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pCAG-Dcst1-3xFLAG

Expression vector of mouse Dcst1-3xFLAG under the CAG promoter.

Catalog number RDB19670
Resource name pCAG-Dcst1-3xFLAG
Clone info. Expression vector of mouse DC-STAMP domain containing 1 (Dcst1) tagged with 3xFLAG at C-terminus.
Comment Expression was confirmed by the depositor with Western blotting.
Vector backbone pCAG1.1 (plasmid)
Selectable markers Ampicillin
Gene/insert name mouse Dcst1 cDNA
Depositor|Developer Ikawa, Masahito | Noda, Taichi |
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External Database
mouse Dcst1

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Noda, T. et al. Commun. Biol. 7;5(1): 332, 2022) The RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
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          MTA, for use for not-for-profit academic purpose [Word]
          MTA, for use for for-profit purpose [Word]
          Please visit Information of Request for Distribution.[link] 
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Noda, T. et al. Commun. Biol. 7;5(1): 332, 2022)。利用者が非営利目的の教育・研究以外の目的に用いる場合は、大阪大学と別途MTAを
          締結すること。
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          提供依頼書 [Word]
          提供同意書 (MTA, 非営利学術目的用)[Word]
          提供同意書 (MTA, 営利目的用)[Word]
          手続きの概要は、「提供申込みについて[link]」をご覧ください。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB19670 pCAG-Dcst1-3xFLAG Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          How to cite this biological resource

          Materials & Methods section:

          The pCAG-Dcst1-3xFLAG was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19670).

          Reference section:

          Noda, T., Blaha, A., Fujihara, Y., Gert, K.R., Emori, C., Deneke, V.E., Oura, S., Panser, K., Lu, Y., Berent, S., Kodani, M., Cabrera-Quio, L.E., Pauli, A., Ikawa, M., Sperm membrane proteins DCST1 and DCST2 are required for sperm-egg interaction in mice and fish. Commun. Biol. 5 (1): 332 (2022). PMID 35393517. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


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          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Noda, T., Sperm membrane proteins DCST1 and DCST2 are required for sperm-egg interaction in mice and fish. Commun. Biol. 5 (1): 332 (2022). PMID 35393517. [link to RRC of NBRP]

          2023.10.05

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