Please review the QC test results indicated by icon below as well as clone information before placing your order.

pYC143 pBS-GPDp-Tom71-GFP1-10-CyC1ter-hphMX

The split-GFP system clone for visualizing organelle contact sites in S. cerevisiae. probe: GFP1-10; location: mitochondria.

Sequence (full)	RDB18660zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Tashiro, S. et al. Front. Cell Dev. Biol. 8(1): 6175, 2020)
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Tashiro, S. et al. Front. Cell Dev. Biol. 8(1): 6175, 2020)
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18660_B0Lnp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: f1ori_F2 (Pr0308) Region: f1 ori, T7 pro, GPD pro Sequence file: RDB18660_B0Lna.seq
>D07536A6_B0Ln_1_f1ori_F2_A07_01_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NTNNNNNNNT TCGGCCTATT GGTTAANAAA AATGAGCTGA    61 TTTAACNNAA AATTTAACGC GAATTTTAAC AAAATATTAA CGCTTACAAT TTCCATTCGC   121 CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC   181 AGCTGGCGAA AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC   241 AGTCACGACG TTGTAAAACG ACGGCCAGTG AGCGCGCGTA ATACGACTCA CTATAGGGCG   301 AATTGGGTAC CGGGCCCCCC CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTAG   361 TTTATCATTA TCAATACTCG CCATTTCAAA GAATACGTAA ATAATTAATA GTAGTGATTT   421 TCCTAACTTT ATTTAGTCAA AAAATTAGCC TTTTAATTCT GCTGTAACCC GTACATGCCC   481 AAAATAGGGG GCGGGTTACA CAGAATATAT AACATCGTAG GTGTCTGGGT GAACAGTTTA   541 TTCCTGGCAT CCACTAAATA TAATGGAGCC CGCTTTTTAA GCTGGCATCC AGAAAAAAAA   601 AGAATCCCAG CACCAAAATA TTGTTTTCTT CACCAACCAT CAGTTCATAG GTCCATTCTC   661 TTAGCGCAAC TACAGAGAAC AGGGGCACNA ACAGGCAAAA AACGGGCACA ACCTCAATGG   721 AGTGATGCAA CCTGCCTGGA GTAAATGATG ACNCNAGGCA ATTGACCCAC GCATGTATCT   781 ATCTCATTTT CTTACACCTT CTATTACCTT CTGCTCTCTC TGATTTGNAA AAAGCTGAAA   841 AAAAANGTTG AAACCAGTTC CCTGAAANNA TTCCCNTACT TGACNAANAA GNATATAANN   901 ACNGNANGNA NTGANTGNAA TTCTGNNANT CTNNTTCNTA AACTTCNTAA ATTCTACTTT   961 TANANNNAGN CNTTTTTTTT ANNTTTAAAA NNNNNNAANN TNANNTNCNN NNNNNGNNGN 1021 CNNNNNNNNN N //
Primer: pUC-CAPbs_up_F2 (Pr0739) Region: T3 pro, hphMX (TEF pro, HygR) Sequence file: RDB18660_B0Lnb.seq
>D07536A6_B0Ln_1_pUC-CAPbs_up_F2_B07_04_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNGGCTTNNN NCTTTATGCT TCCGGCTCGT ATGTTGTGTG    61 GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC   121 GCGCAATTAA CCCTCACTAA AGGGAACAAA AGCTGGAGCT CCACCGCGGT GGCGGCCGCT   181 CTAGAACTAG TGGATCCAGT CTTGACGTGC GCAGCTCAGG GGCATGATGT GACTGTCGCC   241 CGTACATTTA GCCCATACAT CCCCATGTAT AATCATTTGC ATCCATACAT TTTGATGGCC   301 GCACGGCGCG AAGCAAAAAT TACGGCTCCT CGCTGCGGAC CTGCGAGCAG GGAAACGCTC   361 CCCTCACAGA CGCGTTGAAT TGTCCCCACG CCGCGCCCCT GTAGAGAAAT ATAAAAGGTT   421 AGGATTTGCC ACTGAGGTTC TTCTTTCATA TACTTCCTTT TAAAATCTTG CTAGGATACA   481 GTTCTCACAT CACATCCGAA CATAAACAAC CATGGGTAAA AAGCCTGAAC TCACCGCGAC   541 GTCTGTCGAG AAGTTTCTGA TCGAAAAGTT CGACAGCGTC TCCGACCTGA TGCAGCTCTC   601 GGAGGGCGAA GAATCTCGTG CTTTCAGCTT CGATGTAGGA GGGCGTGGAT ATGTCCTGCG   661 GGTAAATAGC TGCGCCGATG GTTTCTACAA AGATCGTTAT GTTTATCGGC ACTTTGCATC   721 GGCCGCGCTC CCGATTCCGG AAGTGCTTGA CATTGGGGAA TTCAGCGAGA GCCTGACCTA   781 TTGCATCTCC CGCCGTGCAC AGGGTGTCAC GTTGCAAGAC CTGCCTGAAA CCGAACTGCC   841 CGCTGTTCTG CAGCCGGNCG CGGANGCCAT GGATGCGATC GCTGCNGCCG ATCTTAGNNN   901 GANNANCGNG TTCGGNCCNT TNGGANCGCA AGGAATCGGN CAATACNCTA CNNGGCGNGA   961 NTNCATATGN NCNANTGNTG ATCCCNNNGN GNATCACTGG NAAANTGTGN TGGNNNNNNN 1021 NNN //
Primer: TDH3pro_F (Pr0431) Region: GPD pro, TOM71 5' Sequence file: RDB18660_B0Lnc.seq
>18660_18660_B0Ln_1_TDH3pro_F_C01_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNTTTTTTTA NTTTTAAAAC ACCAGAACTT AGTTTCGACG    61 GATGCGGCCG CATGGCCGAA AACTCCCTCC TGAGGTTTAT CACCAAGAAC AAAGTGGCCA   121 TCCTAGCAAC GGTCTCTGCG GGGACTGCTG CTGTGGGTGC TTATGTTTAC TACCAGCAAA   181 TTAAGCAGCA GCAACAGCAG CAATTGAAGG GTACAAAAGA CAACCGACGT CAAAGTGAGG   241 CCTTTGCTGG TCAAAATGAA GACGAGGCGG ACTTAAAGGA CGACGGCAGC GTCGTAAGTG   301 GAAGTAATAA GAGGAAGAAG AAGAAGAATA AACGCAAGAG GAACAATAAG GCAAAATCGG   361 GAGAAGGGTT TGACTATCCC TCCTTGCCCA ATGGCGAGCC TGATATTGCC CAATTGAAAG   421 GCCTATCACC CTCCCAGAGA CAAGCGTATG CCGTGCAACT GAAGAACAGA GGTAACCACT   481 TCTTCACTGC TAAGAATTTT AATGAGGCCA TCAAATACTA CCAGTATGCC ATTGAGCTGG   541 ACCCGAATGA ACCAGTGTTC TATTCCAACA TATCTGCTTG TTACATTTCT ACGGGCGACT   601 TAGAGAAGGT TATAGAGTTC ACTACAAAGG CACTTGAGAT AAAACCTGAC CATTCTAAAG   661 CCTTGCTGAG ACGTGCATCT GCCAACGAGT CACTGGGCAA CTTCACTGAT GCTATGTTTG   721 ATCTATCTGT TTTGTCNTTG AATGGTGACT TTGATGGANC CTCCATTNAA CCTATGCTGG   781 AAAGAAATTT AAATAAGCAA GCAATGAAAG TACTAAATGA AAACCTATCG AANGATGAAN   841 GCAGANGCTC GCANGTTTNG CCGNCNNACA CATCTCNNGC NNCNTTTTTT TGGGANTTTN   901 GANTCNNACT TNNAAGTTTC NAGNGNGNNN NNCANNTNCN AACTNCNATA CNNNNNACNN 961 NNNNNNNN //
Primer: paGFP-M_R1 (Pr0893) Region: GFP1-10 5', TOM71 3' Sequence file: RDB18660_B0Lnd.seq
>18660_18660_B0Ln_1_paGFP-M_R1_E01_05_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNG NNNNNNGNNN NNNGGAAAAG CATNNNNNNC CATAGGTCAG    61 AGTAGTGACA AGTGTTGGCC ATGGAACAGG TAGTTTTCCA GTAGTGCAAA TAAATTTAAG   121 TGTGAGTTTT CCGATTGTAG CATCACCTTC ACCCTCTCCT CTGACAGAAA ATTTGTGCCC   181 ATTAACATCA CCATCTAATT CAACAAGAAT TGGGACAACT CCAGTGAAAA GTTCTTCTCC   241 TTTGCTCATA CTAGTGCCAC CCATTGATCC GCCACCAGAC CCTCCGGATC CAAGCATGCC   301 TTTAGCCCTA TAACGAGCTA ATGTTAGCTC CATCTTTGCA CTTATGATTG GATCAGCCCT   361 CAAACGCTTT TGTATCTTTG CAGCTTCCGC GAAGGTAGTA GCTTGAAGTT TTTCATCCAT   421 AGTTCTTGCT AAAATTGNNN AANCNNNNNA CAATTCTATG GCCTCGTCAA TTTTTTCCAT   481 TTGTAATTTT AGTTGTGCTA ACCCGATCTT CNCTNNGTTC ANATCGTGGA TCTNATTCNC   541 ANGCTTTTGN CNNNNNCTTA ATGGCTGCNT TGAATTTCTC TTCNNCNNNN CTGGGTTGNA   601 NCNTGAGAGG ANNGTCTANC TAANATANNN GCNTTCCCNN NNNNGGNNNN NANNANNNNN 661 NNNNN //
Primer: gfp_mf (Pr0421) Region: GFP1-10 3', CYC1 ter Sequence file: RDB18660_B0Lne.seq
>18660_18660_B0Ln_1_gfp_mf_E01_13_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNAAAA CTACCNNTTC CATGGCCAAC    61 ACTTGTCACT ACTCTGACCT ATGGTGTTCA ATGCTTTTCC CGTTATCCGG ATCACATGAA   121 AAGGCATGAC TTTTTCAAGA GTGCCATGCC CGAAGGTTAT GTACAGGAAC GCACTATATC   181 TTTCAAAGAT GACGGGAAAT ACAAGACGCG TGCTGTAGTC AAGTTTGAAG GTGATACCCT   241 TGTTAATCGT ATCGAGTTAA AGGGTACTGA TTTTAAAGAA GATGGAAACA TTCTCGGACA   301 CAAACTCGAG TACAACTTTA ACTCACACAA TGTATACATC ACGGCAGACA AACAAAAGAA   361 TGGAATCAAA GCTAACTTCA CAGTTCGCCA CAACGTTGAA GATGGTTCCG TTCAACTAGC   421 AGACCATTAT CAACAAAATA CTCCAATTGG CGATGGCCCT GTCCTTTTAC CAGACAACCA   481 TTACCTGTCG ACACAAACTG TCCTTTCGAA AGATCCCAAC GAAAAGTAAG AATTCGATAT   541 CAAGCTTATC GATACCGTCG ACCTCGAGTC ATGTAATTAG TTATGTCACG CTTACATTCA   601 CGCCCTCCCC CCACATCCGC TCTAACCGAA AAGGAAGGAG TTAGACAACC TGAAGTCTAG   661 GTCCCTATTT ATTTTTTTAT AGTTATGTTA GTATTAAGAA CGTTATTTAT ATTTCAAATT   721 TTTCNTTTTT TTCTGTACNG ACGCGTGTAC GCATGTAACA TTATACTGAA AACCTTGCTT   781 GAGAANGTTT TGGGACGCTC GAANGCTTTA ATTTGCGGNN GGGCANANGA TGTCNANGCN   841 AAAAAAAATA NNAATCACGC TAACNTTTGA TTNAAATANA ACANCTACNA TATAAAAAAA   901 ACTATANNAA NTGANNAGTT NTNGAAANNN NNANNNNTTT TTTANTGNCN NNANNGNNTA 961 NTNCNTTTNN NCCNNNNNNN ANNNNNNGGN NNNNNNNNTN NNNNNNNNN //
Primer: GFP-C (Pr0304) Region: CYC1 ter, hphMX (HygR) Sequence file: RDB18660_B0Lnf.seq
>18660_18660_B0Ln_1_GFP-C_F01_16_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNACCN CNANNNATGN AATTANTTAN NNNACGCTTA    61 CATTCACGCC CTCCCCCCAC ATCCGCTCTA ACCGAAAAGG AAGGAGTTAG ACAACCTGAA   121 GTCTAGGTCC CTATTTATTT TTTTATAGTT ATGTTAGTAT TAAGAACGTT ATTTATATTT   181 CAAATTTTTC TTTTTTTTCT GTACAGACGC GTGTACGCAT GTAACATTAT ACTGAAAACC   241 TTGCTTGAGA AGGTTTTGGG ACGCTCGAAG GCTTTAATTT GCGGCCGGGC AGATGATGTC   301 GAGGCGAAAA AAAATATAAA TCACGCTAAC ATTTGATTAA AATAGAACAA CTACAATATA   361 AAAAAACTAT ACAAATGACA AGTTCTTGAA AACAAGAATC TTTTTATTGT CAGTACTGAT   421 TATTCCTTTG CCCTCGGACG AGTGCTGGGG CGTCGGTTTC CACTATCGGC GAGTACTTCT   481 ACACAGCCAT CGGTCCAGAC GGCCGCGCTT CTGCGGGCGA TTTGTGTACG CCCGACAGTC   541 CCGGCTCCGG ATCGGACGAT TGCGTCGCAT CGACCCTGCG CCCAAGCTGC ATCATCGAAA   601 TTGCCGTCAA CCAAGCTCTG ATAGAGTTGG TCAAGACCAA TGCGGAGCAT ATACGCCCGG   661 AGCCGCGGCG ATCCTGCAAG CTCCGGATGC CTCCGCTCGA AGTAGCGCGT CTGCTGCTCC   721 ATACAAGCCN ACCACGGCCT CCAGAANAAG ATGTTGGCGA CCTCGTATTG GGNATCCCCG   781 AACATCGCCT CGCTCCNGTC AATGACCGCT GTTATGCGGC CATTGTCCGT CANGACATTG   841 TTGGANCCNA AATCCGCGNG CACGANNNGN CNGANTTCNG GGNNNNCCTN NGNNCAAAGN 901 NTCNNCTCAT NNNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl