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EGFP3 (#RDB19233)

Expression vector of tandemly oligomerized EGFP for diffusion analysis of proteins, multiple oligomeric EGFPn (n=3) with molecular weights of 90 kD.

Clone info. Expression vector of tandemly oligomerized EGFP for diffusion analysis of proteins, multiple oligomeric EGFPn (n=3) with molecular weights of 90 kD, which can be used as molecular rulers for quantification of protein mobility in the nucleus. See Pack, C.G. et al., Biophys. J., 91 (10): 3921-3936, 2006.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pEGFP-C1 (plasmid)
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cell)
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Kinjo, Masataka | Kitamura, Akira |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Pack, C.G. et al., Biophys. J., 91 (10): 3921-3936, 2006).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Pack, C.G. et al., Biophys. J., 91 (10): 3921-3936, 2006)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB19233 EGFP3 Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The EGFP3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19233).

Reference section:

Pack, C., Saito, K., Tamura, M., Kinjo, M., Microenvironment and effect of energy depletion in the nucleus analyzed by mobility of multiple oligomeric EGFPs. Biophys. J. 91 (10): 3921-3936 (2006). PMID 16950841. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Pack, C., Microenvironment and effect of energy depletion in the nucleus analyzed by mobility of multiple oligomeric EGFPs. Biophys. J. 91 (10): 3921-3936 (2006). PMID 16950841. [link to RRC of NBRP]