Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pAcGFP-PMP2 

Mammalian expression vector of human PMP2 fused with AcGFP at C-terminus.

Catalog number RDB19218
Resource name pAcGFP-PMP2 
Clone info. Mammalian expression vector of human PMP2 fused with AcGFP at C-terminus.
Comment Expression was confirmed by the depositor with microscopy.
Vector backbone pAcGFP1-Hyg-N1 (plasmid)
Size of vector backbone 5.8 kb
Selectable markers Ampicillin (E. coli), Hygromycin (mammalian cells)
Gene/insert name human PMP2 cDNA
Depositor|Developer Abe, Mitsuhiro |
Other clones in our bank
External Database
human PMP2
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested (Abe, M. et al., Cell Rep. 37: 109935 (2021)).
          Ordering
          Order form [Credit Card Payment]  [Bank Transfer Payment]
          In order to receive this biological resource, please request the Exclusive MTA to us.
          Please visit Information of Request for Distribution.[link] 
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Abe, M. et al., Cell Rep. 37: 109935 (2021))。
          提供依頼
          提供依頼書 [Word]
          本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。
          手続きの概要は、「提供申込みについて[link]」をご覧ください。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB19218 pAcGFP-PMP2  DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pAcGFP-PMP2  was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19218).

          Reference section:

          Abe, M., Makino, A., Murate, M., Hullin-Matsuda, F., Yanagawa, M., Sako, Y., Kobayashi, T., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

          Tips

          Featured content

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB19218_B1Grp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: Venus-N_R (Pr0341)
          Region: AcGFP1 5', insert, CMV pro
          Sequence file: RDB19218_B1Gra.seq check
          >19218_19218_B1Gr_Venus-N_R_H02_23_ABI24.ab1
              1 NNNNNNNNNA NNNNNNNGGG CACGATGCCG GTGAACAGCT CGGCGCCCTT GCTCACCATG
             61 ACCGGTGGAT CCCGGGCCCG CGGTACCGTC GACTGCAGAA TTCGAAGCTT GACCTTCTCA
            121 TAGATTCTGG TGCACACCAC GCCCTTCATT TTACATTCCG CTACCATTTT CCCATTCACT
            181 AGCTTTCTCT TTATGGTTGT CTCTTTGCCA TCCCATCTCT GCACTTGATT CAGTGATCCT
            241 CTCTGCAGGG TTACGATGCT CTTGGTCTTT CTATTGTCAG CTGTGGTTTC TTCAAATTCC
            301 TGGCCTAGCT TGAAGGAGAT TTCTGTATTT TTAAAGGTAC TTTCAGTTCG TATAGTTATA
            361 ATATCTCCTT TCTTGCTGAT GATCACAGTG GGTTTGGCCA AATTTCCCAG TTTTCTGGTG
            421 GCTAACCCCA CACCCAGAGC TTTCATGTAA TCGTCAAAGT TCTCACTAGA GACAAGTTTC
            481 CAGGTGCCCA GGAATTTGTT GCTCATGCTA GCGGATCTGA CGGTTCACTA AACCAGCTCT
            541 GCTTATATAG ACCTCCCACC GTACACGCCT ACCGCCCATT TGCGTCAATG GGGCGGANTT
            601 GTTACGACAT NNNGNAAAGT CCCGTTGANT TTGGTGNNNA AACAAACTCC NNTTGNNGTC
          661 NATGGGGNGG ANNNTTNNAA ATCCCNNNNN NNCNANNNNN NNNNN
          //
          Primer: pAxEF R2 (Pr0059)
          Region: SV40 pA, AcGFP1 3'
          Sequence file: RDB19218_B1Grb.seq check
          >19218_19218_B1Gr_pAxEFF1_F02_17_ABI24.ab1
              1 NNNNNNNNNN NNNNTGTGNN GCTATTGCTT TATTTGTAAC CATTATAAGC TGCAATAAAC
             61 AAGTTAACAA CAACAATTGC ATTCATTTTA TGTTTCAGGT TCAGGGGGAG GTGTGGGAGG
            121 TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTATGGC TGATTATGAT CTAGAGTCGC
            181 GGCCGCTCAC TTGTACAGCT CATCCATGCC GTGGGTGATG GCGGCGGCGG TCACGAAGCC
            241 GAAGTAGATC ATGTGATCGC GCTTCTCGTT GGGGTCCTTG GACAGGGCGC TCTGGGTGGA
            301 CAGGTAGTGG TTATCGGGCA GCAGCACAGG GCCATCGCCG ATGGGGGTAT TCTGCTGGTA
            361 GTGGTCGGCC AGCTGCACGC TGCCATCCTC GATGTTGTGG CGGATCTTGA AGTTCACCTT
            421 GATGCCATTC TTGGCCTTGT CGGTCATGAT GTACACATTG TGGGCGTTGT AGTTGTACTC
            481 CATCTTATTG CCCAGGATGT TGCCATCCTC CTTGAAATCG GTGCCGGTCA GCTCGATGCG
            541 ATTCACCAGG GTATCGCCCT CGAACTTCAC CTCGGCGCGC GACTTGTAGT TGCCGTCNTC
            601 CTCNAAGAAN NNGGTGCGCT CCTGGANGTA GCCCTCNNNC NTGGCGCTCT TGAANAANTC
          661 GTGCTGCNTN NTGTGATCNG GNNANCGNGA GAAGCACTGC NCGCNNNNNN NNNN
          //
          Primer: SV40pro_ori_F (Pr0731)
          Region: SV40 pro_ori, HygR
          Sequence file: RDB19218_B1Grc.seq check
          >19218_19218_B1Gr_SV40pro_ori_F_G02_20_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNC CTCTGNNCTA TTCNNNNNNT AGTGAGGAGG CTTTTTTGGA
             61 GGCCTAGGCT TTTGCAAAGA TCGATATGAA AAAGCCTGAA CTCACCGCGA CGTCTGTCGA
            121 GAAGTTTCTG ATCGAAAAGT TCGACAGCGT CTCCGACCTG ATGCAGCTCT CGGAGGGCGA
            181 AGAATCTCGT GCTTTCAGCT TCGATGTAGG AGGGCGTGGA TATGTCCTGC GGGTAAATAG
            241 CTGCGCCGAT GGTTTCTACA AAGATCGTTA TGTTTATCGG CACTTTGCAT CGGCCGCGCT
            301 CCCGATTCCG GAAGTGCTTG ACATTGGGGA ATTCAGCGAG AGCCTGACCT ATTGCATCTC
            361 CCGCCGTGCA CAGGGTGTCA CGTTGCAAGA CCTGCCTGAA ACCGAACTGC CCGCTGTTCT
            421 GCAGCCGGTC GCGGAGGCCA TGGATGCGAT CGCTGCGGCC GATCTTAGCC AGACGAGCGG
            481 GTTCGGCCCA TTCGGACCGC AAGGAATCGG TCAATACACT ACATGGCGTG ATTTCATATG
            541 CGCGATTGCT GATCCCCATG TGTATCACTG GCAAACTGTG ATGGACGACA CCGTCAGTGC
            601 GTCCGTCGCG CANNCTCTCG ATGANCTGAN GCTTTGGGNC GAGGANTGNC CNNANNTNCG
            661 GCACCTCGNG NNNGCNGANT TCNNNTNCNA CNNTGTCCTG ANNNANNNTN NNCNNNNNNN
          721 NNN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Abe, M., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. [link to RRC of NBRP]

          2023.12.13

          GNP_filter3_RDBDEP_html_231213.pl