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pET T7-7alpha-actinin1-SNAP-His

Bacterial expression vector of human alpha actinin1 (ACTN1).

Catalog number RDB18384
Resource name pET T7-7alpha-actinin1-SNAP-His
Clone info. Bacterial expression vector of human alpha actinin1 (ACTN1), T7 promoter. Construct; ACTN1 was truncated at the actin binding domain (Asp1-Ala247), SNAP-tag and 6x His-tag were attached at the C-terminal via linkers (3 a.a., GGL). Use Rosetta (DE3) or equivalent for production of protein.
Comment Expression was confirmed by the depositor.
Vector backbone pET T7-7 (plasmid)
Selectable markers Ampicillin
Gene/insert name human ACTN1 cDNA
Depositor|Developer Iwaki, Mitsuhiro |
Other clones in our bank

External Database
human ACTN1

          Reference sequence
            
           
          Sequence (full) RDB18384zzk01.seq provided by the depositor

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fujita, K. et al., Commun. Biol. 2: 437, 2019)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Fujita, K. et al., Commun. Biol. 2: 437, 2019)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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          RDB18384 pET T7-7alpha-actinin1-SNAP-His Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Original reference

          original Fujita, K., Direct visualization of human myosin II force generation using DNA origami-based thick filaments. Commun. Biol. 2: 437 (2019). PMID 31799438.

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Fujita, K., Direct visualization of human myosin II force generation using DNA origami-based thick filaments. Commun. Biol. 2: 437 (2019). PMID 31799438.

          2022.08.12

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