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pX459 gEEF1A1

Cas9 and guideRNA expression vector for cleavage of EEF1A1 gene.

Catalog number RDB19570
Resource name pX459 gEEF1A1
Clone info. Cas9 and guideRNA expression vector for cleavage of EEF1A1 gene. Used for knocking in to the end of EEF1A1 ORF.
Vector backbone PX459 (plasmid)
Gene/insert name synthetic DNA targeting human EEF1A1 3'UTR.
Depositor|Developer DNA Bank, | Nakade, Koji |
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External Database
human 20bp from EEF1A1 3'UTR
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.　(Nakade, K. et al., Cell Rep. Methods 3 (12): 100662 (2023))
          Additional terms and conditions:
          The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
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          Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
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          Remarks
          The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nakade, K. et al., Cell Rep. Methods 3 (12): 100662 (2023))。
          付加的提供条件：
          本件リソースの使用は学術機関での学術研究に限る。
          提供依頼
          提供依頼書 [Word]
          専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をお使いください [Word]
          手続きの概要は、「提供申込みについて[link]」をご覧ください。
          備考
          本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB19570 pX459 gEEF1A1 Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          How to cite this biological resource

          Materials & Methods section:

          The pX459 gEEF1A1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19570).

          Reference section:

          Nakade, K., Tsukamoto, S., Nakashima, K., An, Y., Sato, I., Li, J., Shimoda, Y., Hemmi, Y., Miwa, Y., Hayashi, Y., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. [link to RRC of NBRP]

          2024.04.04

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