Please review the QC test results indicated by icon below as well as clone information before placing your order.

er-(n2)oxStayGold(c4)

Vector for ER labeling with fluorescent protein oxStayGold

Sequence (full)	RDB19613zzk02.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Hirano, M. et al., Nat. Biotechnol. doi: 10.1038/s41587-022-01278-2, 2022. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] Approval form [Word] by Dr. Atsushi Miyawaki Please visit Information of Request for Distribution.[link]
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

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提供条件	1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Hirano, M. et al., Nat. Biotechnol. doi: 10.1038/s41587-022-01278-2, 2022. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 宮脇敦史博士による提供承諾書 [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考	提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19613_B2Eap1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro,insert 5' (signal seq,(n2)oxStayGold(c4) 5') Sequence file: RDB19613_B2Eaa.seq
>D08836A9_B2Ea_1_CMV_Forward_F06_18_ABI24.ab1     1 NNNNNNNNNN NNNCNTTGNN NNNNNNGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA    61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG   121 ACTCACTATA GGGAGACCCA AGCTTGCGGC CGCCACCATG CTGCTGCCCG TCCCCCTGCT   181 GCTGGGCCTG CTGGGCGCCG CCGCGGATCC GATGGCTAGT AAGGGCGAGG AGCTGTTCAC   241 CGGGGTGACA CCATTTAAAT TTCAACTTAA AGGAACCATC AATGGCAAAT CGTTTACCGT   301 TGAAGGCGAA GGTGAAGGAA ATTCACATGA AGGTTCTCAT AAAGGAAAAT ATGTTTGTAC   361 AAGTGGAAAA CTACCGATGT CATGGGCAGC ACTTGGAACA TCCTTTGGTT ATGGAATGAA   421 ATATTATACC AAATATCCTA GTGGACTGAA GAACTGGTTT CATGAAGTAA TGCCTGAAGG   481 CTTTACCTAC GATCGTCATA TTCAATATAA AGGCGATGGG AGTATCCATG CAAAACACCA   541 ACACTTTATG AAAAATGGGA CTTATCACAA CATTGTTGAA TTTACTGGTC AGGATTTTAA   601 AGAAAATAGT CCAGTCTTAA CTGGAGATAT GAATGTCTCA TTACCGAATG AAGTTCAACA   661 TATACCCAGA GATGATGGAG TAGAATGCCC AGTGACCTTG CTTTATCCTT TATTATCGGA   721 TAAATCAAAA TGCGTTGAGG CTTACCAAAA TACAATCATC AAGCCTCTTC ATAATCAACC   781 AGCACCTGAT GTCCCATATC ACTGGATTCG TNNACAATAC ACACAAAGCA AAGANGANAC   841 CGANGAACGT GATCATATTA TTCNATCNNA NNNTCTCGAA NCACACTTAC NNTGNCATGA   901 NNCTTCAGCA TCGGCAGTAA AGGANGAACT GNAAGAANTC TGCANANATC NNTCACACTG   961 GCGGNNGCTC NNNCATGNNT CNAGANGNNC NNATTCTANN NNGNNNNCNN ANNGNTANNN 1021 NNNGNNNANN ANNNNNNNNN N //
Primer: bGH_rev3 (Pr0885) Region: insert 3' ((n2)oxStayGold(c4) 3') Sequence file: RDB19613_B2Eab.seq
>19613_19613_B2Ea_1_bGH_rev3_D01_04_ABI08.ab1     1 NNNNNNNNNN NNGNNNNGGG NNGGGNANNN ACNGANGGCT GGCAACTAGA AGGCACAGTC    61 GAGGCTGATC NNCGAGCTCT AGCATTTAGG TGACACTATA GAATAGGGCC CTCTAGATGC   121 ATGCTCGAGC GGCCGCCAGT GTGATGGATA TCTGCAGAAT TCTTACAGTT CATCCTTTAC   181 TGCCGATGCT GAAGGCTCAT GCCAAGGTAA GTGTGCTTCG AGAGTCTCTG ATTGAATAAT   241 ATGATCACGT TCCTCGGTAT CATCTTTGCT TTGTGTGTAT TGTTTACGAA TCCAGTGATA   301 TGGGACATCA GGTGCTGGTT GATTATGAAG AGGCTTGATG ATTGTATTTT GGTAAGCCTC   361 AACGCATTTT GATTTATCNN NNNATAAAGG ANAAAGCNAG GTCNCTGGGN NTTCNACTNC 421 NTCNTCNCTG GGTANATGTT GAANNTNNNT TNNGNANNGN NNNNNNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,NeoR Sequence file: RDB19613_B2Eac.seq
>D08836A9_B2Ea_1_SV40pro_ori_F_H06_24_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNCG CCTCTGCCTC TGANCTATTC NNGAAGTAGT GAGGAGGCTT    61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC   121 TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG   181 TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG   241 CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA   301 GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT   361 GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA   421 CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC   481 CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC   541 CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC   601 CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT   661 GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA   721 TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG   781 CCGGCTGGGT GTGGCGGANC GCTATCAGGA CATAGCGTTG GCTACNCGTG ATATTGCTGA   841 AGANCTTGGC GGCGAATGNG CTGANCGCTT CCTCGNGCTT TACGGNATCG CCGCTCCCGA   901 NTCNCANCGC ATCGCCTTCT ATCGCCTTCT NGANNANTTC TTCNGANCGG GNNTCTGGGG   961 TTCNAAATGA CCGANCNAGC GANNCCNNAN CNNNNNNNCN CNANNTTTCN ANTCCNCCNN 1021 CNNNNNNNNN N //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.01.29

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