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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pDEST/mCherry-D4

Marker of cholesterol-rich domain of the plasma membrane. Expression vector of perfringolysin theta toxin D4 with mCherry.

Catalog number RDB18944
Resource name pDEST/mCherry-D4
Clone info. Marker of cholesterol-rich domain of the plasma membrane. Expression vector of perfringolysin theta toxin D4 (domain 4 of theta toxin) of C. perfringens (ABG82518 (protein)) fused with mCherry at N-terminus.
Comment Vector, amplified mCherry was cloned into expression vector pcDNA-DEST40, generating DEST40-mCherry. Expression was confirmed by the depositor with microscopy.
Vector backbone pcDNA-DEST40 (plasmid)
Size of vector backbone 7.1 kb
Selectable markers Ampicillin
Gene/insert name Clostridium perfringens pfoA genomic DNA
Depositor|Developer Abe, Mitsuhiro |
Other clones in our bank
External Database
Clostridium perfringens pfoA
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested (Abe, M. et al., Mol. Cell. Biol., 32 (8): 1396-1407, 2020).
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks
          This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Abe, M. et al., Mol. Cell. Biol., 32 (8): 1396-1407, 2020)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考
          Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB18944 pDEST/mCherry-D4 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pDEST/mCherry-D4 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18944).

          Reference section:

          Abe, M., Makino, A., Hullin-Matsuda, F., Kamijo, K., Ohno-Iwashita, Y., Hanada, K., Mizuno, H., Miyawaki, A., Kobayashi, T., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463.

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          Featured content Visualization of Organelles (English text)
          Featured content Sphingolipid signaling pathway (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18944_B1Ctp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro, insert 5'(mCherry 5'
          Sequence file: RDB18944_B1Cta.seq check
          >D07782A2_B1Ct_1_CMV_Forward_G07_19_ABI24.ab1
              1 NNNNNNNNNN NNNNNTGNNG CNNTGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
             61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
            121 TCACTATAGG GAGACCCAAG CTGGCTAGTT AAGCTATCAG GTACCATGGT GAGCAAGGGC
            181 GAGGAGGATA ACATGGCCAT CATCAAGGAG TTCATGCGCT TCAAGGTGCA CATGGAGGGC
            241 TCCGTGAACG GCCACGAGTT CGAGATCGAG GGCGAGGGCG AGGGCCGCCC CTACGAGGGC
            301 ACCCAGACCG CCAAGCTGAA GGTGACCAAG GGTGGCCCCC TGCCCTTCGC CTGGGACATC
            361 CTGTCCCCTC AGTTCATGTA CGGCTCCAAG GCCTACGTGA AGCACCCCGC CGACATCCCC
            421 GACTACTTGA AGCTGTCCTT CCCCGAGGGC TTCAAGTGGG AGCGCGTGAT GAACTTCGAG
            481 GACGGCGGCG TGGTGACCGT GACCCAGGAC TCCTCCCTGC AGGACGGCGA GTTCATCTAC
            541 AAGGTGAAGC TGCGCGGCAC CAACTTCCCC TCCGACGGCC CCGTAATGCA GAAGAAGACC
            601 ATGGGCTGGG AGGCCTCCTC CGAGCGGATG TACCCCGAGG ACGGCGCCCT GAAGGGCGAG
            661 ATCAAGCAGA GGCTGAAGCT GAAGGACGGC GGCCACTACG ACGCTGANGT CAAGACCACC
            721 TACAAGGCCA AGAAGCCCGT GCAGCTGCCC GGCGCCTACA ACGTCAACAT CAAGTTGGAC
            781 ATCACCTCCC ACAACGAGGN CTACACCATC GTGGAACAGT ACGAACGCGC CGANGGCCGC
            841 CACTCCACCG GCGGCNTGGN CGANCTGTAC AAGAAGCTTA ANGGAAAAAT AAACTTANAT
            901 CATAGNGGAN CCTATGNTGC ACAGTTTGAA GTANCCTNNN NGNNNTTTCN TATGACNAAG
            961 NANNNAANTG AAGTTTTNNC NCNTAAAANN NGGGNNNGNN ANNNNNNNNG NNNAANNNNN
          1021 NNNNN
          //
          Primer: bGH_rev3 (Pr0885)
          Region: bGH pA, insert 3' (D4,mCherry)
          Sequence file: RDB18944_B1Ctb.seq check
          >D07782A2_B1Ct_1_bGH_rev3_C10_03_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNGNNGGG NANNAACAGN NGGCTGGCAA CTAGAAGGCA
             61 CAGTCGAGGC TGATCAGCGG GTTTAAACCT CGAGTTAATT GTAAGTAATA CTAGATCCAG
            121 GGTATAAAGT TGTTCCCCAT ATTGAAACAT TTATATTATT TGTTAATGGA ACATCATATT
            181 CACTTATAAC ATCTCTCCAC CATTCCCAAG CAAGGCCTGT ACACTCTCTT GCTTTTATTC
            241 TTATATTTCT TGCATTAGCT TCAAGAGGTA TTACTGTTGA ATAGTGAGCT GTTTTATCTT
            301 GATAATTTCC ATCCCATGTT TTATGAGTTA AAACTTCATT TCCTTCTTTG TCATATGAAA
            361 CTTCATCCCA GGCTACTTCA AACTGTGCAA CATAGGCTCC ACTATGATCT AAGTTTATTT
            421 TTCCCTTAAG CTTCTTGTAC AGCTCGTCCA TGCCGCCGGT GGAGTGGCGG CCCTCGGCGC
            481 GTTCGTACTG TTCCACGATG GTGTAGTCCT CGTTGTGGGA GGTGATGTCC AACTTGATGT
            541 TGACGTTGTA GGCGCCGGGC AGCTGCACGG GCTTCTTGGC CTTGTAGGTG GTCTTGACCT
            601 CAGCGTCGTA GTGGCCGCCG TCCTTCAGCT TCAGCCTCTG CTTGATCTCG CCCTTCAGGG
            661 CGCCGTCCTC GGGGTACATC CGCTCGGAGG AGGCCTCCCA GCCCATGGTC TTCTTCTGCA
            721 TTACGGGGCC GTCGGANGGG AAGTTGGTGC CGCGCAGCTT CACCTTGTAG ATGAACTCGC
            781 CGTCCTGCAN GGANGAGTCC TGGGTCACGG TCACCACGCC GCCGTCCTCG AAGTTCATCA
            841 CGCGCTCNCA CTTGAAGCCC TCGGGGAANG ANAGCTTCAA GTANTCNGGG ANGTCNNCGG
            901 GGNGCTTCAC GTNNNNCTNG GNNCCGTACA TGANNTNAGG GGNNAGGNNG NNCNNNGNNN
            961 ANNNNNNGGG GNNNCCCTNN NNNNNNTTNN NCTNNNCNNN CTGGGNNNCN NNNNNNNNCN
          1021 NNNNNNNNN
          //
          Primer: SV40pro_ori_F (Pr0731)
          Region: SV40 pro_ori, NeoR
          Sequence file: RDB18944_B1Ctc.seq check
          >D07782A2_B1Ct_1_SV40pro_ori_F_D10_04_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNGNN NNNTCTGCCT CTGNNCTATT CNNGAAGTAG TGAGGAGGCT
             61 TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT
            121 CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG
            181 GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG
            241 GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA
            301 AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC
            361 TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG
            421 ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG
            481 CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA
            541 CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG
            601 CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC
            661 TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG
            721 ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG
            781 GCCGGCTGGG TGTGGCGGAN CGCTATCANG ACATAGCGTT GGCTACCCGT GANATTGCTG
            841 AAGAGCTTGN CGGCGAATGG GCTGACCGCT NNCTCGNGCT TTACNGTATC GCCGCTNCNN
            901 ATTCGCAGCG CATCGCCTTC TATCGCNTTC TTGACNANNN NNTCNNNNCG GGNNTCTGGG
          961 NNNNNNCGAA ANGACNNANC NNNCNNNNNC NNNNCNNNNN NNNNN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Abe, M., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463.

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl