Expression vector of Cas9 protein and sgRNA to linearize pAID-CMV plasmids in vivo.
|
Other clones in our bank
External Database Reference sequence |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Nishimura, K. et al. Nucleic Acids Res. 48 (18): e108, 2020). |
---|---|
Ordering | Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word] |
Remarks | The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations. |
提供条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Nishimura, K. et al. Nucleic Acids Res. 48 (18): e108, 2020)。 |
---|---|
提供依頼 | 手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 専用MTA(CRISPR/Cas9内包遺伝子材料専用 非営利学術目的)をお使いください [Word] |
備考 | 本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
---|---|---|---|
RDB19230 | pAID1.2-CMV-linearizing in pX330 | DNA solution |
Materials & Methods section:
The pAID1.2-CMV-linearizing in pX330 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19230). |
Reference section:
Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Auxin Inducible Degron (AID) System (English text) |
---|---|
Featured content | Auxin Inducible Degron (AID) System (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB19230_B1H2p1-1.pdf |
---|
Nucleotide sequence of a portion of this resource (if available).
Primer: U6pro_F3 (Pr0648) Region: U6 pro,sgRNA for pAID-CMV,CMV en,chicken β-actin pro Sequence file: RDB19230_B1H2a.seq |
---|
>D08293A5_B1H2_2_U6pro_F3_E01_13_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNGG NNTATNNTAT GCTTACCGTA ACTTGNAAGT ATTTCGATTT 61 CTTGGCTTTA TATATCTTGT GGAAAGGACG AAACACCGCC AAGTTCGGGT GAAGGCCCGT 121 TTAAGAGCTA TGCTGGAAAC AGCATAGCAA GTTTAAATAA GGCTAGTCCG TTATCAACTT 181 GAAAAAGTGG CACCGAGTCG GTGCTTTTTT GTTTTAGAGC TAGAAATAGC AAGTTAAAAT 241 AAGGCTAGTC CGTTTTTAGC GCGTGCGCCA ATTCTGCAGA CAAATGGCTC TAGAGGTACC 301 CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT 361 GACGTCAATA GTAACGCCAA TAGGGACTTT CCATTGACGT CAATGGGTGG AGTATTTACG 421 GTAAACTGCC CACTTGGCAG TACATCAAGT GTATCATATG CCAAGTACGC CCCCTATTGA 481 CGTCAATGAC GGTAAATGGC CCGCCTGGCA TTGTGCCCAG TACATGACCT TATGGGACTT 541 TCCTACTTGG CAGTACATCT ACGTATTAGT CATCGCTATT ACCATGGTCG AGGTGAGCCC 601 CACGTTCTGC TTCACTCTCC CCATCTCCCC CCCCTCCCCA CCCCCAATTT TGTATTTATT 661 TATTTTTTAA TTATTTTGTG CAGCGATGGG GGCGGGGGGG GGGGGGGNNN NNNNNNN // |
Primer: Cas9-C_F2 (Pr1051) Region: Cas9 3',nucleoplasmin NLS,bGHpA Sequence file: RDB19230_B1H2b.seq |
>19230_19230_B2DkF_2_Cas9-C_F2_H01_08_ABI08.ab1 1 NNNNNNNNNN NNNNNNGNNN CCNCCCTGNT CNNCCNGAGC ATCACCGGCC TGTACGAGAC 61 ACGGATCGAC CTGTCTCAGC TGGGAGGCGA CAAAAGGCCG GCGGCCACGA AAAAGGCCGG 121 CCAGGCAAAA AAGAAAAAGT AAGAATTCCT AGAGCTCGCT GATCAGCCTC GACTGTGCCT 181 TCTAGTTGCC AGCCATCTGT TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC CCTGGAAGGT 241 GCCACTCCCA CTGTCCTTTC CTAATAAAAT GAGGAAATTG CATCGCATTG TCTGAGTAGG 301 TGTCATTCTA TTCTGGGGGG TGGGGTGGGG CAGGACAGCA AGGGGGAGGA TTGGGAAGAG 361 AATAGCAGGC ATGCTGGGGA GCGGCCGCAG GAACCCCTAG TGATGGAGTT GGCCACTCCC 421 TCTCTGCGCG CTC // |
Primer: Cas9-N_R1 (Pr0650) Region: Cas9 5',SV40 NLS,3 x FLAGhybrid intron Sequence file: RDB19230_B1H2c.seq |
>D08293A5_B1H2_2_Cas9-N_R1_A01_01_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNGNAN TCGTCGGTGN NCACGGCCCN GCCCACAGAG 61 TTGGTGCCGA TGTCCAGGCC GATGCTGTAC TTCTTGTCGG CTGCTGGGAC TCCGTGGATA 121 CCGACCTTCC GCTTCTTCTT TGGGGCCATC TTATCGTCAT CGTCTTTGTA ATCAATATCA 181 TGATCCTTGT AGTCTCCGTC GTGGTCCTTA TAGTCCATGG TGGCACCGGT CCAACCTGAA 241 AAAAAGTGAT TTCAGGCAGG TGCTCCAGGT AATTAAACAT TAATACCCCA CCAACCAACC 301 ATCCCTTAAA CCCTTACCTC TTGCTCAGCT AATTACAGCC CGGAGGAGAA GGGCCGTCCC 361 GCCCGCTCAC CTGTGGGAGT AACGCGGTCA GTCAGAGCCG GGGCGGGCGG CGCGAGGCGG // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Nishimura, K., A super-sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair. Nucleic Acids Res. 48 (18): e108 (2020). PMID 32941625. |
---|
2023.05.01
GNP_filter3_RDBDEP_html_230501.pl