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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pAID1.2-CMV-linearizing in pX330

Expression vector of Cas9 protein and sgRNA to linearize pAID-CMV plasmids in vivo.

Catalog number RDB19230
Resource name pAID1.2-CMV-linearizing in pX330
Clone info. Expression vector of Cas9 protein and sgRNA to linearize pAID-CMV plasmids in vivo.
Vector backbone pX330-U6-Chimeric_BB-CBh-hSpCas9 (plasmid)
Size of vector backbone 4.3 kb
Selectable markers Ampicillin
Depositor|Developer Fukagawa, Tatsuo |
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External Database

            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Nishimura, K. et al. Nucleic Acids Res. 48 (18): e108, 2020).
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            Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
            Remarks
            The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Nishimura, K. et al. Nucleic Acids Res. 48 (18): e108, 2020)。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            専用MTA(CRISPR/Cas9内包遺伝子材料専用 非営利学術目的)をお使いください [Word]
            備考
            本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB19230 pAID1.2-CMV-linearizing in pX330 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pAID1.2-CMV-linearizing in pX330 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19230).

            Reference section:

            Nishimura, K., Yamada, R., Hagihara, S., Iwasaki, R., Uchida, N., Kamura, T., Takahashi, K., Torii, K.U., Fukagawa, T., A super-sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair. Nucleic Acids Res. 48 (18): e108 (2020). PMID 32941625.

            Further references such as user reports and related articles (go to bottom)


            References and tips

            Featured content

            Featured content Auxin Inducible Degron (AID) System (English text)
            Featured content Auxin Inducible Degron (AID) System (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB19230_B1H2p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: U6pro_F3 (Pr0648)
            Region: U6 pro,sgRNA for pAID-CMV,CMV en,chicken β-actin pro
            Sequence file: RDB19230_B1H2a.seq check
            >D08293A5_B1H2_2_U6pro_F3_E01_13_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNGG NNTATNNTAT GCTTACCGTA ACTTGNAAGT ATTTCGATTT
               61 CTTGGCTTTA TATATCTTGT GGAAAGGACG AAACACCGCC AAGTTCGGGT GAAGGCCCGT
              121 TTAAGAGCTA TGCTGGAAAC AGCATAGCAA GTTTAAATAA GGCTAGTCCG TTATCAACTT
              181 GAAAAAGTGG CACCGAGTCG GTGCTTTTTT GTTTTAGAGC TAGAAATAGC AAGTTAAAAT
              241 AAGGCTAGTC CGTTTTTAGC GCGTGCGCCA ATTCTGCAGA CAAATGGCTC TAGAGGTACC
              301 CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT
              361 GACGTCAATA GTAACGCCAA TAGGGACTTT CCATTGACGT CAATGGGTGG AGTATTTACG
              421 GTAAACTGCC CACTTGGCAG TACATCAAGT GTATCATATG CCAAGTACGC CCCCTATTGA
              481 CGTCAATGAC GGTAAATGGC CCGCCTGGCA TTGTGCCCAG TACATGACCT TATGGGACTT
              541 TCCTACTTGG CAGTACATCT ACGTATTAGT CATCGCTATT ACCATGGTCG AGGTGAGCCC
              601 CACGTTCTGC TTCACTCTCC CCATCTCCCC CCCCTCCCCA CCCCCAATTT TGTATTTATT
            661 TATTTTTTAA TTATTTTGTG CAGCGATGGG GGCGGGGGGG GGGGGGGNNN NNNNNNN
            //
            Primer: Cas9-C_F2 (Pr1051)
            Region: Cas9 3',nucleoplasmin NLS,bGHpA
            Sequence file: RDB19230_B1H2b.seq check
            >19230_19230_B2DkF_2_Cas9-C_F2_H01_08_ABI08.ab1
                1 NNNNNNNNNN NNNNNNGNNN CCNCCCTGNT CNNCCNGAGC ATCACCGGCC TGTACGAGAC
               61 ACGGATCGAC CTGTCTCAGC TGGGAGGCGA CAAAAGGCCG GCGGCCACGA AAAAGGCCGG
              121 CCAGGCAAAA AAGAAAAAGT AAGAATTCCT AGAGCTCGCT GATCAGCCTC GACTGTGCCT
              181 TCTAGTTGCC AGCCATCTGT TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC CCTGGAAGGT
              241 GCCACTCCCA CTGTCCTTTC CTAATAAAAT GAGGAAATTG CATCGCATTG TCTGAGTAGG
              301 TGTCATTCTA TTCTGGGGGG TGGGGTGGGG CAGGACAGCA AGGGGGAGGA TTGGGAAGAG
              361 AATAGCAGGC ATGCTGGGGA GCGGCCGCAG GAACCCCTAG TGATGGAGTT GGCCACTCCC
            421 TCTCTGCGCG CTC
            //
            Primer: Cas9-N_R1 (Pr0650)
            Region: Cas9 5',SV40 NLS,3 x FLAGhybrid intron
            Sequence file: RDB19230_B1H2c.seq check
            >D08293A5_B1H2_2_Cas9-N_R1_A01_01_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNGNAN TCGTCGGTGN NCACGGCCCN GCCCACAGAG
               61 TTGGTGCCGA TGTCCAGGCC GATGCTGTAC TTCTTGTCGG CTGCTGGGAC TCCGTGGATA
              121 CCGACCTTCC GCTTCTTCTT TGGGGCCATC TTATCGTCAT CGTCTTTGTA ATCAATATCA
              181 TGATCCTTGT AGTCTCCGTC GTGGTCCTTA TAGTCCATGG TGGCACCGGT CCAACCTGAA
              241 AAAAAGTGAT TTCAGGCAGG TGCTCCAGGT AATTAAACAT TAATACCCCA CCAACCAACC
              301 ATCCCTTAAA CCCTTACCTC TTGCTCAGCT AATTACAGCC CGGAGGAGAA GGGCCGTCCC
              361 GCCCGCTCAC CTGTGGGAGT AACGCGGTCA GTCAGAGCCG GGGCGGGCGG CGCGAGGCGG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Nishimura, K., A super-sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair. Nucleic Acids Res. 48 (18): e108 (2020). PMID 32941625.

            2023.05.01

            GNP_filter3_RDBDEP_html_230501.pl