Please review the QC test results indicated by icon below as well as clone information before placing your order.

pETDuet-2B4-2B2_dL314Q

Bacterial expression vector of human eIF2Bdelta mutant (L314Q) and human eIF2Bbeta.

Distribution information

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Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Zyryanova, A.F. et al., Mol. Cell 81: 88-103, 2021)
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Zyryanova, A.F. et al., Mol. Cell 81: 88-103, 2021)。
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Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18642_B0Lbp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pBR322_BamHI_R (Pr0065) Region: lacO, RBS, insert 5' (2B4 5') Sequence file: RDB18642_B0Lba.seq
>D07570A4_B0Lb_2_pBR322_BamHI_R_A02_02_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NANNANTNNC TATANNNNAA TTGTGAGCGG ATAACAATTC    61 CCCTCTAGAA ATAATTTTGT TTAACTTTAA GAAGGAGATA TACCATGGGC AGCAGCAGCC   121 AGGATCCGAA TTCGAGCTCG GCGCGCCTGC AGGTCGACGC TGCTGTGGCC GTGGCTGTTC   181 GCGAGGACTC GGGATCCGGG ATGAAGGCGG AGCTTCCCCC CGGGCCTGGG GCAGTGGGGA   241 GGGAAATGAC CAAAGAAGAA AAGCTGCAGC TTCGGAAGGA AAAGAAACAG CAGAAGAAGA   301 AACGGAAGGA AGAAAAGGGG GCAGAACCAG AGACTGGCTC TGCTGTATCT GCAGCCCAAT   361 GTCAAGTAGG CCCAACCAGA GAACTGCCAG AATCGGGCAT TCAGTTGGGC ACTCCTCGGG   421 AGAAAGTTCC AGCTGGTCGG AGTAAGGCCG AACTTCGGGC TGAGCGTCGA GCCAAGCAGG   481 AGGCCGAGCG GGCCCTGAAA CAGGCAAGAA AAGGGGAACA AGGAGGACCA CCTCCTAAGG   541 CCAGCCCCAG CACAGCTGGA GAAACCCCCT CAGGAGTGAA GCGTCTCCCT GAGTACCCTC   601 AGGTTGATGA CCTACTTCTG AGAAGGCTTG TTAAAAAACC AGAGCGTCAA CAGGTTCCTA   661 CACGAAAGGA TTATGGATCC AAAGTCAGTC TCTTCTCTCA CCTACCCCAG TACAGCAGAC   721 AAAACTCTCT GACCCAGTTT ATGAGCATCC CATCCTCTGT GATCCACCCA GCCATGGTGC   781 GACTCGGCCT GCAGTACTCC CAGGGCCTGG TCAGTGGCTC CNATGCCCGG NGTATTGCCC   841 TGCTTCGTGN CTTGCAGCAG GTGATTCANG ATTACACNAC ACCGCCTAAT GAAGAANTCT   901 CCAGGGATCT AGTGAATAAA CTAAAACCCT ACNTGANCTT NCTGANTCAG NGNCGTCCCC   961 NGNCAGCGAG CATGNNCAAC GCCNTNNNGT TNCTTNNCNN NGAAATCANC AGNGNGGGCA 1021 NTTNCNNGNN NNNANNANNN NNANNCNGAN NTTNNNNNNN NNN //
Primer: T7 terminator (Pr0155) Region: insert 3' (2B2 3') Sequence file: RDB18642_B0Lbb.seq
>D07570A4_B0Lb_2_T7 terminator_B02_05_ABI24.ab1     1 NNNNNNNNNN NNNNNNNGCN NNNANNNGAN TCNNTGTGCT GGCGTTCAAA TTTCGCAGCA    61 GCGGTTTCTT TACCAGACTC GAGGGTACCG ACGTCTCATA AAACATGATC ATCAGGATGG   121 TAGAGTTCAC TCATCAGGCG GTAGATGTAG GAAGGTGCAT TCCCACCAAT GTTGGAGATA   181 AAGAGGGTAA TGAGCTCTGG GGGAACGTAG TCAAACACAG GGCAATGCAC GCTGACCTTC   241 TCCAGAATGT CCCCTTCTGT GAATGGCAGG ACTTCTTCAG GAGCCACAAA CTTATGAAAT   301 GAGTCTTCTT CATTGGGGAA CTGTGGAGAA AGTTTGAACA TAGGTGCACA GACGATGAGT   361 GGGGTGGAAT GGTGTTTTGC TGCCAGTGCC AGAGTGTGAG TTCCTGTCAC AGCTCTCAGG   421 GCCCCATTGG CCAGGATGGT CTTCGTGCCA ATGATCACCT TGTTGACTCT TGACATAACG   481 GCAAAAATGG CAGCATCAGT CATGACAGTT GTCTCAATAC CTGCTTTGGA CAAATTCACA   541 GCCATTTCAT GACCCTGGCA GAAAGGAGCA CACTCTGCTA CAATGACATG GAATTTCCTC   601 TTTCGGGCAG CCTCTTTGAG GAAGGCCTCT ACTGTTCGGG AGAAGCCAAT GGTCATGATC   661 ACCTCATTGG AGTGAATGTG CTCCAGAGCC TGGGCTGCAA TGTTCTCCAT TGTCCCTTCC   721 AGCTCCACTA GCAGCTCATT AATCGCCTCA ATGATGTTGG ACTGGAGTTG GGCATAATGG   781 AAGCTGAAAT CCTCGTTTAN GNCTCCGGAT GTCAACAGTT TGTGCAGGGA CTCCTGCTGN   841 TCACTCTCGT CGCTGCGTCC ATGGANTCTG CCATACTCCT CCCGGANAAT CTTGANCANT   901 CTCCGCACCA TGTTGCCCAC NGNGGTCTCN GANGNNTGAN CGGCCGTCAT CCTCCTGNCN   961 NCTCTGNNGA TCAGCTCCNT CANCTCCCCC GCGTTGNTCC NGCNGNGGNC CGNGNNGNNC 1021 TGNNNCANNN NCCCNANGGN CNCCNNNNNN NTTTNNNNNN NNNN //
Primer: hEIF2B-M_F1 (Pr0935) Region: insert mid (2B4 L314Q) Sequence file: RDB18642_B0Lbc.seq
>D07570A4_B0Lb_2_hEIF2B-M_F1_C02_08_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNCC CTCNGGANTG AAGCGTCTCC CTGAGTACCC    61 TCAGGTTGAT GACCTACTTC TGAGAAGGCT TGTTAAAAAA CCAGAGCGTC AACAGGTTCC   121 TACACGAAAG GATTATGGAT CCAAAGTCAG TCTCTTCTCT CACCTACCCC AGTACAGCAG   181 ACAAAACTCT CTGACCCAGT TTATGAGCAT CCCATCCTCT GTGATCCACC CAGCCATGGT   241 GCGACTCGGC CTGCAGTACT CCCAGGGCCT GGTCAGTGGC TCCAATGCCC GGTGTATTGC   301 CCTGCTTCGT GCCTTGCAGC AGGTGATTCA GGATTACACA ACACCGCCTA ATGAAGAACT   361 CTCCAGGGAT CTAGTGAATA AACTAAAACC CTACATGAGC TTCCTGACTC AGTGCCGTCC   421 CCTGTCAGCG AGCATGCACA ACGCCATCAA GTTCCTTAAC AAGGAAATCA CCAGTGTGGG   481 CAGTTCCAAG CGGGAAGAGG AGGCCAAGTC AGAACTTCGA GCAGCCATTG ATCGGTATGT   541 GCAAGAGAAG ATTGTGCAAG CAGCTCAGGC AATTTCACGC TTTGCTTACC AGAAGATCAG   601 TAATGGAGAT GTGATCCTGG TATATGGATG CTCATCTCTG GTATCACGAA TTCTTCAGGA   661 GGCTTGGACA GAGGGCCGGC GGTTTCGGGT GGTAGTGGTG GACAGCCGGC CATGGCTGGA   721 AGGAAGGCAC ACACTACGTT CTCTAGTCCA TGCTGGTGTC CCAGCCTCCT ACCTGCTGAN   781 TCCTGCAGCC TCCTATGTGC TCCCAGANGT TTCCNAGGTG CTATTGGGAG CTCATGCACT   841 CTTGGNCAAC GGGTCTGTGA TGTCACGGGN ANGGACAGCA CAGTTAGCCC TGGTGGCTCG   901 AGCCCATAAT GNACCAGNGC TGGNTTGCTG TGAAACATAC NAGTTCTGTG ANCGTGNGCN   961 NACTGNNGCC TTTGNCTCTA NTGANCTANN NGNNCNTGAN GATCTGNNNN GTAANCGGGG 1021 ANAANNTGNN GCNCTGGCTN NNNNGNNNAN NNNNNNNNCC CNANNNNNNN NNN //
Primer: hEIF2Bb_R (Pr0902) Region: insert mid (2B2 5', RBS, lacO, T7pro, 2B4 3') Sequence file: RDB18642_B0Lbd.seq
>D07570A4_B0Lb_2_hEIF2Bb_R_D02_11_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNCGNNCGNC NNCCTCCTGC CCTCTCTGCG GNTCAGCTCC    61 ATCAGCTCCC CCGCGTTGCT CCNGCGGTGG TCCGTGATGA TCTGGCGCAG CAACCCTAGG   121 GTCTCCCGAG CCATTTCCTC GGAGCTGCGC GGCCCACCAC CCCGCTTCAG GGTCTCCACG   181 AAGCTCTCGA TCCTCTCTGA CAACTCCGAG CCCTTCGCTG CGGATCCCGG CATATGTATA   241 TCTCCTTCTT ATACTTAACT AATATACTAA GATGGGGAAT TGTTATCCGC TCACAATTCC   301 CCTATAGTGA GTCGTATTAA TTTCGATTAT GCGGCCGTGT ACAATACGAT TACTTTCTGT   361 TCGACTTAAG CATTATGCGG CCGCTCACTG GTCACTGCTC TTGACTCGTA GAACAACAGG   421 TACAGAACTG CAAGGGATCA TCCCCAGCTC CGTGATCACC AGATCCACAA GCTCTGGGGG   481 AGTCACATCA TAGACTAGAT TCAACAACCG TAGGGATGCG TGGTTCTGCC AGTTAGCCAG   541 CGCAACATGT TCTCCCCGCT TACATTGCAG ATCATCAGGG TCATCTAGCT CATTAGAGAC   601 AAAGGCATCA GTCTGCACAC GCTCACAGAA CTTGTATGTT TCACAGCAAA CCAGCACTGG   661 TACATTATGG GCTCGAGCCA CCAGGGCTAA CTGTGCTGTC CCTACCCGTG ACATCACAGA   721 CCCGTTGGCC AAGAGTGCAT GAGCTCCCAA TAGCACCTTG GAAACCTCTG GGAGCACATA   781 NGANGCTGCA NGAATCANCA GGTANGANGC TGGGACACCA GCATGGACTA GAGAACGTAG   841 TGTGTGCCTT CCTTCCAGCC ATGGCCGGCT GTCCACCACT ACCACCCGAA NNCGCCGGCC   901 NNCTGTCNAA NCCTCCTGAA NANNTCNNGA NANCAGANNT GANCATCCAT ATACCNNNAT   961 CACATCTNCN TTANTGATCN NCTGGNANNC AAAGCNNGNA NNTGNNNGAN CTGNNNGNAC 1021 NANCTTCNNN NGNNNNTANN NATNNNNGNN NGNNCNNNNN NNNNN //
Primer: pGL4pA_R (Pr0664) Region: lacI Sequence file: RDB18642_B0Lbe.seq
>D07570A4_B0Lb_2_pGL4pA_R_E02_14_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNCNGNNGNC TAATGAGTGA GCTAACTTAC NTTAATTGCG    61 TTGCGCTCAC TGCCCGCTTT CNNGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC   121 GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCC AGGGTGGTTT TTCTTTTCAC   181 CAGTGAGACG GGCAACAGCT GATTGCCCTT CACCGCCTGG CCCTGAGAGA GTTGCAGCAA   241 GCGGTCCACG CTGGTTTGCC CCAGCAGGCG AAAATCCTGT TTGATGGTGG TTAACGGCGG   301 GATATAACAT GAGCTGTCTT CGGTATCGTC GTATCCCACT ACCGAGATGT CCGCACCAAC   361 GCGCAGCCCG GACTCGGTAA TGGCGCGCAT TGCGCCCAGC GCCATCTGAT CGTTGGCAAC   421 CAGCATCGCA GTGGGAACGA TGCCCTCATT CAGCATTTGC ATGGTTTGTT GAAAACCGGA   481 CATGGCACTC CAGTCGCCTT CCCGTTCCGC TATCGGCTGA ATTTGATTGC GAGTGAGANA   541 TTTATGCCAG CCAGCCAGAN GCAGACGCGC CGAGACAGAA CTTAATGGGN CCGCTAACAG   601 CGCGANTTGC TGGTGACCCN ATGCGANCAG ATGCTCCACG CCCAGTCGCG NANCGTCTTC   661 NTGGGANAAA ATANTACTGN TGANGGGTGN CTGGNCNNAN ANATCNANAA ATAACGNCNG   721 NNANNTTANN GNNNNANCTT NCNCANCNNT GNNATNCTNG NCNTNCNNCN NANANNNANN 781 GNTNNNCCNN NNGANNNNNN NNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl