Please review the QC test results indicated by icon below as well as clone information before placing your order.

pAAV-hSyn-OsTIR1(F74G)

Adeno-associated virus (AAV) expression vector of OsTIR1(F74G) and mAID-EGFP-NES marker with hSyn promoter.

Sequence (full)	RDB18339zzk01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)).
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020))。
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Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18339_B0Gfp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: OsTIR1-N_R1 (Pr0890) Region: insert 5'(OsTIR1(F74G),hSyn pro) Sequence file: RDB18339_B0Gfa.seq
>D07105A7_B0Gf_1_OsTIR1-N_R1_A11_02_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNTT NNNNTCTCTC TGTGCNGGCA GGAAGCTAAA AATGTGTTCG    61 ACGACCTCTT CAGGAAAGTA TGTCATGGTG GCGATCCTCT CGACTGCGCT CTCAGGCACG   121 ACACGACTCC TCCGCTGCCC ACCGCAGACT GAGGCAGCGC TGAGTCGCCG GCGCCGCAGC   181 GCAGATGGTC GCGCCCGTGC CCCCCTATCT CGCGCCTCGC GTGGTGCGGT CCGGCTGGGC   241 CGGCGGCGGC GCGGACGCGA CCAAGGTGGC CGGGAAGGGG AGTTTGCGGG GGACCGGCGA   301 GTGACGTCAG CGCGCCTTCA GTGCTGAGGC GGCGGTGGCG CGCGCCGCCA GGCGGGGGCG   361 AAGGCACTGT CCGCGGTGCT GAAGCTGGCA GTGCGCACGC GCCTCGCCGC ATCCTGTTTC   421 CCCTCCCCCT CTCTGATAGG GGATGCGCAA TTTGGGGAAT GGGGGTTGGG TGCTTGTCCA   481 GTGGGTCGGG GTCGGTCGTC AGGTAGGCAC CCCCACCCCG CCTCATCCTG GTCCTAAAAC   541 CCACTTGCAC TCATACGCAG GGCCCTCTGC AGTCTAGACA CACGCGTGCG GCCGCAGGAA 601 CCCCTAGTGA TGGAGTTGGC CACTCCCTCT CTGCGCGCTN //
Primer: OsTIR1_R3 (Pr1072) Region: insert mid(OsTIR1(F74G)) Sequence file: RDB18339_B0Gfb.seq
>18339_18339_B1KhF_1_OsTIR1_R3_C01_07_ABI24.ab1     1 NNNNNNNNNN TTNNNTTCNC CTTNGNNGCN GGCAAAGTTC AGGGNCACCA GGCTGGTACA    61 TGAGTCTGGG AAGCAAGACA GCCATCTGGG GCCTCTATCC TCCACTTCAT TCTCCTGGAG   121 GTCCAGCTCC CGCAGCAGCT TACAGTGGGA GGCCACGGCT GCCAGTCCAT CGGTGCTGAA   181 GCCTTCGCAA GAAATCAGCA CCAGTGCGCG AAACCTAGGG AAGCTCCGAG CCAGCAGCTC   241 CAGACTTTCG TCAGAGACCA CCATTCGCTT CATGCGCAGT TCCTCCAGTC CATGACAGCC   301 GCGAGCGGCT GCCTCGATCC AAGGGCCGGC GTATCCTCCC CAATCAGGGG GCACCAGGTT   361 AAAGTCGGCG CCGTGGGGTT TCCCCTTGAC GGTCAGTGCG CGCACATTTG GAAACCTAGC   421 GGCGACCCTG CCTGCTCTCA CAGCATAGCA GTTGCCGACG AACACTCCTC TCCGGCTCAG   481 GCGTTCGATC TCGTACCACA CTTTGCAGAC CAGGCTCACT GTGTTTCTAT CTCTCTGTGC   541 AGGCAGGAAG CTAAAAATGT GTTCGACGAC CTCTTCAGGA AAGTATGTCA TGGTGNCGAT   601 CCTCTCGACT GCGCTCTCAN GCACGACACG ACTCCTCCGC TGCCCACCGC AGANTGANGC   661 AGCGCTGANT CGCCNGCGCC NCANCGCANA NGGNCGCGCC NNNNCCCCCC NNANCTCNNN 721 NNNNNNN //
Primer: Venus-N_R (Pr0341) Region: insert mid(EGFP 5',mini-AID,P2A,OsTIR1 3') Sequence file: RDB18339_B0Gfc.seq
>D07105A7_B0Gf_1_Venus-N_R_D11_11_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NTTGTGGNCG TTNANGTCGC CGTCCNGCTC GACCNGGATG    61 GGCACCACCC CGGTGAACAG CTCCTCGCCC TTGCTCACCA TGCTAGCTTT ATACATCCTC   121 AAATCGATTT TCCTCAAGTA CGGTGCTCCG TCCATTGATA CCTTCACGAA CGCCGCCGCC   181 TCCGGGCCAC CGCTTGATTT TTGGCAGGAA ACCATCACGT TCTTCCGGTA TGATCTCACC   241 GGTGGCCATC CCACAACTTG TGCCTTGGCC GGAGGTTTGG CTGGATCTTT AGGACAAGCA   301 CTCTTCTCCT TAGATCTAGG TCCAGGGTTC TCCTCCACGT CTCCAGCCTG CTTCAGCAGG   361 CTGAAGTTAG TAGCTCCGCT TCCTGATCTC AGAATCTTCA CAAAGTTGGG AGCATCATCT   421 CTTGCCCCTG CGGTGGTCCG ATACACATAC AGTTTCTCCA CCTTTGGCAG GTCGCCATGA   481 TTTTCCTCCA TTTCGTTAGA CCCGTCCCGC TCATTAATGA CTTCCACGTT CAGCATAGGC   541 ATTTTACTAG CCAGGACCTG ACAGCCCTTC AGAGTCACAT TGCAACTAGA CATCCACAGG   601 CTCCTCATTG TCTCGTATCT GGCAAAGTTT CCCAGCAGAG CGGCATCCCC GAAAGGGCTG   661 TCCCGGATTT CCAGTTTTCG CAGATTCTTA CACCCGTTCA TCACGTGCAT CATGCCCTTA   721 TCGGAGTCTC CGGCAAATGC AATGCTCAGC ATCTCCAGCT GCTTAGCATA CTTGCCGATG   781 TACATGAACA CTTTGTCTGT CAGCAGGCCG GAAATGCTCA GCCTCTGGAG TCCNTTGCAC   841 TCTCTCACGA TAGCTCCNAA CCCTTCATCC AGNGGCTGGG ANGTGANCAC GTCGGGTTTG   901 CCNGGTTCCA NAATACACAG CCNAANTNGG GNGAAATTAN GGNANTTCTN GGCGANNNTC   961 ACCANNGNAG CATTANNCAT CTGANGNNAN AAATACAGCN NNNNNTCAGC TTNGGGNATC 1021 CNNNNGANAN NNNNNNNNN //
Primer: mVenus-M_F (Pr0574) Region: insert 3'(EGFP 3',NLS,SV40 pA),WPRE Sequence file: RDB18339_B0Gfd.seq
>D07105A7_B0Gf_1_mVenus-M_F_B11_05_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNC NNGGNNNNNN TCANGANCCG CNCNCAACAT    61 CGAGGANGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG AACACCCCCA TCGGCGACGG   121 CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG TCCGCCCTGA GCAAAGACCC   181 CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG GGATCACTCT   241 CGGCATGGAC GAGCTGTACA AGGGTGCAGG CGCCCAAGCT TCGAATTCTG CAGTCGACCT   301 GCCCCCCCTG GAGCGCCTGA CCCTGATCGA CGGTACCGCG GGCCCGGGAT CCACCGGATC   361 TAGATAACTG ATCATAATCA GCCATACCAC ATTTGTAGAG GTTTTACTTG CTTTAAAAAA   421 CCTCCCACAC CTCCCCCTGA ACCTGAAACA TAAAATGAAT GCAATTGTTG TTGTTAACTT   481 GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA   541 AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTAAAT   601 TCGATATCAA GCTTATCGAT AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG   661 GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT   721 ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGNTTGC   781 TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGNGGTG TGCACTGTGT   841 TTGCTGACGC NNCCCCCACT GGTTGGGGCA TTGCCACCAC CNGTCAGCNN CTTTCCNGGA   901 NTTNNGCTTT CCCCCTNCNT ATTGNCACGG CGNAACTCAT CGNNGCCTGN CTNGNCCNCT   961 GCNNGNNNNN GGGGCTCNGN NTGTNNGGNA CTNNNNNTTC NNNNNNNNNG NNGGNNAATC 1021 ATCNNNNNTN NCNNNNNNNN NN //
Primer: f1ori_F (Pr0280) Region: f1 ori,AAV2 ITR Sequence file: RDB18339_B0Gfe.seq
>18339_18339_B1KhF_1_f1ori_F_G02_20_ABI24.ab1     1 NNNNNNNNNN NNNNNGNANN GTAGCGGTCA CGCTGCGCGT AACCACCACA CCCGCCGCGC    61 TTAATGCGCC GCTACAGGGC GCGTACTATG GTTGCTTTGA CGTATGCGGT GTGAAATACC   121 GCACAGATGC GTAAGGAGAA AATACCGCAT CAGGCGCCCC TGCAGGCAGC TGCGCGCTCG   181 CTCGCTCACT GAGGCCGCCC GGGCAAAGCC CGGGCGTCGG GCGACCTTTG GTCNCCCGGC 241 CTCNNNGANN NNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.03.26

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