Please review the QC test results indicated by icon below as well as clone information before placing your order.

pAY19

TOL2 expression vector of OsTIR1(F74A) and mAID-EGFP-NLS marker.

Sequence (full)	RDB18335zzk01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)). Additional terms and conditions: The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by Dr. Koichi Kawakami of the National Institute of Genetics (Urasaki,A. et al., Genetics 174, 639-649 (2006); Kawakami, K. et al., Developmental Cell 7: 133-144 (2004))is requested.

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Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020))。 付加的提供条件： Tol2 systemは、国立遺伝学研究所が特許を保有している。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。利用者は、研究成果の公表にあたって国立遺伝学研究所 川上浩一博士が指定する文献 (Urasaki,A. et al., Genetics 174, 639-649 (2006); Kawakami, K. et al., Developmental Cell 7: 133-144 (2004)) の引用を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18335_B0Gfp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 (Pr0164) Region: insert 5'(OsTIR1(F74A) 5') Sequence file: RDB18335_B0Gfa.seq
>18335_18335_B0Gf_3_pAxCA_F1_C03_09_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN CCTTCTTCTT TTTCCTACNG CTCCTGGGCA ACGTGCTGGT    61 TATTGTGCTG TCTCATCATT TTGGCAAAGA ATTCGAGCTC GGTACCTCGC GAATGCATCT   121 AGATAGCGCT GCCACCATGA CATACTTTCC TGAAGAGGTC GTCGAACACA TTTTTAGCTT   181 CCTGCCTGCA CAGAGAGATA GAAACACAGT GAGCCTGGTC TGCAAAGTGT GGTACGAGAT   241 CGAACGCCTG AGCCGGAGAG GAGTGTTCGT CGGCAACTGC TATGCTGTGA GAGCAGGCAG   301 GGTCGCCGCT AGGTTTCCAA ATGTGCGCGC ACTGACCGTC AAGGGGAAAC CCCACGCCGC   361 CGACTTTAAC CTGGTGCCCC CTGATTGGGG AGGATACGCC GGCCCTTGGA TCGAGGCAGC   421 CGCTCGCGGC TGTCATGGAC TGGAGGAACT GCGCATGAAG CGAATGGTGG TCTCTGACGA   481 AAGTCTGGAG CTGCTGGCTC GGAGCTTCCC TAGGTTTCGC GCACTGGTGC TGATTTCTTG   541 CGAAGGCTTC AGCACCGATG GACTGGCAGC CGTGGCCTCC CACTGTAAGC TGCTGCGGGA   601 GCTGGACCTC CNGGANAATG AAGTGGANGA NAGAGGNCCC AGANGGCTGT CTTGCTTCCC   661 NNANTCNTGT ACNNGCCNNG NGTCCNGNAC TTTGNNNGNN NCAANNNNAA NNGNATGNTG 721 GGNNCNTGNN NCNNCNNGNN NNNNNNNN //
Primer: OsTIR1-B_R (Pr1085) Region: insert mid(OsTIR1(F74A) 3',P2A,mAID,EGFP 5') Sequence file: RDB18335_B0Gfb.seq
>18335_18335_B0Gf_3_OsTIR1-B_R_D03_12_ABI24.ab1     1 NNNNNNNNNN NNNNNNGGAN GANNNCATGG CGACCTGCCN AAGGTGGAGA AACTGTATGT    61 GTATCGGACC ACCGCAGGGG CAAGAGATGA TGCTCCCAAC TTTGTGAAGA TTCTGAGATC   121 AGGAAGCGGA GCTACTAACT TCAGCCTGCT GAAGCAGGCT GGAGACGTGG AGGAGAACCC   181 TGGACCTAGA TCTAAGGAGA AGAGTGCTTG TCCTAAAGAT CCAGCCAAAC CTCCGGCCAA   241 GGCACAAGTT GTGGGATGGC CACCGGTGAG ATCATACCGG AAGAACGTGA TGGTTTCCTG   301 CCAAAAATCA AGCGGTGGCC CGGAGGCGGC GGCGTTCGTG AAGGTATCAA TGGACGGAGC   361 ACCGTACTTG AGGAAAATCG ATTTGAGGAT GTATAAAGCT AGCATGGTGA GCAAGGGCGA   421 GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA   481 CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA   541 GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGNCCC ACCCTCGTGA CCACCCTGAC   601 CTACNGCGTG CAGTGCTTCN GCCGCTACCC CGACCACATG AAGCAGCNCG ACTTCNTNNA   661 GTCCGCCNTG CCCNANNNTA CNTNCAGGAN CGCACCATCN NCTNNNNNNN NNANNNNNTA 721 CNNNANCNNN NNNNANNNNN NNNNN //
Primer: EGFP-C2 (Pr0402) Region: insert mid(EGFP 3',Linker,NLS,SV40 pA,loxP,PGK pro) Sequence file: RDB18335_B0Gfc.seq
>18335_18335_B0Gf_3_EGFP-C2_C02_08_ABI24.ab1     1 NNNNNNNNNN NNCGCGANCN CNTGGTCCTG CTGGANTTCG TGACCGCCGC CGGGATCACT    61 CTCGGCATGG ACGAGCTGTA CAAGGGTGCA GGCGCCCAAG CTTCGAATTC TGCAGTCGAC   121 CCCAAGAAGA AAAGAAAGGT CATCGACGGT ACCGCGGGCC CGGGATCCAC CGGATCTAGA   181 TAACTGATCA TAATCAGCCA TACCACATTT GTAGAGGTTT TACTTGCTTT AAAAAACCTC   241 CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT TAACTTGTTT   301 ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA   361 TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTAAGCGATC   421 ATATTCAATA ACCCTTAATA TAACTTCGTA TAATGTATGC TATACGAAGT TATTAGGTCT   481 GAAGAGGAGT TTACGTCCAG CCAAGCTTAG GATCTCGACC TCGAAATTCT ACCGGGTAGG   541 GGAGGCGCTT TTCCCAAGGC AGTCTGGAGC ATGCGCTTTA GCAGCCCCGC TGGGCACTTG   601 GCGCTACACA AGTGGCCTCT GGCCTCGCAC ACATTCNNCA TCNACCGGTA NGCGCCNNCC   661 GANTCCGTNC NTTGGTGGCC CCNTNNCGCC NNCNTCNACT NNTCCCNNAN NCNGGANNNT 721 NCCCCCCNNN CCNNNNNNNN NN //
Primer: pUC-M13_-100 (Pr0310) Region: T7 pro,Tol2R175,insert 3'(loxP, mPGK ter) Sequence file: RDB18335_B0Gfd.seq
>18335_18335_B0Gf_3_pUC-M13_-100_E02_14_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNG GNANGCCNGG GTTTTCCCNG TCACGACGTT GTAAAACGAC    61 GGCCAGTGAG CGCGCGTAAT ACGACTCACT ATAGGGCGAA TTGGGTACCG GCATATGGTT   121 CTTGACAGAG GTGTAAAAAG TACTCAAAAA TTTTACTCAA GTGAAAGTAC AAGTACTTAG   181 GGAAAATTTT ACTCAATTAA AAGTAAAAGT ATCTGGCTAG AATCTTACTT GAGTAAAAGT   241 AAAAAAGTAC TCCATTAAAA TTGTACTTGA GTATTAAGGA AGTAAAAGTA AAAGCAAGAA   301 AGATCTTAGT GAACCTCTTC GAGGGACCTA ATAACTTCGT ATAGCATACA TTATACGAAG   361 TTATATTAAG GGTTCCGGAT CTCGACCAGC TTCTGATGGA ATTAGAACTT GGCAAAACAA   421 TACTGAGAAT GAAGTGTATG TGGAACAGAG GCTGCTGATC TCGTTCTTCA GGCTATGAAA   481 CTGACACATT TGGAAACCAC AGTACTTAGA ACCACAAAGT GGGAATCAAG AGAAAAACAA   541 TGATCCCACG AGAGATCTAT AGATCTATAG ATCATGAGTG GGAGGAANGA GCTGGCCCTT   601 AATTTGNTTT TGCTTGTTTA AATTATGANA TCCNACTATG AAACATTATC ATAAAGCAAT   661 AGTAAAGAGC NNTNAGTAAA NAGCNNNNTT TATCNNATCC CNCCCNNCCC NNNCCCNNAN 721 CTNNNNNCNN NNNNNNNN //
Primer: PGKpro_F (Pr0249) Region: insert mid(PuroR) Sequence file: RDB18335_B0Gfe.seq
>18335_18335_B0Gf_3_PKGpro_F_F02_17_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNTT NNNNNTCTCC GGGCCTTTCG ACCTGCATCC NTCTAGATCT    61 CGAGCAGCTG AAGCTTACCA TGACCGAGTA CAAGCCCACG GTGCGCCTCG CCACCCGCGA   121 CGACGTCCCC AGGGCCGTAC GCACCCTCGC CGCCGCGTTC GCCGACTACC CCGCCACGCG   181 CCACACCGTC GATCCGGACC GCCACATCGA GCGGGTCACC GAGCTGCAAG AACTCTTCCT   241 CACGCGCGTC GGGCTCGACA TCGGCAAGGT GTGGGTCGCG GACGACGGCG CCGCGGTGGC   301 GGTCTGGACC ACGCCGGAGA GCGTCGAAGC GGGGGCGGTG TTCGCCGAGA TCGGCCCGCG   361 CATGGCCGAG TTGAGCGGTT CCCGGCTGGC CGCGCAGCAA CAGATGGAAG GCCTCCTGGC   421 GCCGCACCGG CCCAAGGAGC CCGCGTGGTT CCTGGCCACC GTCGGCGTCT CGCCCGACCA   481 CCAGGGCAAG GGTCTGGGCA GCGCCGTCGT GCTCCCCGGA GTGGAGGCGG CCGAGCGCGC   541 CGGGGTGCCC GCCTTCCTGG AGACCTCCGC GCCCCACAAC CTCCCCTTCT ACGAGCGGCT   601 CGGCTTCANN GTCACCGCCG ANGTCGAGGT GCCCGANNGN NCNNGCACCT GGTGNNTGNN   661 CCNNNNGCCC GGTGCCTGAN GCCCGCCNCA NGANCCGNNN CGCCCGANCN AANNNANNNC 721 NNNNNNCCCN NGCATCNNNN NNNNNN //
Primer: reverse-2 (Pr0100) Region: T3 pro,Tol2L200, CMV en 5' Sequence file: RDB18335_B0Gff.seq
>18335_18335_B0Gf_3_reverse-2_D02_11_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNGC GCANTTAACC CTCACTAAAG GGAACAAAAG CTGGAGCTCG    61 GATCTGCTGG GCTTGCTGAA GGTAGGGGGT CAAGAACCAG AGGTGTAAAG TACTTGAGTA   121 ATTTTACTTG ATTACTGTAC TTAAGTATTA TTTTTGGGGA TTTTTACTTT ACTTGAGTAC   181 AATTAAAAAT CAATACTTTT ACTTTTACTT AATTACATTT TTTTAGAAAA AAAAGTACTT   241 TTTACTCCTT ACAATTTTAT TTACAGTCAA AAAGTACTTA TTTTTTGGAG ATCACTTGGG   301 CCCTCTAGAT GCATGCTCGA CATTGATTAT TGACTAGTTA TTAATAGTAA TCAATTACGG   361 GGTCATTAGT TCATAGCCCA TATATGGAGT TCCGCGTTAC ATAACTTACG GTAAATGGCC   421 CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC AATAATGACG TATGTTCCCA   481 TAGTAACGCC AATAGGGACT TTCCATTGAC GTCAATGGGT GGAGTATTTA CGGTAAACTG   541 CCCACTTGGC AGTACATCAA GTGTATCATA TGCCAAGTAC GCCCCCTATT GACGTCAATG   601 ACGGTAAATG GCCCNNCTGG CATTATGCCC AGTACATGAC CTTATGGGNN TTTCCNACNN   661 NGNANTACAT CTACGTATTA NTCATCGCTA TTANNNNGGT CNANNTGANC CCCACGTTCT 721 GCTTNNNTCT NCCNNNNNNC CCCCCCCNNN NNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl