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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCI-neo-rPCFT

Expression vector of rat PCFT.

Catalog number RDB18231
Resource name pCI-neo-rPCFT
Clone info. Expression vector of rat proton-coupled folate transporter/heme carrier protein 1 (PCFT).
Comment A reference nucleotide sequence for Rat PCFT is NM_001013969.1.
Vector backbone pCl-neo (plasmid)
Size of vector backbone 5.5 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell).
Gene/insert name rat Slc46a1 cDNA
Depositor|Developer Yuasa, Hiroaki |
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External Database
rat Slc46a1

          Reference sequence
            
           
          Sequence (full) RDB18231zzk01.seq provided by the depositor

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested.(Inoue K. et al. Am. J. Physiol. 294, G660-G668, 2008) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. This material is provided “as is” and without any warranty express or implied, including any warranty of merchantability or fitness for a particular purpose or that the use of the material will not infringe or violate any patent or other intellectual property rights of any third party or that the material will not pose a health or safety risk. The DEPOSITOR shall not be liable for any use of the material by the RECIPIENT, or for any loss, claim, damage, or liability of any kind or nature, which may arise from or in connection with this agreement or from the use, handling, or storage of the material.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Inoue K. et al. Am. J. Physiol. 294, G660-G668, 2008)。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。当該利用者によるリソースの利用が第三者の産業財産権をはじめとする一切の知的財産権を侵害しない旨の保証をせず、かつ商品性又は特定目的への適合性の保証をしない。また、当該利用者による本件成果有体物の使用、保有によって発生した如何なる結果、損害についても一切その責任を負担せず、かつ、如何なる損害賠償義務(直接、間接損害を問わない。)も負わない。
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          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB18231 pCI-neo-rPCFT DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Inoue, K., Functional characterization of PCFT/HCP1 as the molecular entity of the carrier-mediated intestinal folate transport system in the rat model. Am. J. Physiol. Gastrointest. Liver Physiol. 294(3): G660-668 (2008). PMID 18174275.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18231_B0Fup1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: chimeric intron 3-prime
          Region: T7 pro, insert 5'
          Sequence file: RDB18231_B0Fua.seq check
          >D07213A2_B0Fu_2_chimeric intron3-prime_D06_12_ABI24.ab1
              1 NNNNNNNNNN NCNNNNNNNN NNNCNGCTCT TAAGGCTAGA GTACTTAATA CGACTCACTA
             61 TAGGCTAGCC TCGAGAATTC CGGAATTTCC TCGAGGTCGA CAGATCTGAT GTGTCAGCCC
            121 CTCGGTGTTC GCGCATGGAG GGGCGCGTGA GCCCCGTGGG CTCGTCCCAC CGTCTCCTCA
            181 CCGCCGCCGT GCTGTTCCGC GGCCCCGTGG AGCCCCTCGT TTTCCTAGCC AACTTTGCGC
            241 TGGTTCTACA GGGACCGCTC ACCACACAGT ACATTTGGCA CCGCATCAGC ACCGAGCTCG
            301 GATACAATGG CACCCGCCAC CGGGAGAACT GTGGCAACCA GAGCGCGGAT CCCGTCCTGA
            361 AGGAAGTGGA GACCCTGACA TCCCACTGGA CCCTCTACAT GAACGTGGGA GGCTTCCTGG
            421 TGGGACTCTT CTGGTCCACC CTCCTGGGAG CCTGGAGTGA CCGCGTGGGT CGCCGCCCAT
            481 TGCTGGTGCT GGCCTCTCTT GGTCTGCTGC TCCAGGCCGT GGTGTCCATC TTTGTGGTGC
            541 AACTACAACT GCACATCGGG TTCTTCGTGC TCGGCCGAGC CCTTTGTGCC CTTCTGGGAG
            601 NTTTCAACGG CCTTCTTGCT GCTAGCTTTG CCTCTGTGGC TGATGTTAGC TCTAATCACA
            661 GTCGCACCTT CCGGATGGCT CTGCTGGAAN CATGCATTGG TGTGGCCGGG ACCCTGGCAA
            721 GCCTTCTTGG GGGTCACTGG CTCCNGGCCC AGGGTTATGC CNACCCTTTC TGGCTGGCTT
            781 TGGCTGTGCT GATCGTTATG ANTCTATATG CAGCATTCTG TTTTGGTGAN ACAGNNNANG
            841 ANCNNANNNN CNCNANGNTT TTTCACGCNN NNNCNTCACC NATCCNNNNN NCANCNNNNN
          901 NNNNN
          //
          Primer: pAxCALNLF1
          Region: insert 3'
          Sequence file: RDB18231_B0Fub.seq check
          >D07213A2_B0Fu_2_pAxCALNLF1_E06_15_ABI24.ab1
              1 NNNNNNNNNN NNNANNNNNN CTGCTCGNNN CNTTAACCCT CACTAAAGGG AAGCGGCCGC
             61 CCGGGTCGAC AGATCTGATG CNNNNNNACT GGGCTTTAGG TATGTAGTCG GTGTGGAGCC
            121 ACTGCTTCTC ATGGCTTCTG GCAGCTCAGG AGTTCTGAGG AAACTGCTGG AACTCTGGGT
            181 GTGGATTAAC CTTCTCCAGT ACCCCAATCA GAATGGCCGG GATAAAGAGG AGGCCAGCTC
            241 CCAGGAGGAA GGGGAAGCCC TTCATGAAGT TCAGAGTGGC CGGGTAGAGT GAGTTGAAGA
            301 TGCCGGAGGC CATCAACATG GCCAAGCTAT TCACACAGGC CACAGCCGAA AAGAGAGCAC
            361 CCTGCTCCGA CTCGCTCACT AGCTTGGAGA GCTTGGCCCG GATCACAGGC GTAGTGACCA
            421 ATGACAGGAA GAGTAATCCG TACCCTGTGA ACATGAGGGG TGTGATGGTT GCGAACGCAA
            481 AGACCACCAT TCCCAGGATG TTGAAGGCCA GGCCAATCTC AGCCACCCAG GTGTCGGCCA
            541 GGCAGAACTG CAGAAGTCTC AGGCCCAGCA GGCTGGTGAG GTAGGGTAAG TGCTGGGCTG
            601 CTGANCCATA GCCAATCAGC TTGGAGTCCC AGCAGANGGG TGTGCTTAGC TCATAGAGCG
            661 TCANGNTGTC TTGAGCCCCA AAGTGCACAG TGACCACCAC GAATATGGNC AATGANTACA
            721 GGGCTAGATG CATCCTGNAC TTCTCTGGGG CTGGGANCAC ATACAGCTGG NCAATGGNTC
            781 GGNGATGGCG GANCGNGAAA AGCCNCNNNG NNTTTGGCTC CTTCACTGNC TCACCAAAAC
            841 ANANNGCTNN NNATANNNNC ATNNCGATCA NCACNNCCNA ANNNNGCCNN AANNGNNNNN
          901 GNNTANNNNN NGNNNNNNNN NN
          //
          Primer: SV40pro_ori_F
          Region: SV40 pro_ori, NeoR_KanR
          Sequence file: RDB18231_B0Fuc.seq check
          >D07213A2_B0Fu_2_SV40pro_ori_F_F06_18_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNGNN GCCTCNGCCT CTGNNCTATT CNNGAAGTAG TGAGGAGGCT
             61 TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TTGATTCTTC TGACACAACA GTCTCGAACT
            121 TAAGGCTAGA GCCACCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG
            181 GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC
            241 CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG
            301 TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT
            361 TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG
            421 CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT
            481 CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA
            541 CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA
            601 GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA
            661 GGCGCGCATG CCCGACGGCG ANGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA
            721 TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC
            781 NGNNCGCTAT CANGNCATAN CGTTGGCNAC CCGNNATATT GCTGAANANC TNGGCGGCGA
            841 ANGGGNCNGA NCGCTTCCNC NNGCTTTACN NNATCGCCGC NCCCNATNCN NANCGNATCN
          901 NCTTCNATCN NCTTCNNNNN NNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Inoue, K., Functional characterization of PCFT/HCP1 as the molecular entity of the carrier-mediated intestinal folate transport system in the rat model. Am. J. Physiol. Gastrointest. Liver Physiol. 294(3): G660-668 (2008). PMID 18174275.

          2022.08.12

          GNP_filter3_RDBDEP_html_220812.pl