Resource data sheet
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pCAG-mCherry

Expression vector of red fluorescent protein, mCherry.

Catalog number RDB19604
Resource name pCAG-mCherry
Clone info. Expression vector of red fluorescent protein, mCherry, SV40-polyA signal, CAG promoter.
Vector backbone pCAGpro-C1 (plasmid)
Selectable markers Kanamycin (E. coli), Neomycin (mammaliancell)
Gene/insert name Discosoma sp. RFP (mCherry)
Depositor|Developer Bito, Haruhiko | Sakamoto, Masayuki |
 
Sequence (full) RDB19604hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB19604_B2Fmp1-1.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Sakamoto, M., STAR Protoc. 3 (2): 101421 (2022)) designated by the DEPOSITOR is requested.
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MTA, for use for not-for-profit academic purpose [Word]
Please visit Information of Request for Distribution.[link] 
Remarks
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Sakamoto, M., STAR Protoc. 3 (2): 101421 (2022)) を引用する。
提供依頼
提供依頼書 [Word]
提供同意書 (MTA, 非営利学術目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考
Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19604 pCAG-mCherry DNA solution

How to cite this biological resource

Materials & Methods section:

The pCAG-mCherry was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19604).

Reference section:

Sakamoto, M., Ota, K., Kondo, Y., Okamura, M., Fujii, H., Bito, H., In utero electroporation and cranial window implantation for in vivo wide-field two-photon calcium imaging using G-CaMP9a transgenic mice. STAR Protoc. 3 (2): 101421 (2022). PMID 35693213. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Sakamoto, M., In utero electroporation and cranial window implantation for in vivo wide-field two-photon calcium imaging using G-CaMP9a transgenic mice. STAR Protoc. 3 (2): 101421 (2022). PMID 35693213. [link to RRC of NBRP]