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pMRX-puro-EGFP-RBD11-mut

Retroviral expression vector of negative control for visualization of active Rab11: mouse Rab11-specific binding domain (RBD11) mutant tagged with EGFP.

Catalog number RDB18789
Resource name pMRX-puro-EGFP-RBD11-mut
Clone info. Retroviral expression vector of negative control for visualization of active Rab11: mouse Rab11-specific binding domain (RBD11) mutant tagged with EGFP at N-terminus. See Fig3 of Osaki, F. et al. J. Cell Sci. 134 (7) , jcs.257311, 2021.
Comment Expression was confirmed by the depositor with Immunofluorescence.
Vector backbone pMRX-puro (plasmid)
Selectable markers Ampicillin (E. coli), Puromycin (mammalian cell)
Gene/insert name mouse Rab11fip2 cDNA mouse Rab11fip4 cDNA
Depositor|Developer Fukuda, Mitsunori |
Other clones in our bank
External Database
mouse Rab11fip2 mouse Rab11fip4
        Reference sequence
          
         

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Osaki, F. et al., J. Cell Sci., 134 (7), jcs.257311, 2021).
        Additional terms and conditions:
        Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
        Ordering Please visit Information of Request for Distribution.[link] 
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        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
        Remarks Remember that you will be working with samples containing infectious virus.
        提供案内 (日本国内) [open/close]

        提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Osaki, F. et al., J. Cell Sci., 134 (7), jcs.257311, 2021)。
        付加的提供条件：
        pMRXベクターについて：利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
        提供依頼書 [Word]
        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
        備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

        Catalog # Resource name Availability Shipping form Fee (non-profit org.)
        RDB18789 pMRX-puro-EGFP-RBD11-mut Under QC test. Please contact us. DNA solution

        check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

        References and tips

        Original reference

        original Osaki, F., RBD11, a bioengineered Rab11-binding module for visualizing and analyzing endogenous Rab11. J. Cell Sci. 134 (7): jcs257311 (2021). PMID 33712449.

        Further references such as user reports and related articles (go to bottom)

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        QC test results

        check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

        References

        Original, user report and related articles

        original Osaki, F., RBD11, a bioengineered Rab11-binding module for visualizing and analyzing endogenous Rab11. J. Cell Sci. 134 (7): jcs257311 (2021). PMID 33712449.

        2023.04.06

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