RDB19231 - pAID-EF1a- linearizing in pX330

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pAID-EF1a- linearizing in pX330

Expression vector of Cas9 protein and sgRNA to linearize to pAID-EF1 alpha plasmids in vivo.

Catalog number	RDB19231
Resource name	pAID-EF1a- linearizing in pX330
Clone info.	Expression vector of Cas9 protein and sgRNA to linearize to pAID-EF1 alpha plasmids in vivo.
Vector backbone	pX330-U6-Chimeric_BB-CBh-hSpCas9 (plasmid)
Size of vector backbone	4.3 kb
Selectable markers	Ampicillin
Depositor\|Developer	Fukagawa, Tatsuo \|

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Reference sequence

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Nishimura, K. et al. Nucleic Acids Res. 48 (18): e108, 2020).

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Remarks
The BIOLOGICAL RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. Please refer the Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose).

提供案内 (日本国内) [open/close]

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Nishimura, K. et al. Nucleic Acids Res. 48 (18): e108, 2020).
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
Remarks	The BIOLOGICAL RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. Please refer the Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose).

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Nishimura, K. et al. Nucleic Acids Res. 48 (18): e108, 2020)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 専用MTA（CRISPR/Cas9内包遺伝子材料専用非営利学術目的）をお使いください [Word]
備考	本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。専用MTA（CRISPR/Cas9内包遺伝子材料専用非営利学術目的）をご参照ください。

Catalog #	Resource name	Availability	Shipping form	Fee (non-profit org.)
RDB19231	pAID-EF1a- linearizing in pX330	Under QC test. Please contact us.	DNA solution

Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References and tips

Electronic file

Original reference

original	Nishimura, K., A super-sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair. Nucleic Acids Res. 48 (18): e108 (2020). PMID 32941625.

Further references such as user reports and related articles (go to bottom)

Featured content	Auxin Inducible Degron (AID) System (English text)
Featured content	Auxin Inducible Degron (AID) System (Japanese text)

Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original	Nishimura, K., A super-sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair. Nucleic Acids Res. 48 (18): e108 (2020). PMID 32941625.

2022.03.21

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