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pGBD-C1-Rab30-deltaCys-N122I

Expression vector of mouse Rab30 constitutive negative mutant (N122I) lacking a C-terminal geranylgeranylation site (delta Cys) for yeast two-hybrid assay.

Catalog number RDB18891
Resource name pGBD-C1-Rab30-deltaCys-N122I
Clone info. Expression vector of mouse Rab30 constitutive negative mutant (N122I) lacking a C-terminal geranylgeranylation site (delta Cys) for yeast two-hybrid assay.
Comment Expression was confirmed by the depositor with yeast-two hybrid assay.
Vector backbone pGBD-C1 (plasmid)
Size of vector backbone 5.9 kb
Selectable markers Ampicillin (E. coli), TRP1 (yeast)
Gene/insert name mouse Rab30 cDNA
Reference of insert sequence AB232626 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
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External Database
mouse Rab30

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Fukuda, M. J. Biol. Chem., 278 (17): 15373-15380, 2003); (Osaki, F. et al., J. Cell Sci., 134 (7), jcs.257311, 2021).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Fukuda, M. J. Biol. Chem., 278 (17): 15373-15380, 2003); (Osaki, F. et al., J. Cell Sci., 134 (7), jcs.257311, 2021)。
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          RDB18891 pGBD-C1-Rab30-deltaCys-N122I Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Original reference

          original Osaki, F., RBD11, a bioengineered Rab11-binding module for visualizing and analyzing endogenous Rab11. J. Cell Sci. 134 (7): jcs257311 (2021). PMID 33712449.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Osaki, F., RBD11, a bioengineered Rab11-binding module for visualizing and analyzing endogenous Rab11. J. Cell Sci. 134 (7): jcs257311 (2021). PMID 33712449.
          reference Fukuda, M., Distinct Rab binding specificity of Rim1, Rim2, rabphilin, and Noc2. Identification of a critical determinant of Rab3A/Rab27A recognition by Rim2. J. Biol. Chem. 278 (17): 15373-15380 (2003). PMID 12578829.

          2022.05.16

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