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pUCtk2x2-OCT3/4-TEZ

Donor vector for the tdTomato reporter knock-in at the ORF end of OCT3/4. Selection of knocked-in clone using the double-tk system is available.

Catalog number RDB19572
Resource name pUCtk2x2-OCT3/4-TEZ
Alternative name pUCtk2x2-OCT3/4-TEBl
Clone info. Donor vector for the tdTomato reporter knock-in at the ORF end of OCT3/4. Selection of knocked-in clone using the double-tk system is available.
Vector backbone pUCtk2x2 (plasmid)
Gene/insert name Herpes simplex virus (HSV) thymidin kinase (TK) gene; human OCT3/4 genomic DNA; Dscosoma sp. red fluorescent protein (tdTomato); human EEF1A1 promoter; Streptoalloteichus hindustanus bleomycin resistant gene.
Depositor|Developer DNA Bank, | Nakade, Koji |
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External Database
human OCT3/4
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.　(Nakade, K. et al., Cell Rep. Methods 3 (12): 100662 (2023))
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nakade, K. et al., Cell Rep. Methods 3 (12): 100662 (2023))。
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          RDB19572 pUCtk2x2-OCT3/4-TEZ Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          How to cite this biological resource

          Materials & Methods section:

          The pUCtk2x2-OCT3/4-TEZ was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19572).

          Reference section:

          Nakade, K., Tsukamoto, S., Nakashima, K., An, Y., Sato, I., Li, J., Shimoda, Y., Hemmi, Y., Miwa, Y., Hayashi, Y., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. [link to RRC of NBRP]

          2024.04.04

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