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pMXs-puro-GFP-p62 K7A, D69A (#RDB19794)

Retroviral vector for stable expression of mouse p62 mutant (K7A/D69A) with N-terminal GFP.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Itakura, E., J. Cell Biol. 192 (1): 17-27 (2011). PMID 21220506. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Retroviral vector for stable expression of mouse p62 mutant (K7A/D69A) with N-terminal GFP.
Vector backbone pMXs-puro (plasmid)
Selectable markers Ampicillin (E. coli), Puromycin (mammalian cell)
Growth conditions LB+Amp, 37oC
Gene/insert name mouse Sqstm1 cDNA; Aequorea victoria GFP (EGFP).
Depositor|Developer Mizushima, Noboru |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Itakura, E. Mizushima, N. J. Cell Biol. 192(1): 17-27, 2011)
Additional terms and conditions:
Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Itakura, E. Mizushima, N. J. Cell Biol. 192(1): 17-27, 2011)。
付加的使用条件：
pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee
RDB19794 pMXs-puro-GFP-p62 K7A, D69A DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pMXs-puro-GFP-p62 K7A, D69A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19794).

Reference section:

Itakura, E., Mizushima, N., p62 Targeting to the autophagosome formation site requires self-oligomerization but not LC3 binding. J. Cell Biol. 192 (1): 17-27 (2011). PMID 21220506. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Itakura, E., p62 Targeting to the autophagosome formation site requires self-oligomerization but not LC3 binding. J. Cell Biol. 192 (1): 17-27 (2011). PMID 21220506. [PubMed] [Article] [RRC of NBRP]