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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pAIDFA-EF1a-NmScarlet-mAID

Expresson vector to construct mScarlet-mAID tagged target gene and OsTIR1 mutant (F74A) simultaneously by EF1 alpha promoter for the super-sensitive AID system.

Catalog number RDB19229
Resource name pAIDFA-EF1a-NmScarlet-mAID
Clone info. Expresson vector to construct mScarlet-mAID tagged target gene and OsTIR1 mutant (F74A) simultaneously under the control of EF1 alpha promoter for the super-sensitive auxin-inducible degron (AID) system. See Fig.1 of Nishimura, K. et al. Nucleic Acids Res. 48 (18): e108, 2020.
Vector backbone pBlueScript SK- (plasmid)
Selectable markers Ampicillin
Gene/insert name oriza sativa OsTIR1 cDNA
Depositor|Developer Fukagawa, Tatsuo |
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External Database
oriza sativa OsTIR1

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Nishimura, K. et al. Nucleic Acids Res. 48 (18): e108, 2020).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Nishimura, K. et al. Nucleic Acids Res. 48 (18): e108, 2020)。
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          RDB19229 pAIDFA-EF1a-NmScarlet-mAID DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pAIDFA-EF1a-NmScarlet-mAID was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19229).

          Reference section:

          Nishimura, K., Yamada, R., Hagihara, S., Iwasaki, R., Uchida, N., Kamura, T., Takahashi, K., Torii, K.U., Fukagawa, T., A super-sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair. Nucleic Acids Res. 48 (18): e108 (2020). PMID 32941625. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


          Tips

          Featured content

          Featured content Auxin Inducible Degron (AID) System (English text)
          Featured content Auxin Inducible Degron (AID) System (Japanese text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB19229_B1H2p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EF1a-C_F
          Sequence file: RDB19229_B1H2a.seq check
          >D08293A4_B1H2_3_EF1a-C_F_A07_01_ABI24.ab1
              1 NNNNNNNNNN NNNNNTTNNN ANTTTGGNNC TTGGTTNNTT CTCNAGCCTC NGACAGTGGT
             61 TCNAAGTTTT TTTCTTCCNT TTCAGGTGTC GTGAATTGCG GAAGGCCGTC AAGGCCACGT
            121 GTCTTGTCCA GAGGCGCTGC CACCATGACA TACTTTCCTG AAGAGGTCGT CGAACACATT
            181 TTTAGCTTCC TGCCTGCACA GAGAGATAGA AACACAGTGA GCCTGGTCTG CAAAGTGTGG
            241 TACGAGATCG AACGCCTGAG CCGGAGAGGA GTGTTCGTCG GCAACTGCTA TGCTGTGAGA
            301 GCAGGCAGGG TCGCCGCTAG GTTTCCAAAT GTGCGCGCAC TGACCGTCAA GGGGAAACCC
            361 CACGCCGCCG ACTTTAACCT GGTGCCCCCT GATTGGGGAG GATACGCCGG CCCTTGGATC
            421 GAGGCAGCCG CTCGCGGCTG TCATGGACTG GAGGAACTGC GCATGAAGCG AATGGTGGTC
            481 TCTGACGAAA GTCTGGAGCT GCTGGCTCGG AGCTTCCCTA GGTTTCGCGC ACTGGTGCTG
            541 ATTTCTTGCG AAGGCTTCAG CACCGATGGA CTGGCAGCCG TGGCCTCCCA CTGTAAGCTG
            601 CTGCGGGAGC TGGACCTCCA GGAGAATGAA GTGGAGGATA GAGGCCCCAG ATGGCTGTCT
            661 TGCTTCCCAG ACTCATGTAC CAGCCTGGTG TCCCTGAACT TTGCCTGCAT CAAAGGCGAA
            721 GTGAATGCTG GGTCCCTGGA NCGGCTGGTC TCAAGAAGCC CCAACCTGAN GTCTCTGCGG
            781 CTGANNCGGA NCGTGAGCGT GGACACTCTG GCTAAGATTC TGCTGANANN CCCTAANCTG
            841 NAGGATCTGG GAANCGGCAA TCTGANAGAN NANTTCCAGA CAGAATCCTA CTTTAAACTG
            901 ANTTCTGNCN NNGANAANTG TAAAATGCTG ANNANNCTGT CNNNNNTCTG GNNNGCTTCN
          961 CCCGNGNNCN TGANCTTTNT CTACCCNNNN NNNGNNNAGC NNNNNNNNNN
          //
          Primer: IRES-R
          Sequence file: RDB19229_B1H2b.seq check
          >D08293A4_B1H2_3_IRES-R_B07_04_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNGGGG GGGGNNNNNG NNANAGGGGC
             61 GGNTCCCTCA AGGGGCTTCA TGATGTCCCC ATAATTTTTG GCAGAGGGAA AAAGATCCTT
            121 AATTTCGGGT ATATTTGAGT GGAATGAGTT CTTCAATCGT AGTTTTGACT AACTTGCCAT
            181 TCATTTCTAT TAACACAAAA CAATCTGGTG CATAGTCTGA AATCAACTCC CTACACATAC
            241 CACAAGGACT TACCACTCGA ATACTTCTAT CTACTTCGTC AGAATAAGGG TGTCTAACAG
            301 CTACAATCGT GTCAAAATCC TTTTGTCCAT TCGAAACTGC ACTACCAATC GCAATGGCTT
            361 CTGCACAAAC AGTTACTCGT CCTATATACG CTTCAATATG TACTGCCGAA ATGATTTCTC
            421 CTGTTTTCGT ACGAATTGCC GCTCCCACAT GATGTTTATT ATCCTCATAA AGCATTGTAA
            481 TCTTCTCTGT CGCTACTTCT ACTAATTCTA GATCTTGTTG AGAAATGTTA AATGTTTTCA
            541 TGGGGCCGGG GTTCTCCTCC ACGTCGCCGC AGGTCAGCAG GCTGCCGCGG CCCTCCAGAA
            601 TCTTCACAAA GTTGGGAGCA TCATCTCTTG CCCCTGCGGT GGTCCGANNN NNNNACAGTT
            661 TCTCCACCTT TGNNNNNNCG CCATGATTTT CCTCNNNTTC GTTAGACCCG TCCCGCTCAT
            721 TAATGACTTC CACGTTCAGC ATANGCATTT TACTANNCAG GACCTGACAG CCCTTCANAG
            781 TCACATTGCA ACTAGACATC CACAGGCTCC TCATTGTCTC GTATCTGGNA AAAGTTTCCC
            841 AGCAGANCGG CATCCCCGAA AGGGCTGTCC CGGATTTCCN NTTTTCGCAN ANTCNTACNC
            901 CCGTTCATCA CGNGCATCAT GCCCNTATNN GANTNTCCGG NAAANGNNAT GNTCANCATC
            961 TNNNNNTGCT NNNCANNNTT GNNNANGTNC NNGANNNNNT TTNNNNGNNN NCNNNNNNGA
          1021 AANGNNNANN NTCNNNNNNN NCCNTNNNNN NNNNN
          //
          Primer: IRES-F
          Sequence file: RDB19229_B1H2c.seq check
          >D08293A4_B1H2_3_IRES-F_C07_07_ABI24.ab1
              1 NNNNNNNNNN NNNNNTTNNG TCGANGTNNN NNNNNNTCTA GGCCCCCCGA ACCNCGGGGA
             61 CGTGGTTTTC CTTTGAAAAA CACGATGATA ATATGGCCAC AACCATGTGC GATAACATGG
            121 TGAGCAAGGG CGAGGCAGTG ATCAAGGAGT TCATGCGGTT CAAGGTGCAC ATGGAGGGCT
            181 CCATGAACGG CCACGAGTTC GAGATCGAGG GCGAGGGCGA GGGCCGCCCC TACGAGGGCA
            241 CCCAGACCGC CAAGCTGAAG GTGACCAAGG GTGGCCCCCT GCCCTTCTCC TGGGACATCC
            301 TGTCCCCTCA GTTCATGTAC GGCTCCAGGG CCTTCACCAA GCACCCCGCC GACATCCCCG
            361 ACTACTATAA GCAGTCCTTC CCCGAGGGCT TCAAGTGGGA GCGCGTGATG AACTTCGAGG
            421 ACGGCGGCGC CGTGACCGTG ACCCAGGACA CCTCCCTGGA GGACGGCACC CTGATCTACA
            481 AGGTGAAGCT CCGCGGCACC AACTTCCCTC CTGACGGCCC CGTAATGCAG AAGAAGACAA
            541 TGGGCTGGGA AGCGTCCACC GAGCGGTTGT ACCCCGAGGA CGGCGTGCTG AAGGGCGACA
            601 TTAAGATGGC CCTGCGCCTG AAGGACGGCG GCCGCTACCT GGCGGACTTC AAGACCACCT
            661 ACAAGGCCAA GAAGCCCGTG CAGATGCCCG GCGCCTACAA CGTCGACCGC AAGTTGGACA
            721 TCACCTCCCA CAACGAGGAC TACACCGTGG TGGAACAGTA CGAACGCTCC GANGGCCGCC
            781 ACTCCACCGG CGGCATGGNC GANCTGTACA AGAANGAGAA GANTGCTTGT CCTAAAGATC
            841 CAGCCNAACC TCCGGCCNAG GNACAAGTTG TGGGANGGCC NCCGGNGANA TCATACCGGA
          901 ANAACGTGNT GGTTTNCTGC CAAAAATCNN NCGGNGGCCC NNANNNNNNN NNN
          //
          Primer: SV40polyA_R2
          Sequence file: RDB19229_B1H2d.seq check
          >D08293A4_B1H2_3_SV40polyA_R2_D07_10_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNAN GTTAANNNNN NNNATTGGAT ATCGGCACCC
             61 GCTCCAGCGC CTGCACCAGC TCCTTTATAC ATCCTCAAAT CGATTTTCCT CAAGTACGGT
            121 GCTCCGTCCA TTGATACCTT CACGAACGCC GCCGCCTCCG GGCCACCGCT TGATTTTTGG
            181 CAGGAAACCA TCACGTTCTT CCGGTATGAT CTCACCGGTG GCCATCCCAC AACTTGTGCC
            241 TTGGCCGGAG GTTTGGCTGG ATCTTTAGGA CAAGCACTCT TCTCCTTCTT GTACAGCTCG
            301 TCCATGCCGC CGGTGGAGTG GCGGCCCTCG GAGCGTTCGT ACTGTTCCAC CACGGTGTAG
            361 TCCTCGTTGT GGGAGGTGAT GTCCAACTTG CGGTCGACGT TGTAGGCGCC GGGCATCTGC
            421 ACGGGCTTCT TGGCCTTGTA GGTGGTCTTG AAGTCCGCCA GGTAGCGGCC GCCGTCCTTC
            481 AGGCGCAGGG CCATCTTAAT GTCGCCCTTC AGCACGCCGT CCTCGGGGTA CAACCGCTCG
            541 GTGGACGCTT CCCAGCCCAT TGTCTTCTTC TGCATTACGG GGCCGTCAGG AGGGAAGTTG
            601 GTGCCGCGGA GCTTCACCTT GTAGATCAGG GTGCCGTCCT CCAGGGAGGT GTCCTGGGTC
            661 ACGGTCACGG CGCCGCCGTC CTCGAAGTTC ATCNCGCGCT CCCACTTGAA GCCCTCGGGG
            721 AANGACTGCT TATAGTAGTC GGGGATGTCG GCGGGGTGCT TGGTGAANGN CCTGGAGCCG
            781 TACATGAACT GAGGGGANAG GATGTCCCAN GANAAGGGCA GGGGGCCACC CTTGGTCACC
            841 TTCAGCTTGG CGGNCTGGGT GNCCTCGTAN GGGCGGCCCT CNCCCTCGCC CTCGATCTCN
            901 AACTCGNGGC CGTTCATGGA GCCCTNCATG NGCACCNTNG NACCGCATGN ACTNCTNNAT
            961 CACTGNCNTN GCCCNNTNNN TCACCATGNT ATCNNNNNNN GNNNGTNNNC NNNNNNANNN
          1021 NCNNGNTTTT CNANNNNAAN NNNNNNNNCN CNNNNNNNNN N
          //
          Primer: SV40pro_R
          Sequence file: RDB19229_B1H2e.seq check
          >D08293A4_B1H2_3_SV40pro_R_E07_13_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNATGGGC NNNNNGGGCG
             61 GANTTNNGGN CGNGNNGGGC GGANTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT
            121 GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGTTA GGCCAGAGAA ATGTTCTGGC
            181 ACCTGCACTT GCACTGGGGA CAGCCTATTT TGCTAGTTTG TTTTGTTTCG TTTTGTTTTG
            241 ATGGAGAGCG TATGTTAGTA CTATCGATTC ACACAAAAAA CCAACACACA GATGTAATGA
            301 AAATAAAGAT ATTTTATTGC GGCCTGTCCA ATACCTCCCG TACCTTAATA TTACTTACTT
            361 ATCCTTGAGA GACGTACTAG TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGACGA
            421 GTATGAGCAT CCAACATTTT CGTGTCGCAC TCATTCCCTT CTTTGCGGCA TTTTGCTTGC
            481 CTGTTTTTGC ACACCCCGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAACTGGGTG
            541 CAAGAGTGGG CTATATCGAA CTGGATCTCA ATAGCGGCAA GATCCTTGAG TCTTTCCGCC
            601 CCGAAGAACG ATTCCCGATG ATGAGCACTT TCNAAGTACT GCTATGTGGC GCGGTGTTGT
            661 CCCGTATAGA CGCCGGGCAA GAGCAGCTTG GTCGCCGTAT ACACTACTCA CAAAACGACN
            721 NTGNNTGANT ACTCGNNCGG TCACGGAAAA GCATCTTACN GGATGGCATN NNNNGGTAAG
            781 AGAANNTGTG TANTGCTGNC CATTTACCAT GNNCGNNNAN CNCCCGCGNN NNNNTTACTT
            841 TCTGACNNCG ATCGNNANGN CNNNNNNGNA NNNNACNNGC NNTTNNNNNN NTANNTNTGG
          901 GNNGATNNNT GNGACNNGGN NNTNNNNCNN NNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Nishimura, K., A super-sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair. Nucleic Acids Res. 48 (18): e108 (2020). PMID 32941625. [link to RRC of NBRP]

          2024.03.26

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