Please review the QC test results indicated by icon below as well as clone information before placing your order.

pAY7

TOL2 expression vector of OsTIR1(WT) and mAID-EGFP-NLS marker.

Sequence (full)	RDB18333zzk01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)). Additional terms and conditions: The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by Dr. Koichi Kawakami of the National Institute of Genetics (Urasaki,A. et al., Genetics 174, 639-649 (2006); Kawakami, K. et al., Developmental Cell 7: 133-144 (2004))is requested.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020))。 付加的提供条件： Tol2 systemは、国立遺伝学研究所が特許を保有している。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。利用者は、研究成果の公表にあたって国立遺伝学研究所 川上浩一博士が指定する文献 (Urasaki,A. et al., Genetics 174, 639-649 (2006); Kawakami, K. et al., Developmental Cell 7: 133-144 (2004)) の引用を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18333_B0Gfp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 (Pr0164) Region: OsTIR1 (WT) 5' Sequence file: RDB18333_B0Gfa.seq
>18333_18333_B0Gf_3_pAxCA_F1_A03_03_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNTTCN NNNNNNTCCT GGGCAACGNN    61 NNNGTTATTG TGCTGTCTCA TCATTTTGGC AAAGAATTCC TCGAAGCGCT GCCACCATGA   121 CATACTTTCC TGAAGAGGTC GTCGAACACA TTTTTAGCTT CCTGCCTGCA CAGAGAGATA   181 GAAACACAGT GAGCCTGGTC TGCAAAGTGT GGTACGAGAT CGAACGCCTG AGCCGGAGAG   241 GAGTGTTCGT CGGCAACTGC TATGCTGTGA GAGCAGGCAG GGTCGCCGCT AGGTTTCCAA   301 ATGTGCGCGC ACTGACCGTC AAGGGGAAAC CCCACTTCGC CGACTTTAAC CTGGTGCCCC   361 CTGATTGGGG AGGATACGCC GGCCCTTGGA TCGAGGCAGC CGCTCGCGGC TGTCATGGAC   421 TGGAGGAACT GCNCATGAAG CGAATGGTGG TCTCTGACNA AAGNCTGGAN CTGCTGGCTC   481 GGANNTNNCC TAGGNNNCNN GCACTGGTGC NNNNNNCTTG CNNAGGCTTC NNCACCNANG 541 GNCTGGCNNC NNNNNNNNNN //
Primer: OsTIR1-B_R (Pr1085) Region: OsTIR1 (WT) 3', P2A,mAID,EGFP 5' Sequence file: RDB18333_B0Gfb.seq
>18333_18333_B0Gf_3_OsTIR1-B_R_B03_06_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNAT GGANGAAANC NTGGCGACCT GCCNAAGGTG GAGAAACTGT    61 ATGTGTATCG GACCACCGCA GGGGCAAGAG ATGATGCTCC CAACTTTGTG AAGATTCTGA   121 GATCAGGAAG CGGAGCTACT AACTTCAGCC TGCTGAAGCA GGCTGGAGAC GTGGAGGAGA   181 ACCCTGGACC TAGATCTAAG GAGAAGAGTG CTTGTCCTAA AGATCCAGCC AAACCTCCGG   241 CCAAGGCACA AGTTGTGGGA TGGCCACCGG TGAGATCATA CCGGAAGAAC GTGATGGTTT   301 CCTGCCAAAA ATCAAGCGGT GGCCCGGAGG CGGCGGCGTT CGTGAAGGTA TCAATGGACG   361 GAGCACCGTA CTTGAGGAAA ATCGATTTGA GGATGTATAA AGCTAGCATG GTGAGCAAGG   421 GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG   481 GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC   541 TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACNC   601 TGACCTACNG CGTGCAGTGC TTCNGCCGCT ACCCCGACCA CNTGAANCAG CACGACTTCT   661 TCNAGTCCGC CATGCCCGNN NNTACGTNNN GNANCGCACC ATCNTCNTCA NGNNNNNNGC 721 NNNTNCANNN NCCGCNNNNA NNNNNANNTN NNNNNNNNN //
Primer: EGFP-C2 (Pr0402) Region: EGFP 3', Linker, NLS, SV40 pA, loxP,mPGK pro Sequence file: RDB18333_B0Gfc.seq
>18333_18333_B0Gf_3_EGFP-C2_C01_07_ABI24.ab1     1 NNNNNNNNNN NCGCNNNCNC NTGGTCCTGC TGGANTTCGT GACCGCCGCC GGGATCACTC    61 TCGGCATGGA CGAGCTGTAC AAGGGTGCAG GCGCCCAAGC TTCGAATTCT GCAGTCGACC   121 CCAAGAAGAA AAGAAAGGTC ATCGACGGTA CCGCGGGCCC GGGATCCACC GGATCTAGAT   181 AACTGATCAT AATCAGCCAT ACCACATTTG TAGAGGTTTT ACTTGCTTTA AAAAACCTCC   241 CACACCTCCC CCTGAACCTG AAACATAAAA TGAATGCAAT TGTTGTTGTT AACTTGTTTA   301 TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT   361 TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TAAGCGATCA   421 TATTCAATAA CCCTTAATAT AACTTCGTAT AATGTATGCT ATACGAAGTT ATTAGGTCTG   481 AAGAGGAGTT TACGTCCAGC CAAGCTTAGG ATCTCGACCT CGAAATTCTA CCGGGTAGGG   541 GAGGCGCTTT TCCCAAGGCA GTCTGGAGCA TGCGCTTTAG CAGCCCCGCT GGGCACTTGN   601 CGCTACACAA GTGGCCTCTG GCCTCGCACA CATTCCACAT CCACCGGTAN GCGCCAACCG   661 ACTCCGTTCN TTGGNGGNCC CNTCNNNCCN CCTTCNACTC CNCCCNNAGT NNNNNAGNNN 721 CCNCCNNNNN NNNNN //
Primer: pUC-M13_-100 (Pr0310) Region: Tol2R175, loxP, mPGK ter Sequence file: RDB18333_B0Gfd.seq
>18333_18333_B0Gf_3_pUC-M13_-100_E01_13_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN GGGNNNNGCN NGNGTTTTCC CNGTCACGAC GTTGTAAAAC    61 GACGGCCAGT GAGCGCGCGT AATACGACTC ACTATAGGGC GAATTGGGTA CCGGCATATG   121 GTTCTTGACA GAGGTGTAAA AAGTACTCAA AAATTTTACT CAAGTGAAAG TACAAGTACT   181 TAGGGAAAAT TTTACTCAAT TAAAAGTAAA AGTATCTGGC TAGAATCTTA CTTGAGTAAA   241 AGTAAAAAAG TACTCCATTA AAATTGTACT TGAGTATTAA GGAAGTAAAA GTAAAAGCAA   301 GAAAGATCTT AGTGAACCTC TTCGAGGGAC CTAATAACTT CGTATAGCAT ACATTATACG   361 AAGTTATATT AAGGGTTCCG GATCTCGACC AGCTTCTGAT GGAATTAGAA CTTGGCAAAA   421 CAATACTGAG AATGAAGTGT ATGTGGAACA GAGGCTGCTG ATCTCGTTCT TCAGGCTATG   481 AAACTGACAC ATTTGGAAAC CACAGTACTT AGAACCACAA AGTGGGAATC AAGAGAAAAA   541 CAATGATCCC ACGAGAGATC TATAGATCTA TAGATCATGA GTGGGAGGAA TGAGCTGGCC   601 CTTAATTTGG TTTTGNTTGT TTAAATTATG ATATCCAACT ATGAAACATT ATCNTNNNCN   661 ATAGTAAAGA NCCTTCNGTA AAGAGCANGC NNTTATCNNN TCCCACCCCN NNCCCCNCCC 721 CCNNNANNNC CNANNCNNNN NNTNNNAANN NNN //
Primer: PGKpro_F (Pr0249) Region: PuroR 5' Sequence file: RDB18333_B0Gfe.seq
>18333_18333_B0Gf_3_PKGpro_F_F01_16_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNTCTCCG GGCCTTTCGA CCTGCATCCN TCTAGATCTC    61 GAGCAGCTGA AGCTTACCAT GACCGAGTAC AAGCCCACGG TGCGCCTCGC CACCCGCGAC   121 GACGTCCCCA GGGCCGTACG CACCCTCGCC GCCGCGTTCG CCGACTACCC CGCCACGCGC   181 CACACCGTCG ATCCGGACCG CCACATCGAG CGGGTCACCG AGCTGCAAGA ACTCTTCCTC   241 ACGCGCGTCG GGCTCGACAT CGGCAAGGTG TGGGTCGCGG ACGACGGCGC CGCGGTGGCG   301 GTCTGGACCA CGCCGGAGAG CGTCGAAGCG GGGGCGGTGT TCGCCGAGAT CGGCCCGCGC   361 ATGGCCGAGT TGAGCGGTTC CCGGCTGGCC GCGCAGCAAC AGATGGAAGG CCTCCTGGCG   421 CCGCACCGGC CCAAGGAGCC CGCGTGGTTC CTGGCCACCG TCGGCGTCTC GCCCGACCAC   481 CAGGGCAAGG GTCTGGGCAG CGCCGTCGTG CTCCCCGGAG TGGAGGCGGC CGANCGCGCC   541 GGGNNGNCCG CCTTCCTGGA NACCTCCGCG CCCCACANCC NNCCCNTTCT ACGAGCGGCT   601 CGGNTTNCNC NNNNNNCNNC GANGTCNAGG NGNNCCNANN GNNCNNNNNN CTGGTGCATG   661 ANNCNNNNAG NCNNNTGCCT GNNNNCCGCC NCANNNNCCG NNNCGNCCNG NNNNNANNNN 721 NNNNNN //
Primer: reverse-2 (Pr0100) Region: T3 pro,Tol2L200, CMV en. Sequence file: RDB18333_B0Gff.seq
>18333_18333_B0Gf_3_reverse-2_D01_10_ABI24.ab1     1 NNNNNNNNNN NNNNNNNCGC GCNNTTAACC CTNACTAAAG GGAACAAAAG CTGGAGCTCG    61 GATCTGCTGG GCTTGCTGAA GGTAGGGGGT CAAGAACCAG AGGTGTAAAG TACTTGAGTA   121 ATTTTACTTG ATTACTGTAC TTAAGTATTA TTTTTGGGGA TTTTTACTTT ACTTGAGTAC   181 AATTAAAAAT CAATACTTTT ACTTTTACTT AATTACATTT TTTTAGAAAA AAAAGTACTT   241 TTTACTCCTT ACAATTTTAT TTACAGTCAA AAAGTACTTA TTTTTTGGAG ATCACTTGGG   301 CCCTCTAGAT GCATGCTCGA CATTGATTAT TGACTAGTTA TTAATAGTAA TCAATTACGG   361 GGTCATTAGT TCATAGCCCA TATATGGAGT TCCGCGTTAC ATAACTTACG GTAAATGGCC   421 CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC AATAATGACG TATGTTCCCA   481 TAGTAACGCC AATAGGGACT TTCCATTGAC GTCAATGGGT GGAGTATTTA CGGTAAACTG   541 CCCACTTGGC AGTACATCAA GTGTATCATA TGCCAAGTAC GCCCCCTATT GACGTCNATG   601 ACGGTAAATG GCCCGCCTGG CNTTATGCCC AGTACATGAN CTTATGGGAN TTTCCTACTT   661 GGCAGTACAT CTACNTANTA NTCNTCGCTA NNANNNNGGN CNANGTGAGC CCCNCNNTNN 721 NGNNTNNNNN CNCCCNNNNN NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

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