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pET30c-streptag-TET2 (#RDB18377)

Bacterial expression vector encoding the catalytic domain of human TET2.

Clone info. Bacterial expression vector of the codon-optimized catalytic domain of human Ten-eleven translocation 2 (TET2), composed of a Streptag at the N-terminal, amino acids corresponding to 1129-1480th aa and 1844-1936th aa of NM_001127208.3 linked with a 15-residue GS linker (GGGGSGGGGSGGGGS). See Fig.S1 of Taka, N. and Yoshida, W., Anal. Bioanal. Chem., 412 (22): 5299-5305, 2020.
Comment Expression was confirmed by the depositor with SDS-PAGE.
Vector backbone pET30c (plasmid)
Size of vector backbone 6.7 kb
Selectable markers Kanamycin
Growth conditions LB+Kan, 37degC
Gene/insert name human TET2 cDNA
Depositor|Developer Yoshida, Wataru |
 
Sequence (full) RDB18377zzk01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Taka, N. and Yoshida, W. Anal. Bioanal. Chem. 412(22): 5299-5305, 2020)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Taka, N. and Yoshida, W. Anal. Bioanal. Chem. 412(22): 5299-5305, 2020)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB18377 pET30c-streptag-TET2 Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pET30c-streptag-TET2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18377).

Reference section:

Taka, N., Yoshida, W., Quantification of global DNA methylation level using 5-methylcytosine dioxygenase. Anal. Bioanal. Chem. 412 (22): 5299-5305 (2020). PMID 32504107. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Taka, N., Quantification of global DNA methylation level using 5-methylcytosine dioxygenase. Anal. Bioanal. Chem. 412 (22): 5299-5305 (2020). PMID 32504107. [link to RRC of NBRP]
protocol Taka, N., Global DNA Methylation Analysis Using Methylcytosine Dioxygenase. In: Yuan BF. (eds) DNA Modification Detection Methods. Springer Protocols Handbooks. Humana, New York, NY. pp 93-102 (2021). [link to Journal]