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pMRXIP-VPS16-GFP (#RDB19787)

Retroviral vector for stable expression of human VPS16 with C-terminal EGFP.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Jiang, P., Mol. Biol. Cell 25 (8): 1327-1337 (2014). PMID 24554770. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Retroviral vector for stable expression of human VPS16 with C-terminal EGFP.
Vector backbone pMRXIP GFP (plasmid)
Selectable markers Ampicillin (E. coli), Puromycin (mammalian cell)
Growth conditions LB+Amp, 37oC
Gene/insert name human VPS16 cDNA; Aequorea victoria GFP (EGFP).
Depositor|Developer Mizushima, Noboru |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Jiang, P. et al. Mol. Biol. Cell. 25(8): 1327-1337, 2014)
Additional terms and conditions:
Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Jiang, P. et al. Mol. Biol. Cell. 25(8): 1327-1337, 2014)。
付加的使用条件：
pMRXベクターについて：利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee
RDB19787 pMRXIP-VPS16-GFP DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pMRXIP-VPS16-GFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19787).

Reference section:

Jiang, P., Nishimura, T., Sakamaki, Y., Itakura, E., Hatta, T., Natsume, T., Mizushima, N., The HOPS complex mediates autophagosome-lysosome fusion through interaction with syntaxin 17. Mol. Biol. Cell 25 (8): 1327-1337 (2014). PMID 24554770. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Jiang, P., The HOPS complex mediates autophagosome-lysosome fusion through interaction with syntaxin 17. Mol. Biol. Cell 25 (8): 1327-1337 (2014). PMID 24554770. [PubMed] [Article] [RRC of NBRP]