RDB19704 - pEGFP-C1-hRUTBC3-R165A

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pEGFP-C1-hRUTBC3-R165A

Retroviral expression vector of human RUTBC3 GAP activity-deficient mutant tagged with EGFP at N-terminus.

Catalog number	RDB19704
Resource name	pEGFP-C1-hRUTBC3-R165A
Clone info.	Retroviral expression vector of human RUTBC3 (RUN and TBC1 domain-containing protein 3) GAP activity-deficient R165A mutant tagged with EGFP at N-terminus. See Fig.3A of Hiragi, S. et al. (2022) J. Cell Biol. 221, e202201114
Vector backbone	pEGFP-C1 (plasmid)
Size of vector backbone	4.7 kb
Selectable markers	Kanamycin (E. coli), Neomycin (mammalian cell)
Gene/insert name	human SGSM3 cDNA
Depositor\|Developer	Fukuda, Mitsunori \|

Other clones in our bank

human SGSM3

External Database
human SGSM3

Reference sequence

Sequence (full)	RDB19704zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Itoh, T. et al. Genes Cells. 11, 1023-1037, 2006)

提供案内 (日本国内) [open/close]

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Itoh, T. et al. Genes Cells. 11, 1023-1037, 2006)

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Itoh, T. et al. Genes Cells. 11, 1023-1037, 2006)。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB19704	pEGFP-C1-hRUTBC3-R165A	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-hRUTBC3-R165A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19704).

Reference section:

Hiragi, S., Matsui, T., Sakamaki, Y., Fukuda, M., TBC1D18 is a Rab5-GAP that coordinates endosome maturation together with Mon1. J. Cell Biol. 21 (12): e202201114 (2022). PMID 36197338.

Further references such as user reports and related articles (go to bottom)

References and tips

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB19704_B2Jjp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402) Region: EGFP 3',insert 5' Sequence file: RDB19704_B2Jja.seq
>D09032A1_B2Jj_1_EGFP-C2_A01_01_ABI24.ab1 1 NNNNNNNNNN NNNNNCGNNC NCNTGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC 61 TCTCGGCATG GNNNAGCTGT ACAAGTCCGG ACTCAGATCC ATGTCAGGAA GCCATACACC 121 TGCCTGTGGC CCTTTCTCAG CCCTGACTCC GAGCATATGG CCCCAGGAGA TCTTGGCCAA 181 GTACACGCAG AAGGAAGAGT CAGCAGAGCA ACCAGAGTTC TACTACGATG AGTTTGGTTT 241 CCGTGTGTAC AAGGAAGAAG GTGATGAGCC TGGCTCCAGT CTGCTGGCGA ACTCCCCTCT 301 GATGGAGGAT GCTCCACAGA GGCTGCGGTG GCAGGCCCAC CTGGAGTTCA CCCATAACCA 361 CGATGTGGGG GATCTCACCT GGGACAAGAT TGCCGTCTCC CTACCCCGCT CTGAGAAGCT 421 CCGCTCCCTG GTGCTGGCCG GCATCCCACA TGGCATGAGG CCACAGCTGT GGATGCGGCT 481 CTCTGGGGCC CTGCAGAAGA AGAGGAACTC TGAGCTGTCC TACCGCGAGA TTGTGAAGAA 541 CAGCTCCAAC GATGAGACCA TCGCTGCCAA GCAGATCGAG AAGGACCTGC TCGCCACCAT 601 GCCCAGCAAC GCCTGCTTCG CCAGCATGGG TAGCATCGGG GTACCCCGCC TGCGCAGGGT 661 GCTCCGGGCC CTGGCCTGGC TCTACCCAGA GATCGGCTAC TGCCAGGGCA CCGGCATGGT 721 GGCCGCCTGC CTCCTGCTGT TCCTGGAGGA NGANGANGCC TTCTGGATGA NGTCTGCCAT 781 CATCGAGGAC CTGCTCCCCG CCTCCTACTT CAGCACCACC CTGCTGGGTG TCCAGACTGA 841 NCAGCGGGTC CTGCGCCACC TCATTGTCCA GTATCTGCCT CGCCNGGACA AGCTGCTCCA 901 GGANCATGAC ATTNAGCTGT CCCNGATCAC ACTGCACTGG TTNCTCACNG CCTTCNCNNG 961 CGNGGNNGAN NTCAAGCTGC TNCNGCNCNT NNGGNNNCNG TTTTTCNNCN ANGGNTNCNN 1021 GGNNCTNNNN NNNCNNANNN NNGNNNNNNN NNN //
Primer: SV40polyA_R2 (Pr0623) Region: insert 3' Sequence file: RDB19704_B2Jjb.seq
>D09032A1_B2Jj_1_SV40polyA_R2_B01_04_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNGTTAA CAACNACAAT TGCATTCATT TTATGTTTCA 61 GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT 121 GGCTGATTAT GATCAGTTAT CTAGATCCGG TGGATCCCGG GCCCGCGGTA CCGTCGACCT 181 GCAGGCGGCC GCTATGGTCG ACTGCAGAAT TCGATTTCAC CCGTCCACAT CCCAGCTGAA 241 GAGGTGGTGC TTCACCAGCA TGTCCCGGAC GCCCTCCTTC AGGGGCTGCT GCGCCTCTCT 301 CTTCGCAGGG AGCTCCCAGT CCTGGGAGAG GCTGAAGGCA AAGCAGCAGA GGACTCGGAG 361 CTCACACTTG ATCTGGACCC AGCCCGGGCT GCGCAGGAAG GACCAGGGCT GGTACCACTT 421 CTCCACGGTG GGCAGGCTGG AGCAGAGCAC CTCCAGCCAC AGGTGCAGCA CCTGCTCATT 481 GAGCCCCACG CAGATCAGTG AGCGGAGCTT CACATCCATT TGTGCATGCA CTGCATCGTG 541 GGTCACGTTC ACAGACTGCA CAGCCCGGTA GAGCAGCTCC TCCGGGGTCA GGACTTTGCC 601 ATCTTCATCC AACCTGAAGG TCTTACAGAG CACCAGACGG GAATACACGG AGGCAAAGTC 661 TCTCTCGACC TCCCGGCCTG CAGCCTCCTC GATAAACAGC CAGGGGTGGC AGGCGCCCCC 721 AAGCAGGGAT GGCTTCTTCA GTCCATGTTC GAACAGGGCC TTAANGGCCG GGCAGAGGGT 781 CCCTCGCACG ANGTCTGTGA CCCCCTCCGT CACCGAGTCA TCCCCCGCGA TGGAGTACTC 841 TTTGCTGCGC TCATCCAGGA NTTCCACGAA CTTGGCTGGA AANCAGCCTC GCNNNCGNTG 901 ANCTCCCCCA CCCANCAGTG CTCGTNCTTC TGANACACGA TTGTGATGAT GTCNTTCTTG 961 CNGANNCCCA GCTCGTCGTC GTCNNGNCNC NCAAAGTCCA GCNNNNNNNN NN //
Primer: CMV_Forward (Pr0016) Region: CMV pro,EGFP 5' Sequence file: RDB19704_B2Jjc.seq
>D09032A1_B2Jj_1_CMV_Forward_B02_02_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NGNNNNNAAT GGGCGGTAGG CGTGTACGGT GGGNNGTCTA 61 TATAAGCAGA GCTGGTTTAG TGAACCGTCA GATCCGCTAG CGCTACCGGT CGCCACCATG 121 GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC 181 GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC 241 AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC 301 GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG 361 CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC 421 AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG 481 AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG 541 CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC 601 ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC 661 CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC 721 CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG 781 CTGGAGTTCG TGACCGCCGC CNGGATCACT CTCGGCATGG ACGANCTGTA CAAGTCCGGA 841 CTCAGATCCA TGTCANGAAG CCATACACCT GCCTGTGGCC CTTTCTCAGC CCTGACTCCG 901 ANCATATGGN CCCAGGANAT CTTGNCCAAG TACACGCAGA ANGAANANTC ANCNNANCAN 961 CNANNNTTCT ANTACNATNA NTTTGNTTTC CGNGNGNNNN NNNAANNANN NNGNNNANCN 1021 NNNNNNCNNN NNNNNNN //
Primer: VN_F1 (Pr0992) Region: EGFP 3',insert 5' Sequence file: RDB19704_B2Jjd.seq
>D09032A1_B2Jj_1_VN_F1_C02_03_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNTC TATATCATGG CCGANAAGCA GAAGAACGGC 61 ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC 121 CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC 181 CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG 241 CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTCCGGA 301 CTCAGATCCA TGTCAGGAAG CCATACACCT GCCTGTGGCC CTTTCTCAGC CCTGACTCCG 361 AGCATATGGN CNNNNGAGAT CTTGGCCAAG TACACGCAGA AGGAAGAGTC AGCAGAGCAA 421 CCAGAGTTCT ACTACGATGA GTTTGGTTTC CGTGTGTACA AGGAAGAAGG TGATGAGCCT 481 GGCTCCAGTC TGCTGGCGAA CTCCCCTCTG ATGGAGGATG CTCCACAGAG GCTGCGGTGG 541 CAGGCCCACC TGGAGTTCAC CCATAACCAC GATGTGGGGG ATCTCACCTG GGACAAGATT 601 GCCGTCTCCC TACCCCGCTC TGAGAAGCTC CGCTCCCTGG TGCTGGCCGG CATCCCACAT 661 GGCATGAGGC CACAGCTGTG GATGCGGCTC TCTGGGGCCC TGCAGAAGAA GAGGAACTCT 721 GAGCTGTCCT ACCGCGAGAT TGTGAAGAAC AGCTCCAACG ATGAGACCAT CGCTGCCAAG 781 CAGATCGAGA ANGANCTGCT CGCCNCCATG NNCAGCAACG CCTGCTTCGC CAGCATGGGT 841 AGCATCGGGG TACCCCGCCT GCGCAGGGTG CTNCGGGNCC TGGCCTGNCT CTACCNAGAG 901 ATCGGCTACT GCCAGGGCAC CNGNATGGTG NNCGCCTGCC TCCNGCTGNN NNNNGNNGAN 961 GAGNNNNCCT NNNNGNNGAN GTCTNNCNTC NTNNAGGNNC NGCTCCNNNC CTNCNANTTN 1021 NNNANNNCCN NNNNNGNNNN NCAGANNNNN NNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Hiragi, S., TBC1D18 is a Rab5-GAP that coordinates endosome maturation together with Mon1. J. Cell Biol. 21 (12): e202201114 (2022). PMID 36197338.
reference	Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123.

2023.05.22

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