGCCTCGG GGTTGTTAAC GTAGCCGCTC ATGATCATGG GGCCACGGAC GCACAGCTCG   781 CCGCGCTGGT TCACACCCAG TGTCTTACCG GTGTCCAAGT CCACCACCTT AGCCTCNAAG   841 AAGGGCACCA CCTTGCCTAC TGCGCCNGGC TTGNCGTCCC CTTCNGGGGN GANCNNAANG   901 NNGCTGGTTG TTTCTGTCNN NNCGTANCCC NNNNNNANGN CTGGNNNNNN GAANCGNTNG   961 GNCNNGGNCT CNNCNACCTC CNNGNTGANC NGNGCCCCGN CNCNNGNNNN NTCNNGNNAG  1021 TTGNTTNNNN CGTNNNNNNN NANNANANNG NTNNTNNNNN NNNANNNNAA TNNNNNNGNN 1081 NNNNNCNNNN NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl