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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMRX-bsr-mCherry-M-INK

M-INK, a tool for tagging mature melanosomes. Retroviral expression vector of mouse Kif1c tagged with mCherry.

Catalog number RDB17956
Resource name pMRX-bsr-mCherry-M-INK
Clone info. M-INK, a tool for tagging mature melanosomes. Retroviral expression vector of mouse kinesin family member 1C (Kif1c) tagged with mCherry at N-terminus.
Comment Visullization of melanosomes. Expression was confirmed by the depositor with immunoblotting.
Vector backbone pMRX-bsr-mCherry (plasmid)
Selectable markers Ampicillin (E. coli), Blasticidin (mammalian cells).
Gene/insert name mouse Kif1c cDNA
Depositor|Developer Fukuda, Mitsunori |
Other clones in our bank

External Database
mouse Kif1c

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literatures designated by the DEPOSITOR is requested. (Ishida, M. et al. Sci. Rep. 5, 8238, 2015; Marubashi, S. et al. Cell Struct. Funct. doi: 10.1247/csf.19039, 2020)
          Additional terms and conditions for distribution Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks Remember that you will be working with samples containing infectious virus.
          This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Ishida, M. et al. Sci. Rep. 5, 8238, 2015; Marubashi, S. et al. Cell Struct. Funct. doi: 10.1247/csf.19039, 2020)。
          付加的提供条件 pMRXベクターについて:利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
          Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB17956 pMRX-bsr-mCherry-M-INK DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Marubashi, S., Rab7B/42 Is Functionally Involved in Protein Degradation on Melanosomes in Keratinocytes. Cell Struct. Funct. 45 (1): 45-55 (2020). PMID 32037382.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Visualization of Organelles (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB17956_B0Cbp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: mCherry-C_F
          Region: mCherry 3', insert 5'
          Sequence file: RDB17956_B0Cba.seq check
          >D07009A6_B0Cb_2_mCherry-C_F_E01_05_ABI08.ab1
              1 NNNNNNNNNN NNNNNNGCTG CNNGGCGCCT ACNACGTCNA CATCAAGTTG GACATCACCT
             61 CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAACG CGCCGAGGGC CGCCACTCCA
            121 CCGGCGGCAT GGACGAGCTG TACAAGTCCG GACTCGGATC TGATGATAAT GACGAGTCTG
            181 GTTTGGTCAC CTGGGCCCCA CCGGAAGGGC CAGAAGCAGT AGAGGAGACA GTGCCCAACG
            241 ACCACTCACC AGCTGTCCGG CCCACCTCAC CACCTCTGTC CAGCTGGGAG CGGGTGTCGA
            301 GATTGATGGA AGAGGACCCT GCCTTCCGTC GCGGCCGCCT TCGCTGGCTC AAGCAGGAGC
            361 AGCTGCGGCT GCAGGGACTG CAGGGCGCTG GGGGCCGGGG TGGGGGACTG CGCAGGCCTC
            421 CAGCCCGCTT TGTGCCCCCG CATGATTGCA AGCTGCGCTT CCCCTTCAAA AGCAACCCTC
            481 AGCACAGGGA GTCCTGGCCA GGAATGGGGA GTGGAGAGGC CCCAGCTCCT CAGCCCCCTG
            541 AAGAGGTCAC TGTCCCCCCA GCACCCCCTA ACCGTAGGCC TCCAAGTCCC CGAAGACCCC
            601 ACCGTTCTCG CAGGAATTCC CTAGATGGAG GGAGCCGATC TCGGGGAGGG GGTTCTACAC
            661 AGCCAGAACC CCAGCACTTA CGGCCCCAAA AGCACAACGG TTATCCCCNA CAGCCCCAGC
            721 CCAGCCCAGC CCAGCGGCCA GGGCCCCGCT ACCCACCATA CACTACCCCT CCGCGAATGC
            781 GACGGCAGCG CTCAGCCCCT GACCTCNAAG AGAGTGGGGC AGCTGTGTGA AATCACTAGT
            841 GAANTCGCGG NCGCGGTAAC NANNGNNAAC NNACTTAAGC TAGCNACGGT TTCCNNCTAG
            901 CGGGNTCNNT TNCGCCCCCC CNNCCNNNAC GTTNCNGGNN NGANNCCGCN TNNNNNNANN
          961 NNNGNNNNNN GTTTGNNNNN NTGNNNNTTT NCNNNNNNNN NN
          //
          Primer: IRES-R
          Region: insert 3'
          Sequence file: RDB17956_B0Cbb.seq check
          >D07009A6_B0Cb_2_IRES-R_C05_08_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNACG TNNNGGGGGG GGGGCGGNNN NTGANNCCCG
             61 CTAGAGGGAA ACCGTTGCTA GCTTAAGTTA GTTAACAATT GTTACCGCGG CCGCGAATTC
            121 ACTAGTGATT TCACACAGCT GCCCCACTCT CTTTGAGGTC AGGGGCTGAG CGCTGCCGTC
            181 GCATTCGCGG AGGGGTAGTG TATGGTGGGT AGCGGGGCCC TGGCCGCTGG GCTGGGCTGG
            241 GCTGGGGCTG TTGGGGATAA CCGTTGTGCT TTTGGGGCCG TAAGTGCTGG GGTTCTGGCT
            301 GTGTAGAACC CCCTCCCCGA GATCGGCTCC CTCCATCTAG GGAATTCCTG CGAGAACGGT
            361 GGGGTCTTCG GGGACTTGGA GGCCTACGGT TAGGGGGTGC TGGGGGGACA GTGACCTCTT
            421 CAGGGGGCTG AGGAGCTGGG GCCTCTCCAC TCCCCATTCC TGGCCAGGAC TCCCTGTGCT
            481 GAGGGTTGCT TTTGAAGGGG AAGCGCAGCT TGCAATCATG CGGGGGCACA AAGCGGGCTG
            541 GAGGCCTGCG CAGTCCCCCA CCCCGGCCCC CAGCGCCCTG CAGTCCCTGC AGCCGCAGCT
            601 GCTCCTGCTT GAGCCAGCGA AGGCGGCCGC GACGGAAGGC AGGGTCCTCT TCCATCAATC
            661 TCGACACCCG CTCCCAGCTG GACAGAGGTG GTGAGGTGGG CCGGACAGCT GGTGAGTGGT
            721 CGTTGGGCAC TGTCTCCTCT ACTGCTTCTG GCCCTTCCGG TGGGGGNCCA GGTGACCAAA
            781 CCAGACTCGT CATTATCATC NNATNCNAGT CNNGANTTGN ACAGCTCNTC CATGCCGCCG
            841 GNGGANTGGC GGNNNCTCGG CGCGTNCGNN CTGNNCCNCG ATGGTGTANN NNCTNGNTNG
            901 NNGGNNGTGA TGTCCAANTT GATGTTGANG TNGTANGNGN CGGGCNNCNN CNNNGGNNTT
            961 CNNGNNNTNN NNGGNGGNCT TGANCNNCNN CNNNNNANNN GNCNGNNNTN NNTNNAGCTT
          1021 CANCCNNNNG NNTNNATCTC NNNNNNTNNN NNGNNNNCNN NNNNNNNN
          //
          Primer: MMLV-pol_F
          Region: mCherry 5'
          Sequence file: RDB17956_B0Cbc.seq check
          >D07009A6_B0Cb_2_MMLV-pol_F_B05_05_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNGNNTCNNN AGCTTGGNNA CACGCCGCCN NCGTGAAGGC
             61 TGCCGACCCC GGGGGTGGAC CATCCTCTAG ACTGCCGGAT CCGCCGCCAC CATGGTGAGC
            121 AAGGGCGAGG AGGATAACAT GGCCATCATC AAGGAGTTCA TGCGCTTCAA GGTGCACATG
            181 GAGGGCTCCG TGAACGGCCA CGAGTTCGAG ATCGAGGGCG AGGGCGAGGG CCGCCCCTAC
            241 GAGGGCACCC AGACCGCCAA GCTGAAGGTG ACCAAGGGTG GCCCCCTGCC CTTCGCCTGG
            301 GACATCCTGT CCCCTCAGTT CATGTACGGC TCCAAGGCCT ACGTGAAGCA CCCCGCCGAC
            361 ATCCCCGACT ACTTGAAGCT GTCCTTCCCC GAGGGCTTCA AGTGGGAGCG CGTGATGAAC
            421 TTCGAGGACG GCGGCGTGGT GACCGTGACC CAGGACTCCT CCCTGCAGGA CGGCGAGTTC
            481 ATCTACAAGG TGAAGCTGCG CGGCACCAAC TTCCCCTCCG ACGGCCCCGT AATGCAGAAG
            541 AAGACCATGG GCTGGGAGGC CTCCTCCGAG CGGATGTACC CCGAGGACGG CGCCCTGAAG
            601 GGCGAGATCA AGCAGAGGCT GAAGCTGAAG GACGGCGGCC ACTACGACGC TGAGGTCAAG
            661 ACCACCTACA AGGCCAAGAA GCCCGTGCAG CTGCCCGGCG CCTACAACGT CAACATCAAG
            721 TTGGACNTCA CCTCCCACAA CGAGGACTAC ACCATCGTGG AACAGTACGA ACGCGCCGAG
            781 GGCCGCCACT CCACCGGCGG CATGGACGAN CTGTACAAGT CCGGACTCNG ATCTGATGAT
            841 AATGACGAGT CTGGTTTGNT CACCTGGGCC CCNCCNGAAG GGCCAGAANC AGTANAGGAG
            901 ANNGTGCCCN NCGANCACTC ANCAGCTGTC CNGCCCANCT CANCNNCTCT GTCCNGCTGG
            961 GANNNNNGNC NANANTGNNG GAANANNANN NNNNNTNNNN NNNNNGNNNN NCNNNNNNNN
          1021 NN
          //
          Primer: IRES-F
          Region: IRES, bsr
          Sequence file: RDB17956_B0Cbd.seq check
          >D07009A6_B0Cb_2_IRES-F_F01_06_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNT NNNNTCGANG TTAANNNNNN TCTAGGCCCC CCGAACCNCG
             61 GGGNCGTGGT TTTCCTTTGA AAAACACGAT AATACCATGG TCATGAAAAC ATTTAACATT
            121 TCTCAACAAG ATCTAGAATT AGTAGAAGTA GCGACAGAGA AGATTACAAT GCTTTATGAG
            181 GATAATAAAC ATCATGTGGG AGCGGCAATT CGTACGAAAA CAGGAGAAAT CATTTCGGCA
            241 GTACATATTG AAGCGTATAT AGGACGAGTA ACTGTTTGTG CAGAAGCCAT TGCGATTGGT
            301 AGTGCAGTTT CGAATGGACA AAAGGATTTT GACACGATTG TAGCTGTTAG ACACCCTTAT
            361 TCTGACGAAG TAGATAGAAG TATTCGAGTG GTAAGTCCTT GTGGTATGTG TAGGGAGTTG
            421 ATTTCAGACT ATGCACCAGA TTGTTTTGTG TTAATAGAAA TGAATGGCAA GTTAGTCAAA
            481 ACTACGATTG AAGAACTCAT TCCACTCAAA TATACCCGAA ATTAAAAGTC GACGATAAAA
            541 TAAAAGATTT TATTTAGTCT CCAGAAAAAG GGGGGAATGA AAGACCCCAC CTGTAGGTTT
            601 GGCAAGCTAG CTTAAGTAAC GCCATTTTGC AAGGCATGGA AAAATACATA ACTGAGAATA
            661 GAGAAGTTCA GATCAAGGTC AGGAACAGAT GGAACAGCTG AATATGGGCC AAACAGGATA
            721 TCTGTGGTAA GCAGTTCCTG CNCCGGCTCA NGGCCAAGAN CAGATGGAAC AGCTGAATAT
            781 GGGNCCAAAC AGGATATCTG TGGTAAGCAG TTCCTGCCCC GGCTCNNNNC AAGAACAGAT
            841 GGNCCCCANA NGNNNNCCAN CCNNNNNCAN TTTCTAGAGA NNNNTCANAN GNTTNCNNGG
            901 NGNCCCANNG ANNNNNANNG ANCNNNNNNC NTNATTTGAN NNNNNNANCN NNNCNCTNNN
          961 CNNNNCNNNN NNNNNN
          //
          Primer: CMV enh_F1
          Region: 5' LTR, psi
          Sequence file: RDB17956_B0Cbe.seq check
          >D07009A6_B0Cb_2_CMV enh_F1_E05_14_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNGCNN NNNNNNTGAC CTTATGGGAN TTTCCTACTT
             61 GGCAGTACAT CTACGTATTA GTCNTCNNTA TTACCATGCT AGAGAACCAT CAGATGTTTC
            121 CAGGGTGCCC CAAGGACCTG AAATGACCCT GTGCCTTATT TGAACTAACC AATCAGTTCG
            181 CTTCTCGCTT CTGTTCGCGC GCTTCTGCTC CCCGAGCTCA ATAAAAGAGC CCACAACCCC
            241 TCACTCGGCG CGCCAGTCCT CCGATTGACT GAGTCGCCCG GGTACCCGTG TATCCAATAA
            301 ACCCTCTTGC AGTTGCATCC GACTTGTGGT CTCGCTGTTC CTTGGGAGGG TCTCCTCTGA
            361 GTGATTGACT ACCCGTCAGC GGGGGTCTTT CATTTGGGGG CTCGTCCGGG ATCGGGAGAC
            421 CCCTGCCCAG GGACCACCGA CCCACCACCG GGAGGTAAGC TGGCCAGCAA CTTATCTGTG
            481 TCTGTCCGAT TGTCTAGTGT CTATGACTGA TTTTATGCGC CTGCGTCGGT ACTAGTTAGC
            541 TAACTAGCTC TGTATCTGGC GGACCCGTGG TGGAACTGAC GAGTTCGGAA CACCCGGCCG
            601 CAACCCTGGG AGACGTCCCA GGGACTTCGG GGGCCGTTTT TGTGGCCCGA CCTGAGTCCA
            661 AAAATCCCGA TCGTTTTGGA CTCTTTGGTG CACCCCCCTT ANAGGAGGGA TATGTGGTTC
            721 TGGTANGAGA CNANAACCTA AAACAGTTCC CGCCTCCGTC TGAATTTTTG CTTTCGGTTT
            781 GGGANCNANN NCGCGCCGCG CGTCTTGTCT GCTGCAGCAT CGTTCTGTGN TGTCTCTGTC
            841 TGACTGTGTT TCTGTANTTG TCTGAAAAAT ATGGGCCCGG NNNANACTGN NNNCACNCCN
            901 NTANNNTTGA CCTTANGTCA NTGGNNAANA NGNNNNNCGN NTCGCTCACN NCNNNNNCGG
            961 TNNANGTNNA NNAANANANN NNNGGNNNNC NTNNTGNTCT GNNNAANNNG NCCNANNNTT
          1021 TNAACNNNNN GNNNNGNNNN NNNNNN
          //
          Primer: bsr-C_F
          Region: 3' LTR
          Sequence file: RDB17956_B0Cbf.seq check
          >D07009A6_B0Cb_2_bsr-C_F_F05_17_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGNN NNNNNNNNNG AATGGCAAGT TNGTCAAAAC
             61 TACGATTGAA GAACTCATTC CACTCAAATA TACCCGAAAT TAAAAGTCGA CGATAAAATA
            121 AAAGATTTTA TTTAGTCTCC AGAAAAAGGG GGGAATGAAA GACCCCACCT GTAGGTTTGG
            181 CAAGCTAGCT TAAGTAACGC CATTTTGCAA GGCATGGAAA AATACATAAC TGAGAATAGA
            241 GAAGTTCAGA TCAAGGTCAG GAACAGATGG AACAGCTGAA TATGGGCCAA ACAGGATATC
            301 TGTGGTAAGC AGTTCCTGCC CCGGCTCAGG GCCAAGAACA GATGGAACAG CTGAATATGG
            361 GCCAAACAGG ATATCTGTGG TAAGCAGTTC CTGCCCCGGC TCAGGGCCAA GAACAGATGG
            421 TCCCCAGATG CGGTCCAGCC CTCAGCAGTT TCTAGAGAAC CATCAGATGT TTCCAGGGTG
            481 CCCCAAGGAC CTGAAATGAC CCTGTGCCTT ATTTGAACTA ACCAATCAGT TCGCTTCTCG
            541 CTTCTGTTCG CGCGCTTCTG CTCCCCGAGC TCAATAAAAG AGCCCACAAC CCCTCACTCG
            601 GGGCGCCAGT CCTCCGATTG ACTGAGTCGC CCGGGTACCC GTGTATCCAA TAAACCCTCT
            661 TGCAGTTGCA TCCGACTTGT GGTCTCGCTG TTCCTTGGGA GGGTCTCCTC TGAGTGATTG
            721 ACTACCCGTC AGCGGGGGTC TTTCACATGC AGCATGTATC AAAATTAATT TGGTTTTTTT
            781 TCTNAAGTAT TTACNTTAAA TGGCCATAGT TGCNTTAATG ANTCGGCNNA NCGCGCGGGG
            841 AGAGGCGGTT TGCGTATTGG GGCGCTNTTC CGNTTCCTCG CTCACTGACT CNNNGCGCTC
            901 GGNCGTTCGG CTGCGGNGAN CGGTATCANC TCNCTCNAAN GNGGTAATAC NNNTNTNNNN
            961 NGANNCNGGG ATNACNCNNN NAANANNNTG TGANCAAANN NNNNNNAAAN GNNNNNNNNN
          1021 N
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Marubashi, S., Rab7B/42 Is Functionally Involved in Protein Degradation on Melanosomes in Keratinocytes. Cell Struct. Funct. 45 (1): 45-55 (2020). PMID 32037382.
          reference Ishida, M., Rab1A regulates anterograde melanosome transport by recruiting kinesin-1 to melanosomes through interaction with SKIP. Sci. Rep. 5: 8238 (2015). PMID 25649263.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl