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pET28C-4930432K21Rik-N

Bacterial expression vector of mouse Brme1(4930432K21Rik) N-terminal portion.

Catalog number RDB19177
Resource name pET28C-4930432K21Rik-N
Clone info. Bacterial expression vector of mouse break repair meiotic recombinase recruitment factor 1 (Brme1, Aliases: 4930432K21Rik) deletion mutant (N-terminal 1 to 207 a.a.) tagged with His at N-terminus.
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone pET28c (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Kanamycin
Gene/insert name mouse Brme1 cDNA
Depositor|Developer Ishiguro, Kei-ichiro |
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External Database
mouse Brme1

          Reference sequence
            UniProtKB E9QNR8
           
          Sequence (full) RDB19177zzk01.seq provided by the depositor

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested (Takemoto, K. et al., Cell Rep., 31 (8): 107686, 2020). For use of the BIOLOGICAL RESOURCE by a for-profit organization or commercial users, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. 
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          RDB19177 pET28C-4930432K21Rik-N Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Original reference

          original Takemoto, K., Meiosis-Specific C19orf57/4930432K21Rik/BRME1 Modulates Localization of RAD51 and DMC1 to DSBs in Mouse Meiotic Recombination. Cell Rep. 31 (8): 107686 (2020). PMID 32460033.

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Takemoto, K., Meiosis-Specific C19orf57/4930432K21Rik/BRME1 Modulates Localization of RAD51 and DMC1 to DSBs in Mouse Meiotic Recombination. Cell Rep. 31 (8): 107686 (2020). PMID 32460033.

          2022.08.12

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