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px330-mC/Gm1140-Gm14692_Mid (#RDB19257)

Expression vector of sgRNA targeting interspace between Gm1140 and Gm14692 and hSpCas9-mouse Cdt1.

Clone info. Expression vector of sgRNA targeting interspace between Gm1140 and Gm14692 and hSpCas9-mouse Cdt1 fusion protein, cloned into the px330-mC (RDB14406).
Comment Activity was confirmed by the depositor. In vitro CRISPR-cutting pCAG-EGxxFP cloned sgRNA target sequence and checking EGFP signal.
Vector backbone px330-mC (RDB14406) (plasmid)
Size of vector backbone 8.8 kb
Selectable markers Ampicillin
Growth conditions LB+Ampicillin, 37oC
Gene/insert name mouse Gm1140 cDNA mouse Gm14692 cDNA
Depositor|Developer Mizuno, Seiya |
 

Distribution information

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Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Morimoto, K. et al., Sci. Rep., 10 (1): 9060, 2020).
Remarks
The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をお使いください [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Morimoto, K. et al., Sci. Rep., 10 (1): 9060, 2020)。
備考
本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Availability Shipping form Fee
RDB19257 px330-mC/Gm1140-Gm14692_Mid Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The px330-mC/Gm1140-Gm14692_Mid was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19257).

Reference section:

Morimoto, K., Numata, K., Daitoku, Y., Hamada, Y., Kobayashi, K., Kato, K., Suzuki, H., Ayabe, S., Yoshiki, A., Takahashi, S., Murata, K., Mizuno, S., Sugiyama, F., Reverse genetics reveals single gene of every candidate on Hybrid sterility, X Chromosome QTL 2 (Hstx2) are dispensable for spermatogenesis. Sci. Rep. 10 (1): 9060 (2020). PMID 32493902. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Morimoto, K., Reverse genetics reveals single gene of every candidate on Hybrid sterility, X Chromosome QTL 2 (Hstx2) are dispensable for spermatogenesis. Sci. Rep. 10 (1): 9060 (2020). PMID 32493902. [PubMed] [Article] [RRC of NBRP]