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pCI-neo MFAP4-HA WF

Expression vector of human MFAP4, mutant W235F.

Catalog number RDB18692
Resource name pCI-neo MFAP4-HA WF
Clone info. Expression vector of human Microfibril-associated glycoprotein 4 (MFAP4) mutant W235F tagged with 3×HA at C-terminus, Fig 4 of Osada, Y. et al., Biochim. Biophys. Acta Gen. Subj., 1864 (9): 129637, 2020.
Comment The MFAP4 cDNA was from the IRAK141E15 (HGX056511). Expression was confirmed by the depositor with western blotting.
Vector backbone pCI-neo (plasmid)
Size of vector backbone 5.5 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Gene/insert name human MFAP4 cDNA
Depositor|Developer Simizu, Siro |
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External Database
human MFAP4

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Osada, Y. et al., Biochim. Biophys. Acta Gen. Subj., 1864 (9): 129637, 2020).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Osada, Y. et al., Biochim. Biophys. Acta Gen. Subj., 1864 (9): 129637, 2020)。
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          RDB18692 pCI-neo MFAP4-HA WF Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Original reference

          original Osada, Y., The Fibrinogen C-terminal Domain Is Seldom C-mannosylated but Its C-mannosylation Is Important for the Secretion of Microfibril-Associated Glycoprotein 4. Biochim. Biophys. Acta. Gen. Subj. 1864 (9): 129637 (2020). PMID 32442478.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Osada, Y., The Fibrinogen C-terminal Domain Is Seldom C-mannosylated but Its C-mannosylation Is Important for the Secretion of Microfibril-Associated Glycoprotein 4. Biochim. Biophys. Acta. Gen. Subj. 1864 (9): 129637 (2020). PMID 32442478.

          2022.05.16

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