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pX330_CDM45-1

Genome editing tool. sgRNA and SpCas9 expression in one vector. Subcloned an oligo nucleotide to target human H2BC21 gene stop codon nearby.

Catalog number RDB19579
Resource name pX330_CDM45-1
Alternative name pX330-gH2B
Clone info. Genome editing tool. sgRNA and SpCas9 expression in one vector. Subcloned 5'-gactcgagcgagcgagcgccsg-3' in between BbsI sites to target human H2BC21 gene stop codon nearby.
Vector backbone pX330 (plasmid)
Size of vector backbone 8.5 kb
Selectable markers Ampicillin
Gene/insert name synthetic human H2BC21 gene targeting gRNA.
Depositor|Developer DNA Bank, | Nakashima, Kenichi |
 
Sequence (full) RDB19579hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB19579_B2Ddp1-1.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.　(Nakade, K. et al., Cell Rep. Methods 3 (12): 100662 (2023))
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Remarks
The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nakade, K. et al., Cell Rep. Methods 3 (12): 100662 (2023))。
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提供依頼書 [Word]
専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をお使いください [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考
本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19579 pX330_CDM45-1 DNA solution

How to cite this biological resource

Materials & Methods section:

The pX330_CDM45-1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19579).

Reference section:

Nakade, K., Tsukamoto, S., Nakashima, K., An, Y., Sato, I., Li, J., Shimoda, Y., Hemmi, Y., Miwa, Y., Hayashi, Y., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. [link to RRC of NBRP]