Please review the QC test results indicated by icon below as well as clone information before placing your order.

pAAV-hSyn-OsTIR1(F74A)

Adeno-associated virus (AAV) expression vector of OsTIR1(F74A) and mAID-EGFP-NES marker with hSyn promoter.

Sequence (full)	RDB18340zzk01.seq provided by the depositor

Distribution information

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Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)).
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020))。
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Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18340_B0Gfp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: OsTIR1_R2 (Pr0891) Region: OsTIR1 5',ccvOsTIR1 codon plus (F74A),hSyn pro Sequence file: RDB18340_B0Gfa.seq
>18340_18340_B0Gf_1_OsTIR1-N_R2_G01_07_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNATTNNCT TCGCCNTTGN NGCNNGCAAN GTTCNNGGNN    61 NCCANGCTGG TACATGANTC TGGNNANCAA GACAGCCATC TGGGGCCTCT ATCCTCCACT   121 TCATTCTCCT GGAGGTCCAG CTCCCGCAGC AGCTTACAGT GGGAGGCCAC GGCTGCCAGT   181 CCATCGGTGC TGAAGCCTTC GCAAGAAATC AGCACCAGTG CGCGAAACCT AGGGAAGCTC   241 CGAGCCAGCA GCTCCAGACT TTCGTCAGAG ACCACCATTC GCTTCATGCG CAGTTCCTCC   301 AGTCCATGAC AGCCGCGAGC GGCTGCCTCG ATCCAAGGGC CGGCGTATCC TCCCCAATCA   361 GGGGGCACCA GGTTAAAGTC GGCGGCGTGG GGTTTCCCCT TGACGGTCAG TGCGCGCACA   421 TTTGGAAACC TAGCGGCGAC CCTGCCTGCT CTCACAGCAT AGCAGTTGCC GACGAACACT   481 CCTCTCCGGC TCAGGCGTTC GATCTCGTAC CACACTTTGC AGACCAGGCT CACTGTGTTT   541 CTATCTCTCT GTGCAGGCAG GAAGCTAAAA ATGTGTTCGA CGACCTCTTC AGGAAAGTAT   601 GTCATGGTGG CGATCCTCTC GACTGCGCTC TCAGGCACGA CACGACTCCT CCGCTGCCCA   661 CCGCAGACTG AGGCAGCGCT GAGTCGCCGG CGCCGCAGCG CAGATGGTCG CGCCCGTGCC   721 CCCCTATCTC GCGCCTCGCG TGGTGCGGTC CGGCTGGGCC GGCGGCGGCG CGNANGCNAN   781 CAANGTGGCC GGGAAGGNNN NNNTGNGNNG NANNGGCGAG TGANNTCNNC GCGCCNTCAG 841 TGNTGAGGNN NNNNNGNNNN NNNNNN //
Primer: EGFP-N_R2 (Pr0905) Region: EGFP 3',NES Sequence file: RDB18340_B0Gfb.seq
>18340_18340_B0Gf_1_EGFP-N_R2_B01_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNG NNNANNNNNG CNCGCCGTAN GTCAGGGTGG TCACGAGGGT    61 GGGCCAGGGC ACGGGCAGCT TGCCGGTGGT GCAGATGAAC TTCAGGGTCA GCTTGCCGTA   121 GGTGGCATCG CCCTCGCCCT CGCCGGACAC GCTGAACTTG TGGCCGTTTA CGTCGCCGTC   181 CAGCTCGACC AGGATGGGCA CCACCCCGGT GAACAGCTCC TCGCCCTTGC TCACCATGCT   241 AGCTTTATAC ATCCTCAAAT CGATTTTCCT CAAGTACGGT GCTCCGTCCA TTGATACCTT   301 CACGAACGCC GCCGCCTCCG GGCCACCGCT TGATTTTTGG CAGGAAACCA TCACGTTCTT   361 CCGGTATGAT CTCACCGGTG GCCATCCCAC AACTTGTGCC TTGGCCGGAG GTTTGGCTGG   421 ATCTTTAGGA CAAGCACTCT TCTCCTTAGA TCTAGGTCCA GGGTTCTCCT CCACGTCTCC   481 AGCCTGCTTC AGCAGGCTGA AGTTAGTAGC TCCGCTTCCT GATCTCAGAA TCTTCACAAA   541 GTTGGGAGCA TCATCTCTTG CCCCTGCGGT GGTCCGATAC ACATACAGTT TCTCCACCTT   601 TGGCAGGTCG CCATGATTTT CCTCCATTTC GTTAGACCCG TCCCGCTCAT TAATGACTTC   661 CACGTTCAGC ATAGGCATTT TACTAGCCAG GACCTGACAG CCCTTCAGAG TCACATTGCA   721 ACTAGACATC CACAGGCTCC TCATTGTCTC GTATCTGGCA AAGTTTCCCA GCAGANCGGC   781 ATCCCCGAAA GGGCTGTCCC GGATTTCCAG TTTTCGCAGA NTCTTACACC CGTTCATCAC   841 GTGCATCATG CCCTTATCGG ANTCTCCGGC AAATGCAATG CTCAGCATCT CCAGCTGCTN   901 ANCATACNNG CCNANGTACA TGAACACTTT GTCTGTCAGC NNNNCNGAAN NGCTCANCCN 961 NNNNANNNCC NNNNNNNNNN //
Primer: EGFP-N_rev (Pr0156) Region: EGFP 5',mini-AID,P2A,OsTIR1 3' Sequence file: RDB18340_B0Gfc.seq
>18340_18340_B0Gf_1_EGFP-N_rev_E01_05_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNGN NNNNNNNNGN CGANGGCGNN GCCNCCTACG GCAAGCTGAN    61 CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC   121 CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT   181 CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA   241 CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT   301 CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA   361 CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG GCATCAAGGT   421 GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG ACCACTACCA   481 GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC   541 CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT   601 CGTGACCGCC GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGGGTG CAGGCGCCCA   661 AGCTTCGAAT TCTGCAGTCG ACCTGCCCCC CCTGGAGCGC CTGACCCTGA TCGACGGTAC   721 CGCGGGCCCG GGATCCACCG GATCTAGATA ACTGATCATA ATCAGCCATA CCACATTTGT   781 ANAGGTTTTA CTTGCTTNAA AAAACCTCCC ACACCTCCCN CTGANCCTGA NACATAAAAT   841 GAATGCAATT GNTGNTGTTA ACTTGTTTAT TGCANCTTAT AATGGTTACA AATAAAGCAA   901 TAGCATCNCA AATTTCANNA ANNNAANNCN TTTTTTTCNN TGNNTTCTAN NTNGTGGTTT   961 GNCCNAANTC ATCANNGNAT CNTNNNNNNN NATATCNANN NNTANCNANN NTCNNNCNNN 1021 NNNNNTNNNN NAANNNNNNN ANNNNNNNNN N //
Primer: EGFP-C2 (Pr0402) Region: EGFP 3',NES,SV40 pA,WPRE Sequence file: RDB18340_B0Gfd.seq
>18340_18340_B0Gf_1_EGFP-C2_C01_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNG NNCNCNTGGT CCTGCTGGAN TTCGTGANCG CCGCCGGGNT    61 CACTCTCGGC ATGGACGAGC TGTACAAGGG TGCAGGCGCC CAAGCTTCGA ATTCTGCAGT   121 CGACCTGCCC CCCCTGGAGC GCCTGACCCT GATCGACGGT ACCGCGGGCC CGGGATCCAC   181 CGGATCTAGA TAACTGATCA TAATCAGCCA TACCACATTT GTAGAGGTTT TACTTGCTTT   241 AAAAAACCTC CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT   301 TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC   361 AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC   421 TTAAATTCGA TATCAAGCTT ATCGATAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT   481 TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC   541 CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT   601 GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA   661 CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT   721 CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG   781 CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGNCGGGGA   841 ANTCATCGTC CTTTCCTTGN CTGCTCGCCT ATGTTGCNAC CTGNATTCTG CGCGGGNNGN   901 CCTTCTGCTA NGTCCCTTCG GNCCTNNANT NCANCGGACC TTNCTNNCCN GCGGNCTGNT   961 GNCGGCTCTG CGGNNNNTTN CNNNNNTNCN CNTTNNNCNN CANANNANNN NNNANNNNCC 1021 NTTNNNNNNN NCNNCCCNNN NNNNNNN //
Primer: SV40_F2 (Pr1194) Region: WPRE,bGH pA,AAV2 ITR Sequence file: RDB18340_B0Gfe.seq
>18340_18340_B0Gf_1_SV40_F2_C01_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN TTCCGCGTCT TCGCCTTCGC CCTCAGACGA GTCGGATCTC    61 CCTTTGGGCC GCCTCCCCGC ATCGATACCG AGCGCTGCTC GAGAGATCTA CGGGTGGCAT   121 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC   181 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT   241 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG   301 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA   361 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC   421 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC   481 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC   541 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTGTAG   601 GTAACCACGT GCGGACCGAG CGGCCGCAGG AACCCCTAGT GATGGAGTTG GCCACTCCCT 661 CTCTGCGCGC TCNCTCNNNN NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.03.26

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