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pSFVdhfr

A clone for the IR/MAR gene amplification method. Co-transfected expression vectors with this clone can be amplified in the transfected cells.

Catalog number RDB18704
Resource name pSFVdhfr
Clone info. A clone for the IR/MAR gene amplification method. Co-transfected expression vectors with this clone can be amplified in the transfected cells. This clone contains mammalian origin of replication from the Ori beta region downstream of the Chinese hamster DHFR gene, blasticidin S and hygromycin resistance genes. Please refer Fig.1 of Shimizu, N. et al., Cancer Res., 61 (19): 6987-6990, 2001, Figs.2 and 5 of Shimizu, N. et al., Cancer Res. 63: 5281-5290, 2003, and Fig.1 of Harada, S. et al., J. Biol. Chem., 284 (36): 24320-24327, 2009.
Comment Blasticidine resistance
Vector backbone pBR322 (plasmid)
Size of vector backbone 4.4 kb
Selectable markers Ampicillin (E. coli), Hygromycin (mammalian cells)
Gene/insert name hamster Dhfr genomic
Depositor|Developer Shimizu, Noriaki |
Other clones in our bank
External Database
hamster Dhfr
            Reference sequence
              
             
            Sequence (full) RDB18704hts02.seqcheck assembled from experimentally sequenced data
            Test sheet RDB18704_B1Bhp1-2.pdf 

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Shimizu, N. et al., Cancer Res., 61 (19): 6987-6990, 2001). The BIOLOGICAL RESOURCE will not be further distributed to others without the DEPOSITOR's written consent. Any commercial use of the BIOLOGICAL RESOURCE requires prior written consent of the DEPOSITOR. Any use of the BIOLOGICAL RESOURCE for profit purposes by a non-profit organization or any use of the BIOLOGICAL RESOURCE by a profit organization requires a separate license from the DEPOSITOR prior to distribution.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Shimizu, N. et al., Cancer Res., 61 (19): 6987-6990, 2001)。利用者は、寄託者の承諾書なしに第三者への本件リソースの提供を行ってはならない。営利機関の利用者もしくは商業利用を目的とする利用者は、事前にその旨を寄託者に届出し、また提供承諾書を取得することとする。当該リソースの非営利団体の営利目的での利用および営利団体の利用については、事前に寄託者との間で、別途使用許諾に係る契約の締結が必要である。
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            RDB18704 pSFVdhfr DNA solution

            How to cite this biological resource

            Materials & Methods section:

            The pSFVdhfr was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18704).

            Reference section:

            Shimizu, N., Miura, Y., Sakamoto, Y., Tsutsui, K., Plasmids with a mammalian replication origin and a matrix attachment region initiate the event similar to gene amplification. Cancer Res. 61 (19): 6987-6990 (2001). PMID 11585721. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)

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            References

            Original, user report and related articles

            original Shimizu, N., Plasmids with a mammalian replication origin and a matrix attachment region initiate the event similar to gene amplification. Cancer Res. 61 (19): 6987-6990 (2001). PMID 11585721. [link to RRC of NBRP]

            2024.03.26

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