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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pAID1.2-CMV-NmScarlet-mAID

Expresson vector to construct mScarlet-mAID tagged target gene and OsTIR1 (WT) simultaneously by CMV promoter for the super-sensitive AID system.

Catalog number RDB19226
Resource name pAID1.2-CMV-NmScarlet-mAID
Clone info. Expresson vector to construct mScarlet-mAID tagged target gene and OsTIR1 (WT) simultaneously under control of the CMV promoter for the super-sensitive auxin-inducible degron (AID) system. See Fig.1 of Nishimura, K. et al. Nucleic Acids Res. 48 (18): e108, 2020.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kanamycin (E. coli), G418 (mammalian cell)
Gene/insert name oriza sativa OsTIR1 cDNA
Depositor|Developer Fukagawa, Tatsuo |
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External Database
oriza sativa OsTIR1

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Nishimura, K. et al. Nucleic Acids Res. 48 (18): e108, 2020).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Nishimura, K. et al. Nucleic Acids Res. 48 (18): e108, 2020)。
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          RDB19226 pAID1.2-CMV-NmScarlet-mAID DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pAID1.2-CMV-NmScarlet-mAID was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19226).

          Reference section:

          Nishimura, K., Yamada, R., Hagihara, S., Iwasaki, R., Uchida, N., Kamura, T., Takahashi, K., Torii, K.U., Fukagawa, T., A super-sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair. Nucleic Acids Res. 48 (18): e108 (2020). PMID 32941625. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


          Tips

          Featured content

          Featured content Auxin Inducible Degron (AID) System (English text)
          Featured content Auxin Inducible Degron (AID) System (Japanese text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB19226_B1H2p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro, OsTIR1 5'
          Sequence file: RDB19226_B1H2a.seq check
          >D08293A1_B1H2_3_CMV_Forward_A01_01_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNTNN NNNNNNATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
             61 TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCCGCTAGCG CTGCCACCAT GACATACTTT
            121 CCTGAAGAGG TCGTCGAACA CATTTTTAGC TTCCTGCCTG CACAGAGAGA TAGAAACACA
            181 GTGAGCCTGG TCTGCAAAGT GTGGTACGAG ATCGAACGCC TGAGCCGGAG AGGAGTGTTC
            241 GTCGGCAACT GCTATGCTGT GAGAGCAGGC AGGGTCGCCG CTAGGTTTCC AAATGTGCGC
            301 GCACTGACCG TCAAGGGGAA ACCCCACTTC GCCGACTTTA ACCTGGTGCC CCCTGATTGG
            361 GGAGGATACG CCGGCCCTTG GATCGAGGCA GCCGCTCGCG GCTGTCATGG ACTGGAGGAA
            421 CTGCGCATGA AGCGAATGGT GGTCTCTGAC GAAAGTCTGG AGCTGCTGGC TCGGAGCTTC
            481 CCTAGGTTTC GCGCACTGGT GCTGATTTCT TGCGAAGGCT TCAGCACCGA TGGACTGGCA
            541 GCCGTGGCCT CCCACTGTAA GCTGCTGCGG GAGCTGGACC TCCAGGAGAA TGAAGTGGAG
            601 GATAGAGGCC CCAGATGGCT GTCTTGCTTC CCAGACTCAT GTACCAGCCT GGTGTCCCTG
            661 AACTTTGCCT GCATCAAAGG CGAAGTGAAT GCTGGGTCCC TGGAGCGGCT GGTCTCAAGA
            721 AGCCCCAACC TGAGGTCTCT GCGGCTGANC CGGANCGTGA GCGTGGACAC TCTGGCTAAG
            781 ANTCTGCTGA NAACCCCTAA CCTGGANGAN CTGGGAACCG GCAATCTGAC AGANGANTTC
            841 CNGACAGANT CCTACTTTAA ACTGACTTCT GNCCTGGANA ANTGTAAAAT GNTGANGANT
            901 CTGTCNNNTT CTGGGATGCT TNACCCNNNN GNNNGANCTT TATCNNCCCN NNGNGNGCNN
          961 AGCTGANNNN NNNGNNNNNN ANNNNTGCAN NNNNCNNNNN NNNNN
          //
          Primer: SV40polyA_R2 (Pr0623)
          Region: insert (mini AID, mScarlet 3')
          Sequence file: RDB19226_B1H2b.seq check
          >D08293A1_B1H2_3_SV40polyA_R2_D01_10_ABI24.ab1
              1 NNNNNNNNNN NNNNNCNNNN NNNGTTANNN ACNACAATTG GATATCGGCA CCCGCTCCNG
             61 CGCCTGCACC AGCTCCTTTA TACATCCTCA AATCGATTTT CCTCAAGTAC GGTGCTCCGT
            121 CCATTGATAC CTTCACGAAC GCCGCCGCCT CCGGGCCACC GCTTGATTTT TGGCAGGAAA
            181 CCATCACGTT CTTCCGGTAT GATCTCACCG GTGGCCATCC CACAACTTGT GCCTTGGCCG
            241 GAGGTTTGGC TGGATCTTTA GGACAAGCAC TCTTCTCCTT CTTGTACAGC TCGTCCATGC
            301 CGCCGGTGGA GTGGCGGCCC TCGGAGCGTT CGTACTGTTC CACCACGGTG TAGTCCTCGT
            361 TGTGGGAGGT GATGTCCAAC TTGCGGTCGA CGTTGTAGGC GCCGGGCATC TGCACGGGCT
            421 TCTTGGCCTT GTAGGTGGTC TTGAAGTCCG CCAGGTAGCG GCCGCCGTCC TTCAGGCGCA
            481 GGGCCATCTT AATGTCGCCC TTCAGCACGC CGTCCTCGGG GTACAACCGC TCGGTGGACG
            541 CTTCCCAGCC CATTGTCTTC TTCTGCATTA CGGGGCCGTC AGGAGGGAAG TTGGTGCCGC
            601 GGAGCTTCAC CTTGTAGATC AGGGTGCCGT CCTCCAGGGA GGTGTCCTGG GTCACGGTCA
            661 CGGCGCCGCC GTCCTCGAAG TTCATCACGC GCTCCCACTT GAAGCCCTCG GGGAANGANT
            721 GCTTATAGTA GTCGGGGANG TCGGCGGGGT GCTTGATGAA GGCCCTGGAG CCGTACATGA
            781 ACTGANGGGA NAGGATGTCN CAGGAGAANG GCANGGGGCC ACCCTTGGTC ACCTTCAGCT
            841 TGGCGGTCTG GGTGNCCTCN NAGGGGCGGC CCTCGCCCTC GCCCNCGATC NCNAANTCNN
            901 GGCCGTNCAT GGANCCCNNC NTGTGCACCT TGNNCCGCAT GAACTCCNNG ATCACTGNNN
          961 CNCCCTTGNN CANCANNNNA TCGCACATNN NNNNNNNN
          //
          Primer: IRES-R (Pr0037)
          Region: insert (bsr, T2A, OsTIR1 3')
          Sequence file: RDB19226_B1H2c.seq check
          >D08293A1_B1H2_3_IRES-R_A09_03_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GNNGNNNNNN NNNNNANGGG
             61 CGGATCCCGG GGNTCTCTTG AAATGATCGT TCCACCTCAA AGGGGCTTCA TGATGGTCCC
            121 CATAATTTTT GGCAGAGGGA AAAAGATCNN NNNTTTCGGG TATATTTGAG TGGAATGAGT
            181 TCTTCAATCG TAGTTTTGAC TAACTTGCCA TTCATTTCTA TTAACACAAA ACAATCTGGT
            241 GCATAGTCTG AAATCAACTC CCTACACATA CCACAAGGAC TTACCACTCG AATACTTCTA
            301 TCTACTTCGT CAGAATAAGG GTGTCTAACA GCTACAATCG TGTCAAAATC CTTTTGTCCA
            361 TTCGAAACTG CACTACCAAT CGCAATGGCT TCTGCACAaA CaGTTACTCG TCCTATATAC
            421 GCTTCAATAT GTACTGCCGA AATGATTTCT CCTGTTTTCG TACGAATTGC CGCTCCCACA
            481 TGATGTTTAT TATCCTCATA AAGCATTGTA ATCTTCTCTG TCGCTACTTC TACTAATTCT
            541 AGATCTTGTT GAGAAATGTT AAATGTTTTC ATGGGGCCGG GGTTCTCCTC CACGTCGCCG
            601 CAGGTCAGCA GGCTGCCGCG GCCCTCCAGA ATCTTCACAA AGTTGGGAGC ATCNTCTCTT
            661 GCCCCTGCGG TGGTCCGAAT ACACATACAG TTTCTCCNNC TTTGGCAGGT CNCCATGATT
            721 TTCCTCNNNT TCGTTAGACC NNNNCCGCTC ATTAATGANT TCCACGTTCA NNATAGGCAT
            781 TTTACTAGCC AGGANCNTGA CAGCCCTTCA NAGTCACATT GCAACTANAC ATCCANNNGC
            841 TCCTCATNGT CTNCNNANNT GGCNNANNNT TCCCNNNNNA NCGGNCNNTC CCNNAAANNG
            901 NNTGNNNCCG NANTTNCNNG TTTTNCGCAN ATNCTTACNC CCCNTTCATC NNGNNNNTNN
            961 NGNCCTTANN NNNANNNNNN NNCNAATGCA ANNNTNANCA TCTCCNNNNN GNNTTANNAN
          1021 ANTTGCCNAA NGNNNNTGNN NNNNTTTNNN NNNNNNN
          //
          Primer: IRES-F (Pr0036)
          Region: IRES2, mScarlet 5'
          Sequence file: RDB19226_B1H2d.seq check
          >D08293A1_B1H2_3_IRES-F_C01_07_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNTNN NNNNNANGTT NNNNNNNCGT CTAGGCCCCC CGAACCNCGG
             61 GGACGTGGTT TTCCTTTGAA AAACACGATG ATAATATGGC CACAACCATG TGCGATAACA
            121 TGGTGAGCAA GGGCGAGGCA GTGATCAAGG AGTTCATGCG GTTCAAGGTG CACATGGAGG
            181 GCTCCATGAA CGGCCACGAG TTCGAGATCG AGGGCGAGGG CGAGGGCCGC CCCTACGAGG
            241 GCACCCAGAC CGCCAAGCTG AAGGTGACCA AGGGTGGCCC CCTGCCCTTC TCCTGGGACA
            301 TCCTGTCCCC TCAGTTCATG TACGGCTCCA GGGCCTTCAT CAAGCACCCC GCCGACATCC
            361 CCGACTACTA TAAGCAGTCC TTCCCCGAGG GCTTCAAGTG GGAGCGCGTG ATGAACTTCG
            421 AGGACGGCGG CGCCGTGACC GTGACCCAGG ACACCTCCCT GGAGGACGGC ACCCTGATCT
            481 ACAAGGTGAA GCTCCGCGGC ACCAACTTCC CTCCTGACGG CCCCGTAATG CAGAAGAAGA
            541 CAATGGGCTG GGAAGCGTCC ACCGAGCGGT TGTACCCCGA GGACGGCGTG CTGAAGGGCG
            601 ACATTAAGAT GGCCCTGCGC CTGAAGGACG GCGGCCGCTA CCTGGCGGAC TTCAAGACCA
            661 CCTACAAGGC CAAGAAGCCC GTGCAGATGC CCGGCGCCTA CAACGTCGAC CGCAAGTTGG
            721 ACATCACCTC CCACAACGAG GACTACACCG TGGTGGAACA GTACGAACGC TCCGANGGCC
            781 GCCACTCCAC CGGCGGCNTG GACGANCTGT ACNAGAANGA NAAGANTGCT TGTCCTAAAG
            841 ATCCAGCCNA ACCTCCGGCC AANGNACAAG TNGTGGGATG GNCNCCGGTG ANANCATACC
            901 GGAANANNGN GATGGTTNCN NGNNNAAAAT CAAGCGGNNG NCCNGANGNN NNGGNNTTCN
          961 NGNNNNNNTC NNNNNNNNNN
          //
          Primer: SV40pro_ori_F (Pr0731)
          Region: SV40 pro_ori, NeoR_KanR
          Sequence file: RDB19226_B1H2e.seq check
          >D08293A1_B1H2_3_SV40pro_ori_F_E01_13_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNCTCG GCCTCTGNNC TATTCCNGAA GTAGTGAGGA
             61 GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA GATCGATCAA GAGACAGGAT GAGGATCGTT
            121 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT
            181 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT
            241 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA
            301 ACTGCAAGAC GAGGNNNCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC
            361 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG
            421 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC
            481 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA
            541 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA
            601 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC
            661 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA
            721 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ANCGCTATCA
            781 NGACATAGCG TTGGCTACCC GTGANATTGC TGAAGAGCTT GGCGGCNAAT GGGCTGACCG
            841 CTTCCTCGTG CTTTACNGGN ATCGCCGCTC CCGATTCGCA NCGCATCGCC TTCTATCGNN
            901 NNNTTGACNA NTNCTTCTGA NNNGNANTCT GGGGNNNGAA ATGANCGANC NANCNACNNC
          961 NNACCNNNCN TCACNANNNT TNNATTNCNN CNGNCNNNNN NNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Nishimura, K., A super-sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair. Nucleic Acids Res. 48 (18): e108 (2020). PMID 32941625. [link to RRC of NBRP]

          2024.03.26

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