Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMK419

A plasmid for generate yeast lines expressing OsTIR1(F74G) under the GAL promoter.

Sequence (full)	RDB18342zzk02.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)).
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020))。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18342_B0Gfp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-CAPbs_up_F2 (Pr0739) Region: insert 5'(GAL1-10 pro) Sequence file: RDB18342_B0Gfa.seq
>D07105B1_B0Gf_1_pUC-CAPbs_up_F2_E03_15_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNGCTTNNNN CTTTATGCTT CCGGCTCGTA TGTTGTGTGG    61 AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGCTAT GACCATGATT ACGCCAAGCT   121 CGAAATTAAC CCTCACTAAA GGGAACAAAA GCTGGTACCG GGCCCCCCCT CGAGGTCGAC   181 GGTATCGATA AGCTTGATAT CGAATTCGAC AGGTTATCAG CAACAACACA GTCATATCCA   241 TTCTCAATTA GCTCTACCAC AGTGTGTGAA CCAATGTATC CAGCACCACC TGTAACCAAA   301 ACAATTTTAG AAGTACTTTC ACTTTGTAAC TGAGCTGTCA TTTATATTGA ATTTTCAAAA   361 ATTCTTACTT TTTTTTTGGA TGGACGCAAA GAAGTTTAAT AATCATATTA CATGGCATTA   421 CCACCATATA CATATCCATA TACATATCCA TATCTAATCT TACTTATATG TTGTGGAAAT   481 GTAAAGAGCC CCATTATCTT AGCCTAAAAA AACCTTCTCT TTGGAACTTT CAGTAATACG   541 CTTAACTGCT CATTGCTATA TTGAAGTACG GATTAGAAGC CGCCGAGCGG GTGACAGCCC   601 TCCGAAGGAA GACTCTCCTC CGTGCGTCCT CGTCTTCACC GGTCGCGTTC CTGAAACGCA   661 GATGTGCCTC GCGCCGCACT GCTCCGAACA ATAAAGATTC TACAATACTA GCTTTTATGG   721 NTATGAAGAG GAAAAATTGG CAGTAACCTG GCCCCACAAA CCTTCAAATG AACGAATCAA   781 ATTAACAACC ATAGGANGAN AATGCGATTA GTTTTTTAGN CTTATTTCTG GGGTAATTAA   841 TCAGCGAANC GATGANTTTT GATCTATTAA CAGATATATA AATGNAAAAA CTGCNTAACC   901 ACTTTNACNN ATACTTTCAA CATTTTCNNN TTGNANTACT TCTTANTNNA ATGTAATAAA   961 AGTATCAACN AAAAANTGNT NATATACCTC TATACTTTNN NGNNNNNGAN AAAAAANCCN 1021 GNNNNNNNNA NTANGNNNNN NTTNNNNNNN NNN //
Primer: pBAC_lac_F (Pr0544) Region: insert 3'(OsTIR1(F74G) 3') Sequence file: RDB18342_B0Gfb.seq
>D07105B1_B0Gf_1_pBAC_lac_F_H07_22_ABI24.ab1     1 GNNNNNNNNN NNNNNNNNNN NNNNANGANT CNCTATAGGG CGAANTGGAN CTCCNCCGCG    61 GTGGCGGCCG CTCATAAAAT CTTGACGAAG TTAGGAGCAT CATCTCTGGC ACCTGCGGTT   121 GTTCTGTAGA CGTATAACTT TTCAACTTTA GGCAAGTCAC CATGGTTTTC TTCCATTTCA   181 TTGGAACCAT CTCTTTCGTT AATAACTTCA ACGTTCAACA TTGGCATCTT TGAAGCTAAA   241 ACTTGACAAC CTTTCAAGGT AACGTTACAA CTGGACATCC ACAAAGATCT CATTGTTTCA   301 TATCTTGCGA AATTACCCAA CAAAGCTGCG TCACCAAATG GAGAATCTCT GATTTCCAAC   361 TTTCTTAAGT TCTTACAACC GTTCATAACA TGCATCATAC CCTTATCGGA GTCACCAGCG   421 AATGCGATTG ACAACATTTC CAATTGCTTA GCGTATTTAC CGATGTACAT AAAAACCTTA   481 TCTGTCAACA AACCACTGAT GGACAATCTT TGCAAACCCT TACACTCTCT AACGATTGCA   541 CCAAAACCTT CATCTAAAGG TTGTGATGTA ACAACATCAG GTTTACCTGG TTCCAAGATA   601 CACAATCTGA ATCTAGTGAA GTTTGGACAA TTTTTAGCAA CAGTAACCAA AGCTGCGTTG   661 GTCATTTGAT GACAGAAGTA CAACAAGGAG TTCAACTTAN GACAACCCAA TGAAACTGCA   721 ACTAAACCTT CTTCTGTAAC AGCAGANTAA CCTGCAACAT AGAAATCACT TGGAAAAACT   781 CTCAATTCTT GCAAATCCTT ACAAGAAGAA NCAACAACTT GCNAACCTTT ATCTGAGATA   841 CAATCCAAAA NCCACAATCN TNGCANCTTA ACACATCTAG AGNTCATCTT TGNCAAATCA   901 CTAGCATCCA AAGTTGGNGC GTAANANAAA TTCAAANNNG TCNATNGANC ACANNANNNN 961 NATATAAAAN ANNANCNANC NGGNNNTNNN NNCNNAAANN NNNNNNNN //
Primer: GAL1pro_F (Pr0030) Region: insert mid(GAL1-10 pro,OsTIR1(F74G) 5') Sequence file: RDB18342_B0Gfc.seq
>D07105B1_B0Gf_1_GAL1pro_F2_B08_05_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNTNNNA TACCTCTATA CTTTAACGTC NAGGAGAAAA    61 AACCCCGGAT CCACTAGTAT GACTTATTTT CCTGAAGAAG TTGTAGAACA CATATTTTCC   121 TTTTTACCTG CTCAAAGAGA TAGAAACACC GTCTCCTTGG TCTGTAAGGT TTGGTACGAA   181 ATCGAAAGAT TGTCTAGAAG AGGTGTTTTT GTTGGTAATT GTTACGCAGT TAGAGCTGGT   241 AGAGTTGCTG CAAGATTCCC AAACGTTAGA GCATTGACTG TTAAAGGTAA ACCTCATGGT   301 GCTGATTTCA ATTTGGTTCC ACCTGATTGG GGTGGTTATG CTGGTCCATG GATTGAAGCT   361 GCAGCTAGAG GTTGTCACGG TTTGGAAGAA TTGAGAATGA AGAGAATGGT TGTTTCTGAT   421 GAATCATTGG AATTGTTAGC AAGATCTTTT CCTAGATTCA GAGCTTTGGT TTTGATCTCA   481 TGTGAAGGTT TTTCCACCGA TGGTTTAGCA GCTGTTGCTT CACATTGTAA GTTGTTGAGA   541 GAATTGGATT TGCAAGAAAA CGAAGTTGAA GATAGAGGTC CAAGATGGTT ATCTTGTTTT   601 CCTGATTCCT GTACAAGTTT GGTTTCTTTG AACTTCGCAT GTATCAAGGG TGAAGTTAAC   661 GCTGGTAGTT TGGAAAGATT AGTTTCTAGA TCACCAAATT TGAGATCTTT GAGATTGAAC   721 AGATCCGTTA GTGTTGATAC CTTGGCAAAG ATCTTGTTGA GAACTCCTAA TTTGGAAGAN   781 TTGGGTACTG GTAACTTGAC AGATGATTTC CAAACAGAAT CATACTTCAA GTTGACCTCC   841 GCTTTGGAAA ANTGTAAGAT GTTGAGATCT TTGTCAGGNT TTTGGGATGN AAGTNCAGTT   901 TGTTTGTCTT TTATATATCC TTTGTGTGCT CNATTGACTG NNTTGAATTT GNCTTACGCA 961 CNNNCTTTNN ATGCTANNGA NTTGANNAAG NNGATCTCTN NNNNNNNNN //
Primer: URA3pro_F (Pr0170) Region: URA3 pro, URA3 5' Sequence file: RDB18342_B0Gfd.seq
>D07105B1_B0Gf_1_URA3pro_F_H03_24_ABI24.ab1     1 NNNNNNNNNN NNNNNNANAN NNTGNNNTTG CCNNGTATTC TTAACCCNAC TGCACAGAAC    61 AAAAACCTGC AGGAAACGAA GATAAATCAT GTCGAAAGCT ACATATAAGG AACGTGCTGC   121 TACTCATCCT AGTCCTGTTG CTGCCAAGCT ATTTAATATC ATGCACGAAA AGCAAACAAA   181 CTTGTGTGCT TCATTGGATG TTCGTACCAC CAAGGAATTA CTGGAGTTAG TTGAAGCATT   241 AGGTCCCAAA ATTTGTTTAC TAAAAACACA TGTGGATATC TTGACTGATT TTTCCATGGA   301 GGGCACAGTT AAGCCGCTAA AGGCATTATC CGCCAAGTAC AATTTTTTAC TCTTCGAAGA   361 CAGAAAATTT GCTGACATTG GTAATACAGT CAAATTGCAG TACTCTGCGG GTGTATACAG   421 AATAGCAGAA TGGGCAGACA TTACGAATGC ACACGGTGTG GTGGGCCCAG GTATTGTTAG   481 CGGTTTGAAG CAGGCGGCAG AAGAAGTAAC AAAGGAACCT AGAGGCCTTT TGATGTTAGC   541 AGAATTGTCA TGCAAGGGCT CCCTATCTAC TGGAGAATAT ACTAAGGGTA CTGTTGACAT   601 TGCGAAGAGC GACAAAGATT TTGTTATCGG CTTTATTGCT CAAAGAGACA TGGGTGGAAG   661 AGATGAAGGT TACGATTGGT TGATTATGAC ACCCGGTGTG GGTTTAGATG ACAAGGGAGA   721 CGCATTGGGT CAACAGTATA GAACCGTGGA TGANGTGGTC TCTACAGGAT CTGACATTAT   781 TATTGNTGGA AGAGGANTAT TTGCAAAGGG AAGGGANGCT AANGTAGAGG GTGAANGTTA   841 CAGAAAANCA NGCTGGGAAG CATATTTGAG AAGANGCGGC CAGCAAAACT NAAAAACTGT   901 ATTATAAGTN AATGNATGTA TACTNAACTC NCAAATTANA GCTTCNNNTT NATTATATCA   961 GTTATTANCC NATGCNGTGT GNAANANCNN NNNNANGCNN NNNNNNAAAA NNNNNNCNNC 1021 NNNAANTNGN ANNCNNNNNN NTTNGNNNAA NNNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl