Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMRX-bsr-Myc-M-INK

M-INK, a tool for tagging mature melanosomes. Retroviral expression vector of mouse Kif1c tagged with Myc.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literatures designated by the DEPOSITOR is requested. (Ishida, M. et al. Sci. Rep. 5, 8238, 2015; Marubashi, S. et al. Cell Struct. Funct. doi: 10.1247/csf.19039, 2020)
Additional terms and conditions for distribution	Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Ishida, M. et al. Sci. Rep. 5, 8238, 2015; Marubashi, S. et al. Cell Struct. Funct. doi: 10.1247/csf.19039, 2020)。
付加的提供条件	pMRXベクターについて：利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17955_B0Cbp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F2 Region: Myc, insert 5' Sequence file: RDB17955_B0Cba.seq
>D07009A5_B0Cb_3_MMLV-pol_F2_F05_17_ABI24.ab1     1 NNNNNNGNNN NNNNNNCNNN NNGNNNGCCG GNNCNGCTTG GCATGGAGCA AAAGCTCATT    61 TCTGAAGAGG ACTTGACGCG TGATTATGAT ATTCCAACTA CTGCTAGCGA GAATTTGTAT   121 TTTCAGGGTG AGCTCGGATC TGATGATAAT GACGAGTCTG GTTTGGTCAC CTGGGCCCCA   181 CCGGAAGGGC CAGAAGCAGT AGAGGAGACA GTGCCCAACG ACCACTCACC AGCTGTCCGG   241 CCCACCTCAC CACCTCTGTC CAGCTGGGAG CGGGTGTCGA GATTGATGGA AGAGGACCCT   301 GCCTTCCGTC GCGGCCGCCT TCGCTGGCTC AAGCAGGAGC AGCTGCGGCT GCAGGGACTG   361 CAGGGCGCTG GGGGCCGGGG TGGGGGACTG CGCAGGCCTC CAGCCCGCTT TGTGCCCCCG   421 CATGATTGCA AGCTGCGCTT CCCCTTCAAA AGCAACCCTC AGCACAGGGA GTCCTGGCCA   481 GGAATGGGGA GTGGAGAGGC CCCAGCTCCT CAGCCCCCTG AAGAGGTCAC TGTCCCCCCA   541 GCACCCCCTA ACCGTAGGCC TCCAAGTCCC CGAAGACCCC ACCGTTCTCG CAGGAATTCC   601 CTAGATGGAG GGAGCCGATC TCGGGGAGGG GGTTCTACAC AGCCAGAACC CCAGCACTTA   661 CGGCCCCAAA AGCACAACGG TTATCCCCAA CAGCCCCAGC CCAGCCCAGC CCAGCGGCCA   721 GGGCCCCGCT ACCCACCATA CACTACCCCT CCGCGAATGC GACGGCAGCG CTCAGCCCCT   781 GNNCTCAAAG AGAGTGGGGC AGCTGTGTNN NNCACTAGTG AATTCGCGGC CGCGGTAACA   841 ATTGTTAACT AACTTAAGCT AGCAACGGTT TCCCTCTANC GGGNTCAATT NCGCCCCCCC   901 CCCCCNNNAC GTTACTGGNC NAANCCGCTT NNNANNAANG NNNGGNGTGC GTTTGTCTAT 961 NTGNTNNTTT TCCACCNTAN NGNNGNNNTT NNNNNNNGNN NNNNNNNN //
Primer: IRES-R Region: IRES, insert 3' Sequence file: RDB17955_B0Cbb.seq
>D07009A5_B0Cb_3_IRES-R_F04_16_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNT TNGGNNNNNG GGGCGGAATT GATCCCGCTA    61 GAGGGAAACC GTTGCTAGCT TAAGTTAGTT AACAATTGTT ACCGCGGCCG CGAATTCACT   121 AGTGATTTCA CACAGCTGCC CCACTCTCTT TGAGGTCAGG GGCTGAGCGC TGCCGTCGCA   181 TTCGCGGAGG GGTAGTGTAT GGTGGGTAGC GGGGCCCTGG CCGCTGGGCT GGGCTGGGCT   241 GGGGCTGTTG GGGATAACCG TTGTGCTTTT GGGGCCGTAA GTGCTGGGGT TCTGGCTGTG   301 TAGAACCCCC TCCCCGAGAT CGGCTCCCTC CATCTAGGGA ATTCCTGCGA GAACGGTGGG   361 GTCTTCGGGG ACTTGGAGGC CTACGGTTAG GGGGTGCTGG GGGGACAGTG ACCTCTTCAG   421 GGGGCTGAGG AGCTGGGGCC TCTCCACTCC CCATTCCTGG CCAGGACTCC CTGTGCTGAG   481 GGTTGCTTTT GAAGGGGAAG CGCAGCTTGC AATCATGCGG GGGCACAAAG CGGGCTGGAG   541 GCCTGCGCAG TCCCCCACCC CGGCCCCCAG CGCCCTGCAG TCCCTGCAGC CGCAGCTGCT   601 CCTGCTTGAG CCAGCGAAGG CGGCCGCGAC GGAAGGCAGG GTCCTCTTCC ATCAATCTCG   661 ACACCCGCTC CCAGCTGGAC AGAGGTGGTG AGGTGGGCCG GACAGCTGGT GAGTGGTCGT   721 TGGGCACTGT CTCCTCTACT GCTTCTGGCC CTTCCGGTGG GGCCCAGGTG ANCAAACCAN   781 ACTCGTCATT ATCATCAGAT CCGANCTCAC CCTGAAAATA CNAATTCTCN NNNNCAGTAG   841 TTGGAATATC ATAATCACGC NNCAAGNNCN TCTTCAGAAA TGANNTTTTG NTCCATGCCN   901 AGCNNATNCG GCANTCTANA NGNANGGTCC ACCCCCGGGG TCNGNCNGCC TNNNNGNGGG   961 CGGCNTGNAT CCNANCTGCN ANGCCGNCTA NTTTGNNGGN NNGNNGGGGG GNNGNNNNNN 1021 NNGANNNNGN NNNNNNNNN //
Primer: IRES-F Region: IRES, bsr Sequence file: RDB17955_B0Cbc.seq
>D07009A5_B0Cb_3_IRES-F_G04_19_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNAAA AACGTCTAGG CCCCCCGAAC    61 CNCGGGGACG TGGTTTTCCT TTGAAAAACA CGATAATACC ATGGTCATGA AAACATTTAA   121 CATTTCTCAA CAAGATCTAG AATTAGTAGA AGTAGCGACA GAGAAGATTA CAATGCTTTA   181 TGAGGATAAT AAACATCATG TGGGAGCGGC AATTCGTACG AAAACAGGAG AAATCATTTC   241 GGCAGTACAT ATTGAAGCGT ATATAGGACG AGTAACTGTT TGTGCAGAAG CCATTGCGAT   301 TGGTAGTGCA GTTTCGAATG GACAAAAGGA TTTTGACACG ATTGTAGCTG TTAGACACCC   361 TTATTCTGAC GAAGTAGATA GAAGTATTCG AGTGGTAAGT CCTTGTGGTA TGTGTAGGGA   421 GTTGATTTCA GACTATGCAC CAGATTGTTT TGTGTTAATA GAAATGAATG GCAAGTTAGT   481 CAAAACTACG ATTGAAGAAC TCATTCCACT CAAATATACC CGAAATTAAA AGTCGACGAT   541 AAAATAAAAG ATTTTATTTA GTCTCCAGAA AAAGGGGGGA ATGAAAGACC CCACCTGTAG   601 GTTTGGCAAG CTAGCTTAAG TAACGCCATT TTGCAAGGCA TGGAAAAATA CATAACTGAG   661 AATAGAGAAG TTCAGATCAA GGTCAGGAAC NNNTGGAACA GCTGAATATG GGCCAAACAG   721 GATATCTGTG GTAAGCAGTT CCTGCCCCGG CTCANGGCCA AGANCNGATG GAACAGCTGA   781 NTATGGGCNA AACAGGATAT CTGTGGTAAG CAGTTCCTGC CCCGGCTCNN NGCCAAGAAC   841 ANANGGTCCC CAGATGCGGT CCAGCCCTCA NCAGNTNCTN NANNNNCNTC NNATGTTTNC   901 NNGGTGCCCC NANGGANCTG AAATGANCCT GTGCCNTATT TGAACTAACN NATCAGTTCG   961 CTTNTNGCTN CTGNTCNNGC GCNTCNGNTC CCCGAGCTNN NNNAAANANC NNNNANNCCN 1021 NNNNNNNNN //
Primer: CMV enh_F1 Region: 5' LTR, psi Sequence file: RDB17955_B0Cbd.seq
>D07009A5_B0Cb_3_CMV enh_F1_H04_22_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNGNNNNGNN NNTGANCTTA TGGGACTTTN NNNNNNGGCA    61 GTACATCTAC GTATTAGTCA TCGCTATTAC CATGCTAGAG AACCATCAGA TGTTTCCAGG   121 GTGCCCCAAG GACCTGAAAT GACCCTGTGC CTTATTTGAA CTAACCAATC AGTTCGCTTC   181 TCGCTTCTGT TCGCGCGCTT CTGCTCCCCG AGCTCAATAA AAGAGCCCAC AACCCCTCAC   241 TCGGCGCGCC AGTCCTCCGA TTGACTGAGT CGCCCGGGTA CCCGTGTATC CAATAAACCC   301 TCTTGCAGTT GCATCCGACT TGTGGTCTCG CTGTTCCTTG GGAGGGTCTC CTCTGAGTGA   361 TTGACTACCC GTCAGCGGGG GTCTTTCATT TGGGGGCTCG TCCGGGATCG GGAGACCCCT   421 GCCCAGGGAC CACCGACCCA CCACCGGGAG GTAAGCTGGC CAGCAACTTA TCTGTGTCTG   481 TCCGATTGTC TAGTGTCTAT GACTGATTTT ATGCGCCTGC GTCGGTACTA GTTAGCTAAC   541 TAGCTCTGTA TCTGGCGGAC CCGTGGTGGA ACTGACGAGT TCGGAACACC CGGCCGCAAC   601 CCTGGGAGAC GTCCCAGGGA CTTCGGGGGC CGTTTTTGTG GCCCGACCTG AGTCCAAAAA   661 TCCCGATCGT TTTGGACTCT TTGGTGCACC CCCCTTAGAG GAGGGATATG TGGTTCTGGT   721 AGGAGACGAG AACCTAAAAC AGTTCCCGCC TCCGTCTGAA TTTTTGCTTT CGGTTTGGGA   781 NCGAAGCCGC GCCGCGCGTC TTGTCTGCTG CANCATCGTT CTGTGTTGTC TCTGTCTGAC   841 TGTGTTTCTG TATTTGTCTG AAAATATGGG NCCGGGCCAN ANTGNNNCCN CTNCCTTAAG   901 TTTGANCTTN NGTCANTGGA AANANGNCNA NCGGNTCGNT NNCNACCNGN CNGTANNNGT 961 CAAGANNANN NGTTNGGGNT ANCNTNNGNT CTNNNNAANN NNNNNNNN //
Primer: bsr-C_F Region: 3' LTR Sequence file: RDB17955_B0Cbe.seq
>D07009A5_B0Cb_3_bsr-C_F_A05_02_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN AANNNNTTCC NCTNNNATAT ACCCGAAATT    61 NNNANTCGAC GATAAAATAN NNNNNTTTAT TTANTCTCCN GAAAAAGGGG GGAATGAAAG   121 ACCCCACCTG TAGGTTTGGC AAGCTAGCTT AAGTAACGCC NTTTTGCAAG GCATGGAAAA   181 ATACATAACT GAGAATAGAG AAGTTCAGAT CAAGGTCAGG AACAGATGGA ACAGCTGAAT   241 ATGGGCCAAA CAGGATATCT GTGGTAAGCA GTTCCTGCCC CGGCTCAGGG CCAAGAACAG   301 ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT   361 CAGGGCCAAG AACAGATGGT CCCCAGATGC GGTCCAGCCC TCAGCAGTTT CTAGAGAACC   421 ATCAGATGTT TCCAGGGTGC CCCAAGGACC TGAAATGACC CTGTGCCTTA TTTGAACTAA   481 CCAATCAGTT CGCTTCTCGC TTCTGTTCGC GCGCTTCTGC TCCCCGAGCT CAATAAAAGA   541 GCCCACAACC CCTCACTCGG GGCGCCAGTC CTCCGATTGA CTGAGTCGCC CGGGTACCCG   601 TGTATCCNAT AAACCCTCTT GCAGTTGCAT CCGACTTGTG GTCTCGCTGT TCCTTGGGAG   661 GGTCTCCTCT GAGTGATTGA CTACCCGTCA GCGGGGGTCT TTCNNNTGNN NNATGTATCA   721 AAATTANNTT GGNTTTTTTT CTNNANGTAT TTACATTAAA ATGGCCATAG TTGCATTAAT   781 GAANTCGGCC NAACGCGCGG GGNANANGCG GTTTGCNTAT TGGGCGCTCT TCCGCTTCCN   841 CGCTCACNGA CTCGCTGCGC TCGGNCGTNN GNNTGCNGCN ANCGNNATCA NCTCACTCNA   901 AGGNNGTAAT ACNGNTATCC NCAGANTCNN GGNANNNCGC NNNNNANNNN NNNTGANCNA   961 NNNGNCANNN AANGGNNNNG NANNNNNAAA ANNNCNGCGT TGCTNGNNNN TTTTTNNNNN 1021 GNNNNNNNNN N //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl