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EGFP-ZBTB24 mutZF8 (mouse)

Expression vector of mouse Zbtb24 mutZF8 (C494Y).

Catalog number RDB18182
Resource name EGFP-ZBTB24 mutZF8 (mouse)
Clone info. Expression vector of mouse zinc finger and BTB domain containing 24 (Zbtb24) mutZF8 (C494Y), fused with N-terminal EGFP, CMV promoter.
Comment Expression was confirmed by the depositor with western blotting and fluorescence microscope.
Mutagenesis: forward primer (5'- ATGGCTTACAGTTCGCTCGC -3'), reverse primer (5'- ACTCGGGGCAGGAGAAGG -3').
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cells)
Gene/insert name mouse Zbtb24 cDNA
Depositor|Developer Unoki, Motoko |
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External Database
mouse Zbtb24

          Reference sequence
            
           
          Sequence (partial) RDB18182pka01.seq provided by the depositor

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Aktar, S. et al., Genes Cells. 24 (11), 746-755, 2019)
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Aktar, S. et al., Genes Cells. 24 (11), 746-755, 2019)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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          RDB18182 EGFP-ZBTB24 mutZF8 (mouse) Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Original reference

          original Aktar, S., Identification of ZBTB24 protein domains and motifs for heterochromatin localization and transcriptional activation. Genes Cells 24 (11): 746-755 (2019). PMID 31561277.

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Aktar, S., Identification of ZBTB24 protein domains and motifs for heterochromatin localization and transcriptional activation. Genes Cells 24 (11): 746-755 (2019). PMID 31561277.

          2023.04.06

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